RESUMO
Chagas disease (CD) is a neglected disease caused by Trypanosoma cruzi Chagas, 1909. Causative treatment can be achieved with two drugs: benznidazole or Nifurtimox. There are some gaps that hinder progress in eradicating the disease. There is no test that can efficiently assess cure control after treatment. Currently, the decline in anti-T. cruzi antibody titres is assessed with conventional serological tests, which can take years. However, the search for new markers of cure must continue to fill this gap. The present study aimed to evaluate the decline in serological titres using chimeric proteins after treatment with benznidazole in chronic patients diagnosed with CD. It was a prospective cross-sectional cohort study between 2000 and 2004 of T. cruzi-positive participants from the Añatuya region (Argentina) treated with benznidazole. Serum samples from ten patients were collected before treatment (day zero) and after the end of treatment (2, 3, 6, 12, 24 and 36 months). For the detection of anti-T. cruzi antibodies, an indirect ELISA was performed using two chimeric recombinant proteins (IBMP-8.1 and IBMP-8.4) as antigens. The changes in reactivity index within the groups before and after treatment were evaluated using the Friedman test. All participants experienced a decrease in serological titres after treatment with benznidazole, especially IBMP-8.1. However, due to the small number of samples and the short follow-up period, it is premature to conclude that this molecule serves as a criterion for sustained cure. Further studies are needed to validate tests based on these or other biomarkers to demonstrate parasitological cure.
Assuntos
Doença de Chagas , Nitroimidazóis , Trypanosoma cruzi , Humanos , Estudos Transversais , Estudos Prospectivos , Doença de Chagas/tratamento farmacológico , Proteínas Recombinantes de Fusão/uso terapêuticoRESUMO
The nutrient uptake of cotton suspension cultures was studied during a 30 day-culture period. Cotton cell suspension cultures of the 101-102B genotype were established and monthly subcultured in liquid medium. Culture growth rate, nutrient uptake and pH variations in the medium were measured. A 6-fold increase in fresh weight of the cotton cell culture was observed on day 30. The majority of the nutrients were taken up at a high rate, and their consumption was at least 80% of the original concentration. Nevertheless, the uptake rate varied among the elements. While PO4(3-) was practically depleted from the medium on day 10, the ions such as K+, Ca2+, SO4(2-) and Mg2+ had a 20% residual at the end of the period. The pH of the culture medium increased initially and stabilized at pH 6.2 at the beginning of the stationary phase. In addition, the cotton cell suspension culture took up 89% of glucose added to the medium.
Com o objetivo de conhecer os requerimentos nutricionais de suspensões celulares de algodoeiro foi feita uma análise da utilização de nutrientes no intervalo de 30 dias, normalmente utilizado entre subculturas. As culturas de células em suspensão de algodoeiro, genótipo 101-102B, foram estabelecidas e subcultivadas mensalmente em meio líquido. Foram analisadas o crescimento das culturas, as variações de pH do meio de cultura e a absorção de nutrientes. A matéria fresca das culturas de células foi superior a 6 vezes o valor inicial após 30 dias de cultivo. Houve aumento do pH no início da cultura, estabilizando-se em 6,2 próximo à fase estacionária. Durante o crescimento das suspensões, as células consumiram pelo menos 80% da concentração inicial da maioria dos nutrientes quantificados. A velocidade de absorção, entretanto, variou entre os elementos. Enquanto PO4(3-) foi praticamente esgotado do meio após 10 dias de cultivo, os íons K+, Ca2+, SO4(2-) e Mg2+ foram encontrados em concentrações próximas a 20% do valor inicial após 30 dias. As suspensões de células de algodoeiro consumiram 89% da concentração inicial de glicose.
RESUMO
The nutrient uptake of cotton suspension cultures was studied during a 30 day-culture period. Cotton cell suspension cultures of the 101-102B genotype were established and monthly subcultured in liquid medium. Culture growth rate, nutrient uptake and pH variations in the medium were measured. A 6-fold increase in fresh weight of the cotton cell culture was observed on day 30. The majority of the nutrients were taken up at a high rate, and their consumption was at least 80% of the original concentration. Nevertheless, the uptake rate varied among the elements. While PO4(3-) was practically depleted from the medium on day 10, the ions such as K+, Ca2+, SO4(2-) and Mg2+ had a 20% residual at the end of the period. The pH of the culture medium increased initially and stabilized at pH 6.2 at the beginning of the stationary phase. In addition, the cotton cell suspension culture took up 89% of glucose added to the medium.
Com o objetivo de conhecer os requerimentos nutricionais de suspensões celulares de algodoeiro foi feita uma análise da utilização de nutrientes no intervalo de 30 dias, normalmente utilizado entre subculturas. As culturas de células em suspensão de algodoeiro, genótipo 101-102B, foram estabelecidas e subcultivadas mensalmente em meio líquido. Foram analisadas o crescimento das culturas, as variações de pH do meio de cultura e a absorção de nutrientes. A matéria fresca das culturas de células foi superior a 6 vezes o valor inicial após 30 dias de cultivo. Houve aumento do pH no início da cultura, estabilizando-se em 6,2 próximo à fase estacionária. Durante o crescimento das suspensões, as células consumiram pelo menos 80% da concentração inicial da maioria dos nutrientes quantificados. A velocidade de absorção, entretanto, variou entre os elementos. Enquanto PO4(3-) foi praticamente esgotado do meio após 10 dias de cultivo, os íons K+, Ca2+, SO4(2-) e Mg2+ foram encontrados em concentrações próximas a 20% do valor inicial após 30 dias. As suspensões de células de algodoeiro consumiram 89% da concentração inicial de glicose.