Evaluation of Heavy Metal and Specific Trace Elements Levels Among Fast-Food Workers and Their Susceptibility to Atherosclerosis.

Fast-food workers in Iraq face significant health risks due to exposure to heavy metals from fumes and dust during cooking activities. Heavy metals, such as lead (Pb), cadmium (Cd), and nickel (Ni), are toxic to cells even at low concentrations and can cause health risks, including atherosclerosis, due to oxidative stress and reduced antioxidant activity. To the best of our knowledge, this is the first study assess the levels of heavy metals in fast-food workers and investigate their potential link to atherosclerosis development by monitoring the levels of copper (Cu), zinc (Zn), magnesium (Mg), manganese (Mn), and iron (Fe). A total of 120 male participants aged between 20 and 40 years were included in the study, with 40 fast-food workers, 40 patients with atherosclerosis, and 40 healthy individuals evaluated. The levels of Pb, Cd, Ni, Cu, Zn, Mg, Mn, and Fe in all blood samples were determined using atomic absorption spectrometry. Results showed that the fast-food worker group had significantly higher levels of Pb, Cd, Cu, and Fe compared to the healthy control group, with increases of 57%, 75%, 30%, and 55%, respectively. Conversely, their levels of Zn and Mg were significantly lower, decreasing by 15% and 16%, respectively. On the other hand, the atherosclerosis patients' group had significantly higher levels of Pb, Cd, Cu, and Fe, with increases of 47%, 74%, 34%, and 28%, respectively, as well as significantly lower levels of Zn and Mg, decreasing by 17% and 21%, respectively, compared to the control group. These findings suggest that fast-food workers are at risk of developing atherosclerosis due to exposure to high levels of heavy metals and imbalances in essential trace elements. The results showed a significant increase in the levels of Pb and Cd in the sera of these workers, which was expected because of the long duration and high intensity of exposure to toxic heavy metals. This is a serious indicator that must be considered, as it has been previously established that increased levels of Pb and Cd in the body are linked to the risk of atherosclerosis. Additionally, an association between Pb and Cd levels and an imbalance in trace element levels (Cu, Zn, Mg, and Fe) were observed. The Implementation of stricter regulations and guidelines for maintaining cleanliness and safety in fast-food restaurants may be crucial for protecting workers and preventing long-term health complications.

A comparative study of serum Zn, Cu, Mg, Mn, Cr, and Fe levels and their association with the vulnerability of Iraqi COVID-19 patients.

BACKGROUND: For the immune system to protect the body from infectious diseases such as COVID-19, it needs the ideal amount of vital trace elements. Trace element levels, especially, zinc (Zn), copper (Cu), magnesium (Mg), manganese (Mn), chromium (Cr), and iron (Fe) levels, may affect how sensitive an individual is to COVID-19 and other viruses. The current study evaluated the level of those trace elements during stays in the isolation center and investigated their association with vulnerability to COVID-19. METHODS: A total of 120 individuals, 49 males and 71 females aged between 20 and 60 years, were included in this study. Forty individuals infected with COVID-19, 40 individuals who had recovered from it, and 40 healthy individuals, were all evaluated and studied. By using a flame atomic absorption spectrophotometer, levels of Zn, Cu, and Mg were assessed for all samples, whereas levels of Mn, and Cr were determined by a flameless atomic absorption spectrophotometer. RESULTS: The infected individuals had significantly lower levels of Zn, Mg, Mn, Cr, and Fe than recovered individuals and healthy control individuals (P < 0.0001). On the other hand, the total number of infected patients was found to have much higher levels of Cu than those in the recovered group and the control group. For the recovered and healthy control groups, no significant differences were observed in the levels of trace elements (P > 0.05), except for Zn (P < 0.01). Also, the findings indicated no association of trace elements with age and BMI (P > 0.05). CONCLUSION: These results show that an imbalance in the levels of essential trace elements could be associated with increasing the risk of COVID-19 infection. However, additional thorough research of greater scope is required considering the severity of the infection.

Evaluation of some trace elements and antioxidants in sera of patients with rheumatoid arthritis: a case-control study.

