Scientific risk performance analysis and development of disaster management framework: A case study of developing Asian countries.

Disaster is a state of serious disruptions in the functionality of any society or county. Disasters pose serious economic or environmental impacts that surpass the capacity of the affected country or society to compete with the use of their assets. Recently, Pakistan significantly prone to health disasters due to COVID-19 among developing South Asian countries. The long-term impact of health disasters and other natural hazards put additional pressure mostly on the government's economic policy. It forces the government to follow a constructive approach like a disaster relief-based approach rather than a conventional mitigation management formation to reduce the impact of disaster risk. This study elaborates on the main issues associated with disaster preparedness as well as recovery of the economy and businesses of the country. For Scientific risk performance analysis, open-source data from the National Institute of Disaster Management (NDMA) has been utilized to study the current situation of COVID-19 in Pakistan. Results show Pakistan has been facing a highly vulnerable situation as more than three hundred and fifty thousand confirmed cases have been reported. Poor health and technical management facilities have been exposed against COVID-19 as Pakistan has a low heath budget because of its declining GDP growth rate in the world. This research will help in disaster preparedness and the development of a disaster risk management framework while designing strategies to deal with such pandemics in the future.

Investigation of Cytotoxicity Apoptotic and Inflammatory Responses of Biosynthesized Zinc Oxide Nanoparticles from Ocimum sanctum Linn in Human Skin Keratinocyte (Hacat) and Human Lung Epithelial (A549) Cells.

Pristine and engineered metal nanoparticles are widely applied in various fields of industry, and as consequences, they are useful as well as harmful to human health and environment. This study aimed at synthesizing the green zinc oxide nanoparticles (ZnNPs) using the leaf extract of Ocimum sanctum Linn and assessing its toxicity on human skin epidermal (HaCaT) and human lung epithelial (A549) cells. The synthesized green ZnNPs (gZnNPs) were characterized by using dynamic light scattering (DLS) and a high-resolution transmission electron microscope. The average size of gZnNPs obtained was 62 nm with a spherical shape. The effects of gZnNPs on the viability of HaCaT and A549 cells were investigated using tetrazolium salt (MTT) for 24 h. We have seen more reduction of cell viability of A549 cells in comparison to HaCaT cells. The induction of intracellular reactive oxygen species (ROS) was measured using DCFDA assay and showed a slightly high intensity of green fluorescence in A549 than HaCaT cells. The different oxidative stress biomarkers such as ROS generation and lipid peroxide were increased, and GSH was decreased in a dose-dependent manner. The apoptotic and necrotic effect of gZnNPs in both cells was carried out using Annexin-V-FITC and propidium iodide staining. More apoptotic and necrotic cells were found at a higher concentration of gZnNPs exposure. Also, we determined the effect of gZnNPs at the molecular level by evaluating the apoptotic and inflammatory markers, in which gZnNPs downregulated Bcl2 and upregulated Bax, caspase-3, and TNF-α in HaCaT and A549 cells. Ultimately, gZnNPs exerted toxicity and apoptosis in HaCaT and A549 cells.