Boron-incorporated biocomposite coatings on 316L and NiTi alloys: Enhanced structural, antibacterial activity, and cell viability performances.

Boron doped (5 %, 10%, and 15 wt.%) Hydroxyapatite (B-HA) biocomposites were syntesized and coated on 316L SS and NiTi (Ni-45Ti) metallic substrates by using the electrophoretic deposition process (EPD). The morphological and structural characterization of the coatings was executed using scanning electron microscopy (SEM) and X-ray diffraction devices (XRD). Antibacterial tests were conducted using Escherichia coli (E. coli, JM103) and Staphylococcus aureus (S. aureus, ATCC29293) microorganisms. The mitochondrial activity assay (MTT)-[3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] was used to examine cell viability and cytotoxicity in Saos-2 osteoblast cells. HA and boron peaks, as well as B-TCP and metallic components, were detected in XRD examinations. Porous morphologies were generated on the surface with boron doped B-HA coatings, as revealed by SEM views. Antibacterial activity studies revealed that both metallic coating groups, notably with boron doping, demonstrated antibacterial activity against gram-negative E. coli and gram-positive S. aureus. The antibacterial activity of the 316L group was shown to be better than that of the NiTi group in comparisonal testing. The syntesized boron-doped biocomposite coatings did not have any detrimental effects on living cells, according to cell viability studies. The cell viability rate was found to be greater in NiTi coatings than in 316 SS coatings, and the impact was amplified by the addition of boron.

The attitudes of medical students to autopsy.

To assess the attitudes of medical students to autopsy, 6th year students in the Medical Schools located in Istanbul, Edirne and Bursa in Turkey were asked to participate in a questionnaire. In the questionnaire, the main questions were on the number of autopsies they had attended, the number of autopsies they had participated in, their reaction to the first autopsy, whether they felt the conditions were adequate or not and what would their objectives be upon deciding to perform an autopsy. In the evaluation, it was detected that most of the participants had attended an autopsy, only a minority had participated in autopsy and most of them felt uncomfortable and inadequate on performing autopsy. The results were discussed with respect to the literature.

Medico-legal death investigations and autopsies in Istanbul, Turkey.

There are two different organizations in forensic medicine in Turkey: the universities and the Council of Forensic Medicine (CFM). The CFM is an official body of the Ministry of Justice and serves as an expert witness on technical and medical subjects. The basic duty of the universities is education in the medical schools with the cooperation of the CFM. This study examines 3064 medico-legal death investigations and 2548 autopsies performed in the CFM, Ministry of Justice, Istanbul and in the eleven units of CFM in Istanbul during 1996. The age and sex distributions, the incidence and causes of deaths were reviewed.