[Antibioresistance of Escherichia coli strains isolated from raw chicken meat in Senegal]. / Resistance aux antibiotiques des souches d'Escherichia coli isolees de la viande de poulet de chair au Senegal.

INTRODUCTION: Antimicrobial-resistant Escherichia coli and others pathogens bacteria can be transferred from animals to humans through consumption of contaminated food and foods products and thus present a public health risk. The increase in E. coli resistance to commonly used antimicrobials both in the public health and veterinary sectors is one of the major threats of health care worldwide. The present study was undertaken to estimate the antimicrobial resistance of E. coli isolates from raw chicken meat in Dakar. MATERIAL AND METHOD: Levying of skin and muscle have been carried out on 120 chicken carcasses bought from 13 sale points and 23 flocks beetween November 2003 and April 2004. 102 Escherichia coli strains have been isolated, among which, 90 were tested for their susceptibilities to 16 selected antibiotics by agar diffusion method. RESULTS: All Escherichia coli strains (100%), were resistant to one or more antibiotic; 60 strains (66.66%) being resistant to more than five antibiotics. Those frequently encountererd are: ampicillin, trimethoprim, trimethoprim-sulfametoxazole, tetracycline, sulfonamides, streptomycin, nalidixic acid. Multiple resistances to 12 antibiotics were also observed. The lowest resistances were noted with gentamicin (3.33%) and neomycin (5.56%). CONCLUSION: This study showed the significance of chicken meat as source of Escherichia coli strains with a simple or multiple resistance to various antibiotics tested. Further studies are necessary in order to determine bacterium mechanisms of resistance.

[Antibiotic resistance of Escherichia coli strains isolated from raw chicken meat in Senegal]. / Resistance aux antibiotiques des souches d'Escherichia coli isolees de la viande de poulet de chair au Senegal.

INTRODUCTION: Antimicrobial-resistant Escherichia coli and others pathogens bacteria can be transferred from animals to humans through consumption of contaminated food and foods products and thus present a public health risk. The increase in E. coli resistance to commonly used antimicrobials both in the public health and veterinary sectors is one of the major threats of health care worldwide. The present study was undertaken to estimate the antimicrobial resistance of E. coli isolates from raw chicken meat in Dakar. MATERIAL AND METHOD: Levying of skin and muscle have been carried out on 120 chicken carcasses bought from 13 sale points and 23 flocks beetween November 2003 and April 2004. 102 Escherichia coli strains have been isolated, among which, 90 were tested for their susceptibilities to 16 selected antibiotics by agar diffusion method. RESULTS: All Escherichia coli strains (100%), were resistant to one or more antibiotic; 60 strains (66.66%) being resistant to more than five antibiotics. Those frequently encountererd are: ampicillin, trimethoprim, trimethoprim-sulfametoxazole, tetracycline, sulfonamides, streptomycin, nalidixic acid. Multiple resistances to 12 antibiotics were also observed. The lowest resistances were noted with gentamicin (3.33%) and neomycin (5.56%). CONCLUSION: This study showed the significance of chicken meat as source of Escherichia coli strains with a simple or multiple resistance to various antibiotics tested. Further studies are necessary in order to determine bacterium mechanisms of resistance.

[Comparison of two polysaccharide antigens for the serological diagnosis by ELISA of porcine pleuropneumonia (serotype 5)]. / Comparaison de deux antigènes de nature polysaccharidique pour le diagnostic sérologique par ELISA de la pleuropneumonie porcine (sérotype 5).

Two antigens of polysaccharidic nature were evaluated for their specificity in serodiagnosis of porcine pleuropneumonia (serotype 5). Recently, long chains lipopolysaccharides (LC-LPS) was shown to increase the specificity of the ELISA test for A. pleuropneumoniae serotype 5 compared to a saline extract of boiled-formalinized whole cells. Nevertheless, some cross reactions were noticed with LC-LPS. We have thus cleaved the LPS molecule at the ketosidic bond between the lipid A and the core. The lipid A was separated from the polysaccharide (PS) by centrifugation. With an indirect sandwich ELISA, it was possible to demonstrate that the PS yielded positive responses with homologous sera (serotype 5) and negative responses with heterologous sera (serotype 3 and undeterminated serotype). Overall, the use of the PS did not notably increase the specificity of the ELISA test. Since production of PS from LC-LPS and its separation from the lipid A is not simple, we do not recommend its use in serodiagnosis.

[Socio-ethnology and role of the dog in the Pikine district (suburban zone of Dakar - Senegal)]. / Socio-ethnologie et rôle du chien dans le département de Pikine (zone suburbaine de Dakar-Sénégal).

During a mass campaign in 1987, the authors vaccinated about 500 dogs in the Pikine district. The dogs were kept for security reasons. Religion was not a limiting factor to dog ownership, but ethnic influences meant that those from Casamance (Manjaks, Diolas,. . . )--although a minority meant that among the population--bred more dogs than the Wolof majority. Most of the dog population is of local and young (average age 29 months). Males are preferred over females. The dogs belong to youths or adolescents who are without means and can neither feed the dogs correctly nor look after them when they fall sick. This individual poverty explains why the dogs are not vaccinated and why they scavenge for survival, thus contacting permanent strays, walking real reservoirs of rabies virus! The affection felt by young people for dogs makes them choice targets for transmission of the virus to man.