Effects of melatonin against experimentally induced apical periodontitis in rats.

Apical periodontitis is an inflammatory condition resulting from microbial invasion in the root canal system, causing periapical tissue destruction and bone resorption. This study investigated melatonin's effects, known for its antioxidant and anti-inflammatory properties, on experimentally induced apical periodontitis in rats. Three groups of rats were studied: control, apical periodontitis and apical periodontitis with melatonin treatment. Proinflammatory cytokines and enzyme levels in blood serum were measured, and micro-CT analysis assessed bone resorption. Results showed significantly elevated cytokines and enzyme levels in the apical periodontitis group compared to the control. However, in the melatonin-treated group, these levels were significantly reduced (p < 0.01-0.001). Micro-CT analysis indicated decreased periapical resorption cavity volume and surface area with melatonin treatment. This suggests that systemic melatonin administration can mitigate inflammation and reduce bone resorption in experimentally induced apical periodontitis in rats, potentially holding promise for human endodontic disease treatment pending further research.

Subcutaneous wound infiltration of ketamine is superior to bupivacaine in terms of pain perception and opioid consumption after cesarean section: a double-blinded randomized placebo-controlled clinical trial.

OBJECTIVE: The aim of the present study was to evaluate the analgesic efficiency of SC ketamine, either alone or in combination with bupivacaine, following CS by means of postoperative pain and opioid need. PATIENTS AND METHODS: One hundred and twenty women were allocated into 4 groups in this prospective, double-blind, placebo-controlled, randomized trial. Group K (Ketamine, n=30) received SC 1 mg/kg ketamine. Group B (Bupivacaine, n=30) received SC 20 mL bupivacaine 0.5%. Group KB (Ketamine+Bupivacaine, n=30) received SC ketamine 1 mg/kg plus SC 20 mL bupivacaine 0.5%. Group P (Placebo, n=30) received SC 30 mL 0.9% saline (placebo). RESULTS: VAS scores at resting and on coughing and analgesic consumptions were compared. Visual analogue scale (VAS) pain scores at rest and coughing, at 15 and 60 minutes, and 2, 6 and 12 hours, and total opioid necessity were measured. VAS scores at rest in Group P were higher than in Group KB at the 6th hour, while lower in Group K and Group KB than in Groups B or P at the 12th hour. Patients receiving placebo had higher coughing VAS scores than those receiving ketamine or ketamine+bupivacaine at 2nd, 6th and 12th hours. Patients in Groups P and B required higher doses of morphine than those in groups K or KB. CONCLUSIONS: Subcutaneous ketamine, either alone or in combination with bupivacaine, provides a better postoperative pain relief and reduces postoperative opioid consumption when compared to use of bupivacaine alone.

The comparison of intraincisional bupivacaine infiltration and intravenous paracetamol administration for pain alleviation after cesarean section: a double-blinded randomized placebo controlled clinical trial.

OBJECTIVE: This study aimed to compare the analgesic effect of subcutaneous (SC) bupivacaine and intravenous (IV) paracetamol on postoperative pain and opioid requisites in patients undergoing cesarean delivery. PATIENTS AND METHODS: One hundred and five women were allocated into 3 groups in this prospective, double-blind, placebo-controlled, randomized trial. Group 1 received SC bupivacaine, Group 2 received IV paracetamol following surgery and every 6 hours for 24 hours in the postoperative period, Group 3 received SC 0.9% saline and IV 0.9% saline at similar periods. Visual analogue scale (VAS) pain scores at rest and coughing, at 15 and 60 minutes, and 2, 6 and 12 hours, and total opioid necessity were measured. RESULTS: VAS scores at rest were higher in placebo group than in bupivacaine and paracetamol groups at 15 minutes (p=0.047) and 2 hours (p=0.004). VAS scores at coughing were higher in placebo group than in bupivacaine and paracetamol groups at 2 hour (p=0.001) and 6 hours (p=0.018). Placebo group needed higher (p<0.001) doses of morphine than paracetamol or bupivacaine groups. CONCLUSIONS: Intravenous paracetamol decreases pain scores similar to SC bupivacaine in the postoperative period compared to placebo. Patients taking bupivacaine or paracetamol need fewer opioids than placebo.