INTRODUCTION: Rheumatoid arthritis (RA) is a chronic autoimmune inflammatory disease that destroys joints. The concentrations of elements (Zn, Cu, Mg, Mn, Fe, Ni, Cr, K, Na, Ca, Pb, and Cd), as well as antioxidant glutathione GSH and lipid peroxidation marker malondialdehyde MDA, will be determined in this study. METHOD: The study involves 120 female RA patients and 60 healthy females in the control group, all of whom are between the ages of 20 and 60. Patients having diabetes, hypertension, hyperthyroidism, or psoriasis, in addition to RA, were excluded. RESULTS: When RA patients were compared to healthy individuals, the levels of Cu, Ni, Na, Pb, and Cd were significantly higher (p < 0.01), whereas the levels of Zn, Mg, Mn, Cr, Ca, K, and Fe were significantly lower (p < 0.01). The Cu/Zn (2.21 and 1.16) and Na/k (38.67 and 34.87) ratios were also found to be significantly higher in RA patients compared to controls. Furthermore, the data indicated that GSH concentrations in RA patients were significantly lower (p < 0.01) than those in controls (655.90 and 1345.17 mol/l, respectively). MDA levels in RA patients were significantly higher (p < 0.01) than those in controls (2.739 and 1.673 mol/l, respectively). CONCLUSIONS: The data indicate that Cu/Zn and Na/k ratios can be of great value in the diagnosis and evaluation of RA patients. On this basis, we conclude that these elements can be relied upon to provide indications of RA risk and the measurement of oxidative stress can serve as a biomarker to monitor disease activity and severity in RA patients. Key Points • Trace elements may be of value and may have an expected significance in the early diagnosis, prognosis, and treatment evaluation of rheumatoid arthritis. • The distortion in the levels of trace elements may reflect their possible role in the pathogenesis of rheumatoid arthritis. • Oxidative stress can act as a biomarker to monitor disease activity and severity in patients with rheumatoid arthritis. • The trace elements can contribute to the manifestation of inflammatory diseases due to their role in the synthesis of some antioxidants or due to their effect on the immune system.

Evaluation of some trace elements in sera of asthma patients: a case control study.

INTRODUCTION: Asthma is a chronic inflammatory disease of the airways characterized by recurrent respiratory symptoms of dyspnea, wheezing, chest tightness, and cough. AIM: This study aims to investigate the concentrations of Cu, Zn, Mg, Mn, Fe, Cr, Ni, and Al in the serum of asthmatic patients. MATERIALS AND METHODS: An atomic absorption technique was used to determine the levels of trace elements. The study included sixty asthmatic patients and ninety healthy individuals as control group their ages ranging from 20 to 45 years. RESULTS: We found a significant increase of the levels of copper, iron, and aluminum by 20%, 54%, and 47% (p<0.01), respectively, in the asthmatic patients as compared with the controls. On the other hand, in comparison with the controls, the levels of zinc, magnesium, manganese, and nickel were found to be significantly decreased (p<0.01) by 24%, 16%, 53%, and 81%, respectively, in the asthmatic patient group. Moreover, chromium level showed non-significant differences (p>0.05) between patients and control group. CONCLUSIONS: The increased level of Cu and Fe may reflect their potential role in the pathogenesis of asthma. Furthermore, the serum iron levels tend to be increased as a result of the inflammatory process that occurs in asthma. Low levels of Zn, Mg, and Mn may contribute to allergic diseases due to their role in the synthesis of certain antioxidants or to their effect on the immune system.

Evaluation of Some Trace Elements and Vitamins in Major Depressive Disorder Patients: a Case-Control Study.

Major depressive disorder (MDD) is a common mental disorder worldwide; however, little is known about its etiology. It is well known that levels of certain trace elements are associated with the pathogenesis of some diseases. Accordingly, this study aims to evaluate the effect of trace elements and vitamins in the etiology of MDD. In this case-control study, sixty men patients with MDD and sixty, age and gender matched, control subjects were examined. Serum levels of Cu, Zn, Ni, Cr, Mn, Mg, and Al were determined by atomic absorption spectrometry as well as serum levels of vitamins E and A were determined using high-performance liquid chromatography. The results revealed that there were significantly higher levels (p < 0.001) of Cu, Cr, and Al in patients sera compared with control. While there were significantly lower levels (p < 0.001) of Zn, Ni, Mn, Mg, vitamin E, and vitamin A in MDD patients as compared with control. In addition, high Cu/Zn ratio (p < 0.05) was observed with the depressive disorder patients. The present study highlights some main indications: a significant relationship between the disturbances of element levels and vitamins (E and A) with MDD. Cu and Zn seemed to have a crucial role in understanding the pathogenesis of depressive disorders, where Cu/Zn ratio could have an important role in the diagnosis and monitoring of MDD. Moreover, the results suggest that the reduction in the antioxidant vitamin E leads to increased risk of MDD. Finally, more studies on using trace element supplementation would be suggested to clarify their effect, in order to improve the therapy of MDD.