Recent advances in the design of implantable insulin secreting heterocellular islet organoids.

Islet transplantation has proved one of the most remarkable transmissions from an experimental curiosity into a routine clinical application for the treatment of type I diabetes (T1D). Current efforts for taking this technology one-step further are now focusing on overcoming islet donor shortage, engraftment, prolonged islet availability, post-transplant vascularization, and coming up with new strategies to eliminate lifelong immunosuppression. To this end, insulin secreting 3D cell clusters composed of different types of cells, also referred as heterocellular islet organoids, spheroids, or pseudoislets, have been engineered to overcome the challenges encountered by the current islet transplantation protocols. ß-cells or native islets are accompanied by helper cells, also referred to as accessory cells, to generate a cell cluster that is not only able to accurately secrete insulin in response to glucose, but also superior in terms of other key features (e.g. maintaining a vasculature, longer durability in vivo and not necessitating immunosuppression after transplantation). Over the past decade, numerous 3D cell culture techniques have been integrated to create an engineered heterocellular islet organoid that addresses current obstacles. Here, we first discuss the different cell types used to prepare heterocellular organoids for islet transplantation and their contribution to the organoids design. We then introduce various cell culture techniques that are incorporated to prepare a fully functional and insulin secreting organoids with select features. Finally, we discuss the challenges and present a future outlook for improving clinical outcomes of islet transplantation.

Anti-icing Properties on Surfaces through a Functional Composite: Effect of Ionic Salts.

This study reports the potential of a unique functional composite for anti-icing applications. To date, various ionic salt formulations have been applied to prevent ice accumulation on surfaces. However, salt can be removed by external factors and large amounts must be used to attain anti-icing properties. Incorporating hydrophilic salts into hydrophobic mediums and controlled release of specific agents can provide effective solution to reduce ice accumulation on surfaces. Here, we developed functional polymer composites with salt pockets of altered ionic salts consisting of potassium formate (KCOOH), sodium chloride (NaCl), or magnesium chloride (MgCl2). We dissolved ionic salts in hydrophilic gel domains and dispersed in a hydrophobic styrene-butadiene-styrene polymer matrix. Na+ and Cl- ions delayed ice formation by 42.6 min at -2 °C compared to that for unmodified surfaces. Functional composites prepared with the NaCl ionic salt exhibited better anti-icing behavior at -2 °C because of their high concentration compared to that of the composites prepared with KCOOH and MgCl2 ionic salts. We also characterized the release of ionic salts from composite-modified hydrophobic medium separately up to 118 days. Furthermore, we monitored freezing of water on composite-incorporated or composite-coated hydrophobic surfaces in a camera-integrated cold chamber with a uniform temperature (-2 °C). The results demonstrated significant increases in the delay of freezing on composite-incorporated or composite-coated surfaces compared to that on controls. We observed altered effects of each ionic salt on the mechanical, morphological, and functional properties of the composite-incorporated or composite-coated hydrophobic surfaces. Our results suggested that the efficiency of a polymer composite to promote anti-icing behavior on a surface is directly related to the type and concentration of the particular ionic salt incorporation into the composite. This approach is promising and demonstrates significant potential of the ionic salt embedded within polymer composite-modified hydrophobic surfaces to attain delayed icing function.