Fetal front-abdominal wall thickness in the second trimester as a predictor of abnormal fetal growth.

OBJECTIVES: Worldwide, approximately 9% of infants have a birth weight ≥4000 g, who are defined as fetal macrosomia, with wide variations between countries. Another form of abnormal fetal growth is fetal growth restriction. Infants with low birth weight (LBW) for their gestational age are primarily categorized as either small for gestational age (SGA) or fetal (intrauterine) growth restriction (FGR). All forms of abnormal fetal growth have high morbidity rates of neonatal. Therefore, diagnosis of abnormal fetal growth as early as possible is crucial for optimal clinical care. The measurement of fetal front-abdominal wall thickness (FAWT) is an easy examination. We conducted this study, wondering whether FAWT can predict birth weight or can determine LGA/macrosomia and/or LBW infants in advance. METHODS: This longitudinal cohort study was done in a tertiary center between September 2016 and September 2019. In total, 768 pregnant women with who attended our clinic for oral glucose tolerance test (OGTT) screening between the 26th and 28th weeks of gestation were included in this study. A total of 768 patients were evaluated in the present study. However, 186 of them were excluded in the follow-up of the study because they met the exclusion criteria or they gave birth in another hospital. Eventually, 582 pregnant women were included, with 57 in the LBW group, 461 in the AGA group, and 64 in the LGA group. In addition, 55 fetuses in the LGA group were determined to be macrosomic (birth weight > 4000 g). The FAWT and classic fetal biometric measurements, such as BPD, AC, FL, and EFW, were compared between the AGA group and the macrosomic infants. Statistical analysis was performed to compare the AGA group and the macrosomic infants group. There were no macrosomic infants in the AGA group, so this was used as the control group. RESULTS: There were no significant differences between the groups for maternal age, gravidity, parity, gestational age on the day of the examination, and gestational age at birth. The mean FAWT was significantly thinner in the SGA group than in the appropriate for gestational age (AGA) group (3.4 versus 3.9 mm, respectively, p < .001), while it was significantly thicker in the LGA group than in the AGA group (4.1 versus 3.9 mm, respectively, p < .001). CONCLUSION: FAWT measurement can provide more information and may be more sensitive toward fetal nutrition and growth than the AC value during the second trimester. Some benefits can be gained through the measurement of FAWT at the end of the second trimester. FAWT measurements can be used in obstetrical practice with a similar performance in predicting the LGA and macrosomic infant like AC and EFW.

Digital mammographic interpretation by UK radiographer mammographers: A JAFROC analysis of observer performance.

INTRODUCTION: Radiologists utilise mammography test sets to bench mark their performance against recognised standards. Using a validated test set, this study compares the performance of radiographer readers against previous test results for radiologists. METHODS: Under similar test conditions radiographer readers were given an established test set of 60 mammograms and tasked to identify breast cancer, they were measured against their ability to identify, locate and give a confidence level for cancer being present on a standard set of mammographic images. The results were then compared to previously published results for radiologists for similar or the same test sets. RESULTS: The 10 radiographer readers demonstrated similar results to radiologists and for lesion sensitivity were the highest scoring group. The study group score a sensitivity of 83; a specificity of 69.3 and lesion sensitivity of 74.8 with ROC and JAFROC scores of 0.86 and 0.74 respectively. CONCLUSION: Under test conditions radiographers are able to identify and accurately locate breast cancer in a range of complex mammographic backgrounds. IMPLICATIONS FOR PRACTICE: The study was performed under experimental conditions with results comparable to breast radiologists under similar conditions, translation of these findings into clinical practice will help address access and capacity issues in the timely identification and diagnosis of breast cancer.

Publication trends in micro-CT endodontic research: a bibliometric analysis over a 25-year period.

