Genomic analysis and biodesulfurization potential of a new carbon-sulfur bond cleaving Tsukamurella sp. 3OW.

Direct combustion of sulfur-enriched liquid fuel oil causes sulfur oxide emission, which is one of the main contributors to air pollution. Biodesulfurization is a promising and eco-friendly method to desulfurize a wide range of thiophenic compounds present in fuel oil. Previously, numerous bacterial strains from genera such as Rhodococcus, Corynebacterium, Gordonia, Nocardia, Mycobacterium, Mycolicibacterium, Paenibacillus, Shewanella, Sphingomonas, Halothiobacillus, and Bacillus have been reported to be capable of desulfurizing model thiophenic compounds or fossil fuels. In the present study, we report a new desulfurizing bacterium, Tsukamurella sp. 3OW, capable of desulfurization of dibenzothiophene through the carbon-sulfur bond cleavage 4S pathway. The bacterium showed a high affinity for the hydrocarbon phase and broad substrate specificity towards various thiophenic compounds. The overall genome-related index analysis revealed that the bacterium is closely related to Tsukamurella paurometabola species. The genomic pool of strain 3OW contains 57 genes related to sulfur metabolism, including the key dszABC genes responsible for dibenzothiophene desulfurization. The DBT-adapted cells of the strain 3OW displayed significant resilience and viability in elevated concentrations of crude oil. The bacterium showed a 19 and 37% reduction in the total sulfur present in crude and diesel oil, respectively. Furthermore, FTIR analysis indicates that the oil's overall chemistry remained unaltered following biodesulfurization. This study implies that Tsukamurella paurometabola species, previously undocumented in the context of biodesulfurization, has good potential for application in the biodesulfurization of petroleum oils.

Genomic analysis provides insights into the Gordonia sp. W3S5 taxonomy and sulfur metabolism-related genes.

The Gordonia sp. W3S5, isolated from oil-polluted soil samples can remove sulfur from a variety of symmetric and asymmetric thiophenic compounds and diesel oil. Its draft genome sequence was comprised of 49 contigs, total genome size 4.86 Mb, and a G + C content of 67.50%. According to the current bacterial taxonomy procedures (16S rRNA gene sequence and overall genome-related index), the W3S5 was affiliated to Gordonia rubripertincta. Rapid Annotation using Subsystem Technology (ClassicRAST) server revealed that the W3S5 contains 4435 coding sequences, 404 subsystems and 60 sulfur metabolism genes. The RAST comparative genomic analysis showed that the genes connected with organic sulfur metabolism are majorly related to ssu and dszABC operons. Moreover, the comparison of orthologous gene clusters using OrthoVenn2 web server revealed a total of 4869 clusters, 2685 core orthologs, 632 shared orthologs and 112 unique ortholog clusters among the W3S5 and other type strains of Gordonia. This is the first report describing genome-based characterization of a Gordonia rubripertincta strain desulfurizing thiophenic compounds and diesel oil. The desulfurization potential of Gordonia rubripertincta W3S5 and genomic analyses revealed it as a valuable biocatalyst for process development to desulfurize a broad range of thiophenic sulfur-containing compounds, which are a major component of organic sulfur in petroleum oil. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1007/s13205-021-02850-4.

Pectins functionalized biomaterials; a new viable approach for biomedical applications: A review.

Pectins are natural complex heteropolysaccharides, composed of (1, 4)-linked α-d-galacturonic acid residues and variety of neutral sugars such as rhamnose, galactose and arabinose. It is second most abundant component of the cell wall of all land plants. It has wide applications in various fields due to its use as gelling, emulsifying or stabilizing agent and as well as its non-toxic, biocompatible and biodegradable nature. Considering these versatile properties this review sheds a light on the synthesis, modification, characterization and applications of pectin based polymers. Most of them are used in industries, pharmaceutics, nutraceutics, drug delivery, tissue engineering, food packaging and cosmetics. Properties of pectin can be improved and modified by forming derivatives, blends and composites.