[Regularities of endogenous lipid metabolites formation in phorbol 12-miristate 13-acetate-stimulated peripheral blood lymphocytes at leukemia].

Regularities of biologically active lipid metabolites formation in dynamics (5, 10, 30, 60 s) by phorbol 12-miristate 13-acetate stimulation in [14C]palmitic acid have been investigated in normal and leukemia peripheral blood lymphocytes prelabeled with [14C]palmitate. In normal cells there was two-phase formation of 1,2-diacylglycerol (5, 30 s), lysophosphatidylcholine (10, 60 s), as well as free palmitic acid at 10 s of stimulation. Under the identical experimental conditions there was inhibition of investigated lipid release processes at early (5 and 10 s) stages of stimulation of leukemic lymphocytes. At later (30, 60 s) terms of these lymphocytes the activation, basically, similar to norm changes in the formation of palmitic acid-containing metabolites except free palmitic acid (the level of which raised only at 60 second of the post-stimulation) was found. Various protein kinases C are involved in the regulation of investigated lipid levels at certain stages of signal transduction both in norm, and in blast cells. Short-term (5, 10 s) activations of healthy donors lymphocytes are coupled to functioning of Ca2+-independent isoforms of protein kinase C. The inhibition of this protein kinase C in leukemic cells leads to normalization of the investigated lipid release. The data obtained suggests disorders of early membrane-bound reactions in agonist - and a protein kinase C-mediated processes of formation palmitic acid-containing lipid metabolites in the leukemic cells in comparison with the norm.

[The local immune mechanisms of the involvement of the teeth and periodontium in periodic disease]. / O mestnykh immunnykh mekhanizmakh porazheniia zubov i parodonta pri periodicheskoi bolezni.

The aim of our investigations was to elucidate some immune aspects of combination of caries and periodontitis with periodic disease (PD), also known as familial Mediterranean fever. In this regard in patients with active and non-active stage of PD we have studied dynamic changes of concentration of secretory immunoglobulin A (SIgA) in saliva and phagocytic activity of neutrophils derived from gum blood. It has been shown that in patients with PD these indices of local immunity of oral cavity had tendency to a decrease especially in case of PD and periodontitis combination. Disturbances of local immunity was significant in active stage of PD. Based on the obtained data and analysis of latest literature data we suppose that above mentioned changes in local immunity depended on the exhaustion of adaptive properties of patients' local immunity more pronounced in case of chronic inflammation and infection foci formation in oral cavity.