AIM: To conduct a bibliographic analysis of the research studies published in Endodontics using micro-computed tomography (micro-CT) as an analytical tool over a 25-year period. METHODOLOGY: The Web of Science electronic database was accessed, and an advanced search using strict criteria was undertaken from January 1995 to June 2020 for studies in the field of Endodontics that used micro-CT as an analytical tool. A further search was conducted between January and August of 2020 for a combination of specific terms and descriptors. For each selected article, the following parameters were recorded: field of the study, analysed specimen, publication title and year, authorship, journal of publication, institution and country of origin, collaborating institutions and countries, and number of citations. The acquired data were analysed using descriptive statistics and graphical mappings. RESULTS: The screening process identified 877 relevant articles that were classified into 30 thematic categories. The main fields of research were root canal preparation (23.8%), root canal anatomy (17.4%), canal filling (9.2%) and root canal retreatment (7.0%). The most productive half-decade comprised the period of 2015-2019 (n = 513; 58.5%), with the highest number of articles published in 2019 (n = 146; 16.6%). The authors who lead the list as first authors were G De-Deus, A Keles, F Paqué, MA Versiani and Y Gu, respectively, whilst Versiani was the most productive (n = 51). The leading countries regarding the number of studies were Brazil, China and Turkey with 236, 130 and 65 publications, respectively. The University of São Paulo (n = 90; 10.3%) followed by Wuhan University (n = 37; 4.2%) and the University of Zurich (n = 18; 2.1%) were the prominent contributors by producing the largest number of articles, whilst the University of Zurich was the most-cited institution. The selected studies were published in 140 scientific journals, but the Journal of Endodontics and International Endodontic Journal accounted for almost 50% of the publications. CONCLUSION: This bibliometric analysis portrayed an original and comprehensive view on the progress and trends of the use of micro-CT technology in endodontic research, and enabled a deep understanding of the development process in this field over the last 25 years.

Effects of alpha-lipoic acid therapy on experimentally induced apical periodontitis: a biochemical, histopathological and micro-CT analysis.

AIM: To investigate the possible therapeutic effects of alpha-lipoic acid (ALA) in a model of chronic apical periodontitis in rats by analysing biochemical, histopathological and micro-CT parameters. METHODOLOGY: The study was approved by the Animal Ethics Committee of the Near East University. Thirty-two Wistar rats were divided into four groups of eight rats each: Control Group; ALA Group; AP Group; AP + ALA Group. In the AP and AP + ALA groups, the pulp chambers of the mandibular first molars were surgically exposed and were left open to the oral environment for 4-weeks to allow the establishment of periapical lesions. The rats in the Control and AP groups were treated intraperitoneally with saline solution (with a daily dose of 100 mg kg-1 , for 28 days after periapical lesion induction). The rats in the ALA and AP + ALA groups were treated intraperitoneally with ALA (with a daily dose of 100 mg kg-1 , for 28 days after periapical lesion induction). After decapitation, the trunk blood was collected for the assessment of biochemical parameters. The mandibles were surgically removed and dissected for histopathologic analysis and further scanned with micro-CT. Groups of data were compared with a two-way analysis of variance (two-way anova) followed by Sidak's multiple comparison tests. Values of P < 0.05 were regarded as significant. RESULTS: TNF-α, IL-1ß, MMP-1, MMP-2 levels were significantly lower in AP + ALA group compared with AP group (P < 0.05). There was a significant difference between the AP and AP + ALA groups according to assessment of the inflammatory scores (P < 0.05). The periapical inflammatory infiltrates were significantly more severe (P < 0.05) in the AP group. The AP + ALA group exhibited lower values both in terms of surface area and volume of resorption cavities than the AP group and this difference was significant (P < 0.05). CONCLUSION: alpha-lipoic acid treatment provided therapeutic effects on the inhibition of periapical bone loss.

Interobserver reliability of sonographic fetal biometry in second trimester maternal serum screening.

PURPOSE: To examine the interobserver variability for fetal biometry parameters and to investigate whether this variability affects the second-trimester maternal serum screening test (STMSS) results. MATERIALS AND METHODS: A total of 60 singleton pregnancies who were scheduled for STMSS were investigated. Two experienced sonographers performed all examinations at the same visit. The risk calclations of screening were performed according to the each operator's biometric measurements separately. Interobserver variability in measurements of fetal biometrics and the effect of this interobserver variability on the screening results were assessed. RESULTS: inter-observer reliability for biparietal diameter (BPD) and femur length (FL) were 0.904 and 0.888 (p < 0.00 1), respectively. interobserver reliability coefficients for trisomy 21, trisomy 13/18, and neural tube defect were 0.887, 0.999, and 0.920 (p < 0.0001), respectively. CONCLUSION: The present results demonstrate that the interobserver reliability and agreement of ultrasound measurements of fetal biometry in cases of routine prenatal screening are highly reliable.

