RESUMO
The current study aimed to prepare a topical gel containing solid lipid nanoparticles (SLNs) encapsulating fluoxetine for diabetic wound healing effects. Fluoxetine (FX) was loaded into SLNs by employing an emulsion solvent evaporation technique using stearic acid as a lipid, and soya lecithin as a surfactant. SLNs were then evaluated for particle size, polydispersity index (PDI), zeta potential (ZP), percent entrapment efficiency (%EE), percent drug loading (%DL), and in vitro drug release. The optimized SLN (FS3) composed of FX (100 mg), SA (150 mg), and SA (100 mg) displayed mean particle size (467.3 ± 2.2nm), PDI (0.435 ± 0.02), ZP (-32.2 ± 4.47mV), EE (95.8 ± 3.38%), and DL (16.4 ± 2.4%). FTIR and DSC studies denote drug-polymer compatibility and the amorphous nature of FX in the SLNs. The drug release at 24 h was found to be (98.89 ± 2.57%) which followed the fickian diffusion mechanism. SLN (FS3) was further loaded into carbopol gel and tested for pH, spreadability, and extrudability of pharmaceutical parameters. In-vitro release of FX from the SLN gel and plain gel was compared, diabetic wound healing gel (DWH) showed sustained drug delivery. An in vivo study was also performed for DWH gel in streptozotocin-induced diabetic rats. Histopathological examination exhibited DWH gel-treated wounds have increased hydroxyproline, cellular proliferation, a rise in the number of blood vessels, and the level of collagen synthesis. Thus, DWH gel-loaded SLN encapsulated with FX could be a potential carrier for the effective treatment and management of diabetic wounds.
RESUMO
AIM: To assess and analyze the resin-bonded dentin in terms of shear bond strength after using deproteinizing agents 5% sodium hypochlorite, 10% sodium hypochlorite, and bromelain. MATERIALS AND METHODS: Overall 140 permanent and intact human premolars were split into four groups, three experimental groups and one control group. In all four groups, the occlusal surface of the teeth was wet ground to expose superficial dentin. In group I, teeth were etched and deproteinized with 5% sodium hypochlorite. In group II, teeth were etched and deproteinized with 10% sodium hypochlorite. In group III, teeth were etched and bromelain was used to deproteinize. In group IV, teeth were etched and no deproteinization was being performed and simultaneous fulfillment of the resin composite and later inserted into the plastic tube and polymerized with light. Samples were stored at 37°C for 24 hours and the later samples were transferred to the universal testing machines to shear bond strength analysis at a speed of 0.5 mm/minute. RESULTS: The outcome of the bond strength was significantly influenced by the application of bromelain enzymes. A remarkable difference was observed between the shear bond strength of sodium hypochlorite (5 and 10%) and in the bromelain enzyme-treated group. Group III showed better results than group I and group II. CONCLUSION: This study concluded that bromelain shear has the maximum value for shear bond strength. Bond strength improved because of removal of unsupported collagen fiber with bromelain enzyme after acid etching. CLINICAL SIGNIFICANCE: Natural pineapple enzyme, i.e., bromelain improves bond strength by removal of unsupported collagen fiber. Hence, it is completely safe to use. HOW TO CITE THIS ARTICLE: Khan R, Sharma N, Garg Y, et al. Comparison of Different Dentin Deproteinizing Agents on the Shear Bond Strength of Resin-bonded Dentin. Int J Clin Pediatr Dent 2020;13(S-1):S69-S77.
