Assessing the influence of sewage outfalls on seagrass meadows using nitrogen isotopes.

Untreated sewage discharged increases the nutrient loads and changes ecosystem functions. It increases the values of the nitrogen isotopic signature (δ15N) of primary producers such as seagrasses. Itaparica Island (Bahia, Brazil) has undergone extensive urbanization over 50 years. Most of the island has no sewage treatment, and a bridge's construction could increase its population ten times. We evaluated the effects of sewage inputs on the δ15N of seagrass (Halodule wrightii) across Itaparica Island in 14 areas of the island with different degrees of urbanization. Average values of δ15N ranged from -3.95 ‰ (±1.04 SD) to 2.73 ‰ (±1.61). The highest human occupation site also has the highest mean value of δ15N, and seagrass shoot density. The significant correlation (p < 0.05) between δ15N values and shoot density may indicate a possible anthropogenic pressure impacting meadow abundance. Despite a positive correlation, increased anthropogenic nutrient supply can support algae growth and harm seagrass ecosystems.

Evidence of sewage input to inner shelf sediments in the NE coast of Brazil obtained by molecular markers distribution.

Coprostanol (5ß-cholestan-3ß-ol) and other fecal steroids were used as markers to trace the input of sewage to the shelf along the coast of Sergipe and Alagoas States, in NE Brazil. Surface sediment samples in shallow regions (<50 m depth) on the shelf were collected adjacent to four fluvial systems, whose drainage basins exhibit distinct levels of human influence. Highest coprostanol concentrations were observed in the area under influence of the Sergipe River (58.6 ± 74.6 ng g(-)(1); maximum of 184.1 ng g(-)(1)), whereas in the Vaza-Barris (18.9 ± 23.6 ng g(-)(1)) and the Piaui/Real (12.3 ± 6.7 ng g(-)(1)) rivers the levels of coprostanol were near the method's limit of detection. Nearby the São Francisco River, the largest in the NE Brazil, coprostanol was virtually absent. The findings of the present study, supported by coprostanol-based diagnostic ratios, revealed that, when sanitation is lacking in the coastal region, even a small-sized river like the Sergipe River can effectively contribute to the export of sewage-derived organic matter to the inner continental shelf in the studied region.