Effect of lidocaine spray in pain management during office-based endometrial sampling: A randomised placebo-controlled trial.

Office-based endometrial sampling is the most frequently performed gynaecological procedure. The procedure is usually associated with pain and discomfort. Several anaesthetic and analgesic techniques (e.g., non-steroidal anti-inflammatory drugs, paracervical block, misoprostol and topical anaesthetics) are used for pain management during endometrial sampling. There is no comprehensive study using lidocaine in spray form; we sought to investigate the analgesic efficacy of 10% lidocaine spray in patients undergoing office-based endometrial biopsy. We conducted a prospective, randomised (lidocaine spray (n = 60) and placebo (n = 60), respectively), double-blind study. The mean pain score during procedure was 3.51 ± 1.51 in the lidocaine spray group and 5.11 ± 1.66 in the placebo group. Lidocaine spray treatment significantly lowered the pain scores compared with placebo (p < 0.001). Lidocaine spray can be accepted as a non-invasive, easy to apply and more comfortable anaesthetic method for office-based endometrial sampling.

Nasolacrimal duct intubation in the treatment of congenital nasolacrimal duct obstruction in older children.

PurposeThe purpose of this study was to investigate the outcomes of nasolacrimal duct intubation in the primary treatment of congenital nasolacrimal duct obstruction (CNLDO) in children aged 7 years and older.MethodsThirty children aged ≥7 years who underwent primary unilateral nasolacrimal duct intubation because of CNLDO were enrolled in this study. CNLDO diagnosis was based on signs and symptoms including typical epiphora, increased tear meniscus, recurrent or persistent mucopurulent discharge, and an abnormal dye disappearance test (DDT). Surgical success was defined as complete resolution of previous signs and symptoms and DDT grade 0-1.ResultsThe mean age was 10.7±2.5 years (ranging from 7 to 15 years). Of the 30 patients, 18 were male and 12 were female. The mean follow-up period was 8.8±3.4 months (ranging from 6 to 16 months). The mean silicone tube removal time was 4.6±1.1 months (ranging from 3 to 6 months). The complete resolution of signs and symptoms with DDT grade 0-1 was observed in 22 of 30 cases (73.3%) during the follow-up period. The mean age of the patients with unsuccessful outcomes was 12.7±1.4 years, whereas that of the patients with successful outcomes was 10.0±2.4 years, and the difference was statistically significant (P=0.006). No serious intra- and/or post-operative complication was observed.ConclusionsThe current study demonstrates that nasolacrimal duct silicone intubation with intranasal endoscopic visualization has favorable outcomes as a primary treatment of persistent CNLDO in children aged 7 years and older. It can be used to reduce the need for dacryocystorhinostomy which is a more invasive procedure.

Detection of Toxoplasma gondii and Cyclospora cayetanensis in Mytilus galloprovincialis from Izmir Province coast (Turkey) by Real Time PCR/High-Resolution Melting analysis (HRM).

To evaluate the presence of Toxoplasma gondii and Cyclospora cayetanensis in edible shellfish, a total of 795 specimens of Mytilus galloprovincialis from 8 different sites on the west coast of Turkey (Bays of Izmir and Mersin) were examined. Fifty-three pooled samples were created and subjected to EvaGreen(®) RealTime PCR assay and HRM analysis for protozoans detection and oocyst quantification. Seven out of 8 sites were contaminated with at least one protozoan species. Twenty-one out of 53 (39.6%) samples tested positive for T. gondii, C. cayetanensis or both pathogens: 26.4% samples positive for C. cayetanensis; 9.4% for T. gondii, and 3.8% for both protozoans. The number of oocysts in positive samples ranged from 6 to 30 for T. gondii and from 3 to 48 for C. cayetanensis. This is the first report of T. gondii Type 1, and C. cayetanensis in M. galloprovincialis, the most consumed shellfish species in Turkey.

Effect of yearly conditions and management practices on ochratoxin A production in Sultana Seedless vineyards.

