Synthesis and biological evaluation of novel 2 (4`-hydroxynaphthyl) chromen-4-one as a CK2 inhibitor.

Protein kinase CK2 is a potential drug target for many diseases including cancer, inflammatory disorders, Alzheimer's disease, Parkinson's disease and viral infections. Significant efforts have been made for the discovery of potent inhibitors of this enzyme. Herein, we report on the synthesis, characterization, and biological evaluation of novel flavonoid compounds as CK2 inhibitors. The tested compounds were 2 (4`-hydroxynaphthyl) chromen-4-one which is a naphthyl backbone flavonoid with an IC50 value of 0.45±0.059 µM and 2(4-hydroxyphenyl)-4H-chromen-4-one a phenyl based derivative with an IC50 value of 0.33±0.048 µM. Cell viability was tested using MCF-7 cells. Both compounds were able to reduce the cell viability around 50 % in concentration of 100 µM after 48 h. Molecular modeling studies were performed to understand the binding mode of both compounds.

Laparoscopic-assisted management of severe necrotizing pancreatitis with obstructive jaundice: a case report.

We report a severely infected necrotizing pancreatitis managed with hand-assisted laparoscopic necrosectomy along with a review of the relevant literature. Minimally invasive necrosectomy has been shown to be efficient and advantageous in managing necrotizing pancreatitis. Multiple techniques have been advocated over the last decade. Laparoscopic pancreatic debridement is a feasible option for some patients with necrotizing pancreatitis. We selected hand-assisted laparoscopic pancreatic necrosectomy, which has gained some favor over open necrosectomy because of the morbidity and mortality associated with laparotomy. We report on an Indian male patient who presented with acute abdomen and severe jaundice. A CT scan of the abdomen showed severe necrotizing pancreatitis. After conservative management failed, a hand-assisted laparoscopic pancreatic necrosectomy was performed. The patient recovered and was discharged 4 weeks after surgery.

Neutral carriers based polymeric membrane electrodes for selective determination of mercury (II).

The potentiometric response characteristics of mercury ion-selective membrane electrodes based on 2-amino-6-purinethiol (I(1)) and 5-amino-1, 3, 4-thiadiazole-2-thiol (I(2)) were described. Ion selectivities were tested for various plasticizers, which were used as solvent mediators to incorporate the ionophores into the membrane. Effects of experimental parameters such as membrane composition, nature and amount of plasticizers and additives, pH and concentration of internal solution on the potential response of Hg(2+) electrodes were investigated. The best performance was obtained with the electrode having a membrane composition (w/w) of (I(1)) (3.17%): PVC (31.7%): DOP (dioctylpthalate) (63.4%): NaTPB (sodium tetraphenylborate) (1.58%). The proposed electrode reveals a Nernstian response over Hg(2+) ion in the concentration range of 7.0 x 10(-8)-1.0 x 10(-1) M with limit of detection 4.4 x 10(-8) M. The electrode shows good discrimination toward Hg(2+) ion with respect to most common cations. It shows a short response time (10s) for whole concentration range and can be used for 2 months without any considerable divergence in potentials. For evaluation of the analytical applicability, the electrode was used in the determination of Hg(2+) ion in different environmental and biological samples. The practical utility of the membrane electrode has also been observed in the presence of surfactants.

A new Zn(II)-selective potentiometric sensor based on 4-tert-butylcalix[4]arene in PVC matrix.

Poly(vinyl chloride) (PVC) based membranes containing 4-tert-butylcalix[4]arene (I) as an electroactive material along with anion excluder sodiumtetraphenylborate (NaTPB) and plasticizer tri-butylphosphate (TBP) have been developed to fabricate a new zinc-selective sensor. Out of various compositions, the best performance was exhibited by the membrane having I, NaTPB, TBP and PVC in the ratio 8:5:100:200 (w/w). The sensor works well in the concentration range 9.8 x 10(-6) to 1.0 x 10(-1) mol dm(-3) with a near-Nernstian slope of 28.0+/-1.0 mV/decade of activity. The detection limit is down to 5.0 x 10(-7) mol dm(-3). The working pH range of this sensor is 2.5-4.3 and it works well in partially non-aqueous medium up to 15% (v/v) (methanol, ethanol and acetone). It exhibits a fast response time of 30s and could be used for more than four months without any considerable change in response characteristics. It has excellent selectivity for Zn(II) over other mono-, bi- and trivalent cations which have been reported to cause interference in the working of other sensors. It has been successfully used as an indicator electrode in the potentiometric titration of Zn(II) against EDTA and also to estimate zinc ions in industrial waste waters.

Nutritional support in patients with low volume chylous fistula following radical neck dissection.

One of the well known complications of radical neck dissection is a chylous fistula, which results from injury to the thoracic duct as it enters the left subclavian vein. Such fistulae may cause considerable increased morbidity to a patient who is already debilitated by malignancy and by the increased catabolic response to surgery. Further surgery may be appropriate for those with a high fistula output but conservative therapy is normally advocated for the remainder. Nutritional and electrolyte support for these patients is essential and poses potential problems in management. We present three such patients. One was fed parenterally and two enterally and in all cases the fistulae closed spontaneously. We examine the known physiological stimuli to chyle production and conclude that the enteral feedings of these patients with fat or an isomolar enteral feed does not, contrary to current belief, increase chyle flow or delay the healing of these fistulae.

Midline sublingual dermoid cyst.

The case of an 18-year-old man who presented with a large cystic mass in the floor of the mouth extending into the left submandibular triangle of the neck is reported. Histopathology of this mass, which was totally excised by a combined intra-oral and cervical approach, confirmed it to have originated in ectodermal remnants (congenital dermoid cyst) and to be actively secreting sebum. This case is reported both because dermoids at this site are rare because they may present some problems in management.

[Kinetics of the temperature dependence of thermal activation and inactivation of bacterial endospores as a consequential reaction]. / Zur Kinetik der Temperaturabhängigkeit der Hitzeaktivierung und -inaktivierung von Bakterienendosporen als Folgereaktion.

Reaction kinetics explains the thermal activation and destruction of bacterial endospores as a consequent reaction with the activation rate constant k 1 of the resting spores N 0 and the destruction rate constant k 2 of the heat-activated spores N ++: (formula see text). If one takes also into account the activated spores already present in a suspension, one obtains, at constant temperature, destruction curves that become straight lines when plotted semi-logarithmically or correspond to the convex and concave curves often described in the literature. The D value of destruction must be calculated from the k value of the slowest reaction. Experiments with endospores of Bac. subtilis showed that k 1 greater than k 2 at temperatures less than 100 degrees C, and k 2 greater than k 1 at temperatures greater than 135 degrees C. In case of further confirmation of this theory, far-reaching conclusions might be drawn as to the calculation of the sterilization of F value.