[Dynamics of mental disorders after extracerebral implantation of embryonal nervous tissue in epilepsy]. / Dinamika psikhicheskikh narushenii posle ékstratserebral'noi implantatsii émbrional'noi nervnoi tkani pri épilepsii.

The paper presents the method elaborated by the authors for production, storage and implantation of embryonal nervous tissue (ENT). Extracerebral implantation was performed in 33 epileptic patients aged 2-58 years. Clinical state of the patients and EEG alterations were studied. Before treatment the patients' mental status was characterized by affective disorders, dysphoria, depression, exhaustion of mental processes, varying intellectual-mnestic decrease and behavioral disinhibition. Oligophrenia as of debility was observed in several cases. After implantation the pronounced positive changes in both paroxysmal states and overall mental status were observed in all the cases irrespective of their age of the patients. These clinical changes correlated with the dynamics of EEG changes (EEG changes were not observed in 2 individuals with positive clinical effects).

[Immunomorphological changes in the mouse brain after intracerebral administration of a neurotropic strain of influenza virus]. / Immunomorfologicheskie izmeneniia v golovnom mozge myshei pri intratserebral'nom vvedenii neirotropnogo shtamma virusa grippa.

The work dealt with intracerebral inoculation of 150 mice with neurotropic strain of influenza virus A/WSN/33. Virological and immunohistochemical study of virus localization in the mice brain was carried out. It was shown that virus reproduction in ependymal lining cells and plexus choroideus epithelium is followed by destruction of cells and uptake of disintegration products by macrophages. Since the influenza infection is followed by a large number of macrophages involved in gene expression of the main histocompatibility complex, the development of autoimmune reaction with the formation of autoantibodies to brain specific antigens can't be ruled out.

[Subcellular mechanisms of the action of weak extra-low frequency electromagnetic fields on the cerebral cortex]. / Subkletochnye mekhanizmy vozdeistviia slabykh sverkhnizkochastotnykh élektromagnitnykh polei na koru golovnogo mozga.

Electron microscopic examinations of the cerebral cortex in rabbits and rats after exposure to weak extra-low electromagnetic fields has detected ultrastructural changes whose degree in different nerve tissue elements was dependent on the duration of irradiation. Single exposure to weak extra-low electromagnetic field resulted only in the glial response of the neocortex, while multiple exposure resulted alternately in the response of glial cells or neuronal bodies. Three days after a single exposure and within 15 days after multiple exposure to electromagnetic field a complete repair of the nervous tissue was observed. No destructive or pathological alterations have been found which suggests the functional character of the reactions induced. Neuro-morphological alterations are of a similar character when induced by weak extra-low electromagnetic fields or other biophysical effects which points to common subcellular mechanisms of nonspecific adaptive reactions.

[Ultrastructural reorganization of components of the hemato- and cerebrospinal fluid-encephalic barriers following transcranial micropolarization]. / Ul'trastrukturnye perestroiki komponentov gemato- i likvoroéntsefalicheskogo bar'erov pri transkranial'noi mikropoliarizatsii.

It has been established that transcranial micropolarization causes ultrastructural changes in the intracerebral components of the blood- and CSF-brain barriers. The changes observed in the electron density of the limiting and basal membranes, edema of the processes, changes in the vascular endothelium and in the intercellular fissures of the brain are characterized by a reactive rather than a destructive character. An assumption is discussed that an increase in barrier permeability can be explained by the influence of micropolarization, which may in turn lead to altered properties in the cerebral internal medium itself. Transcranial microdepolarization is recommended for correcting the physiological processes in the brain and for altering the permeability of the blood- and CSF-brain barriers.

[Ultrastructural changes in the cerebral cortex following transcranial micropolarization]. / Ul'trastrukturnye izmeneniia kory golovnogo mozga posle transkranial'noi mikropoliarizatsii.

Electron microscopy of the cerebral cortex in cats and monkeys following transcranial micropolarization (TCMP) demonstrated ultrastructural changes whose degree was dependent on the electric current intensity and the stimulation period. In the focus of stimulation the current affected the brain tissue directly, different elements of the cerebral cortex showing unegual sensitivity to various TCMP regimens. The glia was the first to respond, then the neuronal bodies, and the last -- the synaptic structures. In the areas distant from the TCMP focus synaptic components altered first. The ultrastructural changes revealed were not of pathological character.

[Detection of alpha 1-antitrypsin in unfertilized ova of albino rats]. / Obnaruzhenie alpha 1-antitrypsin v neoplodotvorennykh iaitsakh belykh krys.

500--700 rat oocytes were solubilized in 0.3--0.6 ml of 1% sodium dodecyl sulphate (SDS) --0.06 tris-HCl, pH 6.7. The excess of SDS was removed in the form of unsoluble Ba2 (SDS) and the lysate was placed on the surface of 1% agarose microgel, performed in a glass capillar with the inner diameter 600 and the length of gel 7--8 nm. The microvolume (1.3 mcl) of monospecific antiserum against alpha1-human antitrypsin was placed on the opposite surface of the agarose gel. After 48 hours, the amount of microimmunoprecipitate was determined by staining the gel with Amido Black 10 B, followed by quantitative scanning using a double wave microspectrophotometer. The mean value of alpha1-antitrypsin was about 2.3+/-1.4 pcg per ovum. It is supposed that this antienzyme may terminate the "zone" and "cortical" reactions during the fertilization in mammals.

