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INTRODUCTION: The spread of coronavirus 2019 (COVID-19) is a serious problem all over the world. Several immunochromatography kits of the antibody for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) have been developed, but it is still unclear which kits have high diagnostic value. This study aims to evaluate the accuracy rate for antibody detection of each immunochromatography kit and reveal which kit has a high diagnostic value for antibody detection. METHODS: This study was carried out between 1 August 2020 and 14 October 2020 at the Association of EISEIKAI Medical and Healthcare Corporation Minamitama Hospital. Patients diagnosed with COVID-19 and approximately 30 days after symptom onset were included as the positive group. The serum SARS-CoV-2 antibodies were analysed using seven immunochromatography kits. RESULTS: Twenty samples (Positive group: 10 patients, Negative group: 10 healthy medical workers) were included in this study. The median age of the patients was 44 years, and the median duration from symptom onset was 30.5 days in the positive group. The accuracy rates for IgM/IgG detection were: 90.0%/100% in Kit A; 50.0%/95.0% in Kit B; 55.0%/65.0% in Kit C; 60.0%/55.0% in Kit D; 50.0%/80.0% in Kit E; 80.0%/90.0% in Kit F; and 90.0%/100% in Kit G. CONCLUSIONS: Our study showed that there is a variation of accuracy rates between immunochromatography kits for antibodies of SARS-CoV-2. COVID-19 IgG/IgM RAPID TEST CASSETTE (Hangzhou Biotest Biotech Co., Ltd., China) and Nadal COVID-19 IgG/IgM Rapid Test (BioServUK Ltd., UK: United Kingdom) have high accuracy rates for both IgM and IgG detection. Evidence from large population studies of immunochromatography kits is needed to clarify the details of diagnostic value for SARS-CoV-2.
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Fluorescence excitation spectra of dibenzofuran in a supersonic jet are observed and the vibronic structure is analyzed for the S(1) (1)A(1) (pipi) and S(0) states. An observation of the rotational envelopes reveals that the band is a B-type band. However, it is shown that most of the strong vibronic bands are A-type bands. The intensity arises from vibronic coupling with the S(2) (1)B(2) state. We find a broad emission in the dispersed fluorescence spectrum for the excitation of the high vibrational levels in the S(1) state. This indicates that intramolecular vibrational redistribution (IVR) occurs efficiently in the isolated dibenzofuran molecule.
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ATP, an important and ubiquitous extracellular signaling molecule, is often released by mechanical stimuli and plays an essential role in mechano-signaling. In lactating mammary glands, secretory epithelial (SE) cells form alveoli in which milk is held, and myoepithelial (ME) cells surrounding the alveoli contract in response to oxytocin to expel milk. Previously we found that the contraction of ME cells worked as a mechanical stress to SE cells and caused ATP-release in cultured mammary epithelial cells. The released ATP activated P2Y2 in surrounding SE cells and P2Y1 in ME cells. We already reported that ATP synergistically enhanced oxytocin response in ME cells. These findings mean that ME and SE cells interact mutually via released ATP to enhance the milk ejection. Recently, we found that cell-stretch also induced Ca(2+)-increases and ATP-release. The stretching of alveoli should occur by milk filling. So, only the milk-filled alveoli (but not empty alveoli) are surrounded by ATP. The ATP lowers the threshold of the oxytocin receptors and enables the milk-filled alveoli to contract in response to oxytocin at a concentration in the blood. Slight but apparent constitutive-ATP-release was observed in non-stimulated cells and the release was enhanced in Ca(2+)-free solution. The pathway of ATP-release is not yet clear, but pharmacologically, there seems to be two or more pathways.