Sun drying of seedless grapes (Vitis vinifera cv. Sultanina) is widely practised in the western Aegean Region providing Turkey with a significant share in the world trade of sultanas. Research was initiated in 1998 to determine the incidence of ochratoxin A (OTA), to identify the major factors resulting in contamination and to develop techniques to reduce or prevent contamination. This paper assesses OTA formation in five experimental vineyards located in Manisa province of Turkey between 1998 and 2003. The cultural practices recorded were tillage (type and timing), fertilisation, plant protection, irrigation, trellising, pruning, GA(3) application, harvest maturity and date, and drying practices (type of drying yard, dipping into alkaline solution, length of drying period). In the experimental vineyards, no OTA was found at veraison. OTA levels in grapes harvested at fresh maturity ranged between

Evaluation of the mental foramen and accessory mental foramen in Turkish patients using cone-beam computed tomography images reconstructed from a volumetric rendering program.

This study determined the occurrence and location of the mental foramen (MF) and accessory mental foramen (AMF) in Turkish patients using cone-beam computed-tomography (CBCT) with 3D-imaging software. CBCT scans of 386 sites in 193 (92 male, 101 female) patients were retrospectively analyzed to determine MF and AMF occurrence, sizes, and locations. Digital imaging and communications in medicine (DICOM) data were transferred to surface-rendering software to generate 3D images. Distances between the MF and AMF and from both foramina to the alveolar ridge and to the closest tooth were measured. Differences in AMF incidence by sex, side, and location were evaluated using chi-squared tests, and MF and AMF measurements were evaluated using Mann-Whitney U-tests. AMFs were observed in 6.5% of patients and were most commonly in an anteroinferior location. Mean AMF size did not differ significantly by sex or side [males: horizontal = 1.5 mm (1.0-2.4 mm), vertical = 1.4 mm (0.8-2.4 mm); females: horizontal = 1.5 mm (0.8-3 mm), vertical = 1.3 mm (0.8-2.1 mm); P > 0.05]. Males showed significantly greater mean vertical and horizontal MF dimensions compared with females [males: horizontal = 3.9 mm (1.0-7.0 mm), vertical = 3.6 mm (1.2-7.0 mm); females: horizontal = 3.5 mm (1.3-5.6 mm), vertical = 3.3 mm (0.8-5.8 mm); P < 0.05]. Awareness of the AMF is important to avoid mental nerve damage during surgical intervention and anesthetic applications. CBCT is useful for AMF detection, distributes less ionizing radiation, and allows 3D imaging.

Fumonisin B1 and B2 occurrence in dried fig fruits (Ficus carica L.) under Meander Valley's climatic conditions and relationship with fruit quality.

Fusarium is the agent causing endosepsis (internal rot) in fig fruits and it is widespread in fig orchards in the Aegean region. This research was conducted to determine the natural occurrence of fumonisin B(1) (FB(1)) and B(2) (FB(2)) on dried fig fruits of Sarilop (syn. Calimyrna) variety which are mainly grown in the Big and Small Meander Basins in the Aegean region, representing 60% of world dried fig production. A total of 262 samples belonging to two quality classes, Class A and Class cull, were collected from 12 different locations during the two crop years in 2004 and 2005. The fumonisin detection method is based on extraction with methanol-acetonitrile-water, derivatization with o-phthaldehyde and quantification by reversed-phase high-performance liquid chromatography with fluorescence detection. The mean concentrations of FB(1) and FB(2) in fumonisin-positive samples were 0.080 ± 0.047 µg g(-1) and 0.055 ± 0.031 µg g(-1) and ranged from LOD to 0.332 µg g(-1) and from LOD to 0.198 µg g(-1), respectively. The incidence of fumonisins significantly differed between the two crop years. This difference can be attributed to the alteration in the rainfall regime from mid-May to mid-August (7.2 mm in 2004, 90.9 mm in 2005) and number of humid wind currents from a westerly direction (183 in 2004, 492 in 2005) from the end of July and mid-August that may have triggered a higher incidence of Fusarium spp. and thus fumonisin production.

Five-year survey of ochratoxin A in processed sultanas from Turkey.