[A study of the protein composition of the zona pellucida of mammalian oocytes and zygotes by a method of microelectrophoresis in polyacrylamide gel]. / Izuchenie belkovogo sostava blestiashchei obolochki ootsitov i zigot mlekopitaiushchikh metodom mikroélektroforeza v poliakrilamidnom gele

Z. pellucide from oocytes and zygotes of CBA mice and albino rats were lysed in a microvolume of 1% buffered sodium dodecylsulphate (SDS). The microlysate (about 1,0--2,0-10(-7) g protein) was fractionated by means microdiscelectrophoretic technique, using 10%- and 15%-polyacrylamide gel containing 0.1% SDS. The new proteins with increased anodic mobility was found among zonal proteins after fertilization in CBA mice and rats. Homogenous fraction of glycoprotein (approximately molecular weight 44 000) is found in z. pellucida of rat oocytes. This glycoprotein fraction from rat zygote coats produces 3 components moving to anode under electrophoresis. The data obtained support the hypothesis that the mechanism of "zonal fraction" under fertilization in mammals can be due to partial lysis of coat proteins by proteinase of cortical granules.

[Molecular weight characteristics of the proteins of oocytes and of cleavage ova in CBA mice by means of capillary microdisc electrophoresis]. / Molekuliarno-vesovaia kharakteristika belkov ootsitov i drobiashchikhsia iaitsekletok myshei CBA metodom kapilliarnogo mikrodiskelektroforeza

5-20 oocytes or cleaving embryos of CBA mice were dissolves in 0.2-0.5 ml of 1% buffered sodium dodecylsulfate (SDS), and soluble proteins were separated using electrophoresis in capillaries filled with PAA-SDS-gel. The method enabled us to calculate the approximate molecular weight of proteins by relative mobilities and allowed to determine 1-5 mmcg protein in a single band. 7 groups of proteins were identified in mouse oocytes using 10% PAA-0.1% SDS-gel with the following molecular weights: 208 000, 206 000, 155 000, 112 000, 59 000, 40 000 and 30 000, resp. The same groups of protein were discovered in zygotes and cleaving mouse embryos. The molecular weight distribution of low-molecular weight basic proteins of CBA mice is species-specific and reveals a qualitative changes in the early embryogenesis.

[Determination of protein concentration in cleaving mammalian ova by the capillary spectrophotometry method]. / Opredelenie soderzhaniia belka v drobiashchikhsia iaitsekletkakh mlekopitaiushchikh metodom kapilliarnoi spektrofotometrii

A micromodification of the Lowry's method is described which allows to measure reliably the protein content in 5-20 embryos of white rats and CBA mice. Differences in the protein content in rat embryos at the stages of 2 blastomeres and blastocyst were shown to be statistically unreliable (20.2 +/- 1.4 and 18.9 +/- 1.3 ng, resp.). The protein content in the mouse embryos at the same two stages differs reliably (19.1 +/- 1.1 and 22.0 +/- +/- 1.7 ng, resp.). The protein content in zona pellucida does not differ reliably from those in rat embryos both at the stages of 2 blastomeres (4.5 ng) and blastocyst (2.3 ng). The protein content in embryos devoid of zona pellucida decreased after 3 hours incubation in the medium 199 at 37 degrees at the stages of morula and blastocyst by 17-20% in rats and by 50% in mice. Addition of 1% serum albumin to the incubation medium did not prevent the partial "loss" of protein by the embryos. The protein content in the rat and mouse embryos at the stage of 2 blastomeres suffered no changes under long-term incubation in the medium 199.

Detection of lactate dehydrogenase isoenzymes in single mammalian oocytes during cleavage by a micromodivication of disc electrophoresis.

A micromodification of the method of disc-electrophoresis in glass capillary tubes is described. It can be used to study the protein composition and activity of lactate dehydrogenase isoenzymes in single rat and mouse oocytes during clevage.

[Constitutive proteins of the oocytes and early embryos of rats]. / Konstitutivnye belki ootsitov i rannikh zarodyshei krys

5 protein fractions were identified and their relative mobility was determined in the rat oocytes and cleaving embryos by means of vertical capillary microdisc-electrophoresis in 7.5% polyacrilamide gel (PAA-gel). The same fractions were identified in the cleaving embryos devoid of zona pellucida. A conclusion was drawn that these proteins were present in the oocyte cytoplasm and kept in the cleaving embryos until the stage of implantation. 4 groups of proteins with different anodic mobility were identified in the isolated zona pellucida by means of microdisc-electrophoresis in 7.5% PAA-gel added with 1% sodium dodecylsulfate (SDS). The molecular weight of low molecular weight proteins of oocytes and preimplantation embryos was determined by means of disc-electrophoresis in 14% PAA-gel with 1% SDS. The zona pellucida of one embryo contained, according to the data of capillary spectrophotometry, 5 ng of protein.