[Creatine kinase BB and lactate in the cerebrospinal fluid of neonates and infants with perinatal injuries of the CNS]. / Kreatinkinaza-BB i laktat v spinnomozgovoi zhidkosti u novorozhdennyki u detei rannego vozrasta s perinatal'nymi porazheniiami TsNS.

A study was made of the content of creatine kinase-BB (CK-BB) and lactate in cerebrospinal fluid (CSF) of 202 neonates and infants with perinatal CNS injuries. The relationship was found between the rise of the CK-BB content and the gravity of perinatal CNS injuries. The highest content of CK-BB in CSF was marked in neonates with cerebral disorders complicated by infectious and inflammatory diseases (pneumonia, sepsis). Within the first 5 days of life, the children of this group demonstrated the relationship between the content of CK-BB and lactate of CSF. The measurement of the content of CK-BB in CSF should be used for early diagnosis, assessment of the gravity and course of perinatal CNS injuries in neonates and in infants.

[Efficiency and mechanisms of antihypoxic effect of ATP in newborn rats]. / K voprosu ob éffektivnosti i mekhanizmakh protivogipoksicheskogo deistviia ATF na organizm novorozhdennykh krysiat.

Effect of ATP (administered at the doses of 20 mg and 100 mg per kg of body mass) on sensitivity of 5-7 days old rats to acute hypoxia as well as content of 2,3-DPH and ATP in erythrocytes were studied. ATP exhibited the most effective antihypoxic influence at a dose of 100 mg/kg. After administration of ATP a dissimilar increase in content of 2,3-DPH and ATP was observed in erythrocytes of newborn rats maintained under conditions of normal atmospheric pressure. At the hypoxic step of compensated metabolic acidosis of blood the increase in content of 2,3-DPH, caused by the ATP administration, was combined with a less distinct alterations in acid-alkaline balance (SB, BE). Under conditions of decompensated metabolic acidosis preadministration of ATP did not prevent the pH dependent decrease in 2,3-DPH content in erythrocytes but slightly increased the ATP content. The alterations in content of 2,3-DPH and ATP, which occurred in erythrocytes apparently due to the effect of products of ATP dephosphorylation and which improved the oxygen transport by hemoglobin under conditions of oxygen deficiency, might be responsible for the positive influence of ATP on the stability of newborn rats to acute hypoxia.

[Characteristics of the molecular diversities of human and rat aldolase via starch and agar gel electrophoresis]. / Kharakteristika molekuliarnykh raznovidnostei al'dolazy cheloveka i krysy s pomoshch'iu élektroforeza na krakhmal'nom i agarovom geliakh.

Content of aldolase isoenzymes was reinvestigated in human and rat tissues by means of agar and starch gel electrophoresis. Heterogeneity of the A type aldolase is established and possible causes of formation of the multiple forms of the enzyme are discussed. The ratio of various fractions of aldolase A was different in erythrocytes of newborn and aldult persons. The patterns of the aldolase isozyme spectra were characterized in blood sera of newborns, in acute hepatitis and myocardial infarction.

[Intracellular distribution of creatine kinase isoenzymes in the brains and hearts of rats at different stages of postnatal development]. / Vnutrikletochnoe raspredelenie izofermentov kreatinkinazy v golovnom mozge i serdtse krys na razlichnykh stadiiakh postnatal'nogo razvitiia

The total activity and range of the creatine kinase (CK) isozymes have been studied in the homogenate and subcellular fractions (nuclei, mitochondria, cytoplasm) of the rat brain and heart during postnatal ontogenesis. The total activity of CK in the brain and heart of newborn rats was found to be 4 and 2 times less, resp., than in those of adults. The age patterns were established in the activity of cytoplasmic (CK-1, CK-2 and CK-3) and mitochondrial (CK-4) isozymes. During the whole postnatal development the rat brain contains only one cytoplasmic isozyme, CK-1. In the heart of newborn rats, as compared with adults, the content of CK-1 and CK-2 is much higher and that of CK-3 lower. On the 12-15th day of life the range of the CK isozymes approaches that characteristic of adult animals. The activity of CK-4 was found in the brain on the 5-7th day of life and in the heart on 12-15th day. In the range of the CK isozymes in the adult brain the content of mitochondrial CK amounts to 19.3% and in the heart to 16.5%. The data obtained complement the literary ones suggesting the low level of energy-forming processes in the brain and heart cells at the early stages of the rat postnatal development.