The results of surveillance for ochratoxin A (OTA) in 1885 samples of sultanas taken during five crop years between 1999 and 2003 are reported. The analytical method was based on extraction with methanol + sodium bicarbonate and clean-up by immunoaffinity column chromatography followed by high-performance liquid chromatography with fluorescence detection. The limit of detection for OTA was 0.3 microg kg(-1). The results show that 9.3% of the samples contained no detectable levels of OTA, whereas 0.6% had concentrations exceeding 10 microg kg(-1); the remaining 90.3% had levels within the range 0.3-10 microg kg(-1). The overall mean OTA concentration in the total number of 1885 samples taken was 1.36 +/- 2.91 microg kg(-1); the overall median was calculated as 0.90 microg kg(-1).

Comparative evaluation of western blotting in hepatic and pulmonary cystic echinococcosis.

Many serological tests are widely used in the diagnosis of cystic echinococcosis (CE), caused by the larval stages of Echinococcus granulosus. The present study was carried for differentiation between hepatic and pulmonary cystic echinococcosis by Western Blotting (WB). A total of 121 sera from patients with hepatic CE (37), pulmonary CE (31) and controls (53; consisting of six healthy, seven Hymenolepis nana infection, 20 hepatic and 20 pulmonary diseases other than CE) were examined. In all of the CE patients, E. gronulosus infection was confirmed by surgical intervention. Sera were previously tested using IHA and ELISA to detect the E. gronulosus specific antibodies. Sera from hepatic cases of CE reacted with 16 polypeptides of 6-116 kDa and sera from pulmonary cases of CE reacted with 14 polypeptides of 4-130 kDa by Western Blotting. The WB test enabled the detection of antibodies in the hepatic CE samples for proteins of 24, 32 34, 44-46 and 52-54 kDa in molecular weight in 78.4%, 75.7%, 78.4% and 89.2% of the patients, respectively. In the pulmonary CE samples sera WB test enabled the detection of antibodies 24, 44-46, 100, 110, 116 and 120 124 kDa in molecular weight in 81.3%, 75.0%, 87.5%, 71.9%, 84.4% and 65.6% of the patients, respectively. We indicated that the antigenic components of high molecular weight can be good candidates for differentiation of hepatic CE from pulmonary CE.

Ochratoxin A in sultanas from Turkey I: Survey of unprocessed sultanas from vineyards and packing-houses.

A method for the determination of ochratoxin A (OTA) in sultanas from Turkey using extraction with a sodium bicarbonate solution (2% NaHCO3) followed by immunoaffinity clean-up and liquid chromatography with fluorescence detection was used to assess the frequency of occurrence and level of OTA. In-house validation was carried out with spiked samples at levels of 0.15, 1.5, 5.0 and 10 microg kg-1 and average recoveries were 91, 93, 87 and 89%, respectively. The limits of detection and limit of quantification in Turkish sultanas were 0.026 and 0.09 microg kg-1, respectively. A survey for the presence of OTA was carried out on 264 unprocessed sultana samples during the production seasons between 1998 and 2000 collected annually from vineyards and from packing-houses. The analyses of unprocessed sultanas showed that 32.2% of the total number of samples contained no detectable OTA, whereas 9.8% of sultana samples had OTA concentrations above 10 microg kg-1, and the remaining 58% had levels within the range 0.026-10 microg kg-1. There were big differences in median concentrations between years. Considering the year of production, it appears that sultanas produced in 1998 and 2000 showed the lowest incidence of OTA contamination (median<0.02 microg kg-1), whereas 2002 showed the highest incidence (median=4.3 microg kg-1). The overall mean OTA concentration was calculated as 3.4 microg kg-1, and the overall median as 0.9 microg kg-1. Among the samples analysed, the highest detected level of OTA was 54 microg kg-1.

Toxoplasmosis in last four years in Agean region, Turkey.

Anti-Toxoplasma antibodies were determined with IFA and ELISA tests in 9410 patients who were different age groups, attended Department of Parasitology between 1991-1995. Anti-Toxoplasma IgG antibodies were found positive in 4651 (49.4%) of these patients. 2287 (21.4%) patients were pregnant women and the positivity was 55% of them. According to history of these patients, seropositivity was found 50% in women having spontaneous abortion, 52% in women having stillbirth, in 55% women having abnormal fetal births. These patients and their culinary habits, the presence of cats and relationship with other clinical symptoms were also evaluated.