RESUMO
AECR mutants of Bacillus subtilis were obtained and analyzed. The mutants were characterized by derepression of aspartokinase II and diaminopimelate decarboxylase synthesis and the synthesis of the precursor of lysine--DAP. According to genetic mapping data, aec mutations are localized in some B. subtilis chromosomal regions; they are linked to the thr5, leuA8, lys21 markers.
Assuntos
Bacillus subtilis/genética , Proteínas de Bactérias , Cisteína/análogos & derivados , Mutação , Aspartato Quinase/antagonistas & inibidores , Aspartato Quinase/metabolismo , Bacillus subtilis/efeitos dos fármacos , Bacillus subtilis/metabolismo , Carboxiliases/antagonistas & inibidores , Carboxiliases/metabolismo , Mapeamento Cromossômico , Cisteína/farmacologia , Ácido Diaminopimélico/metabolismo , Resistência Microbiana a Medicamentos , Ligação GenéticaRESUMO
A number of B. subtilis mutants auxotrophic for lysine has been isolated and mapped in relation to the flanking ser2 marker. One of the mutants has been found to have a mutation in lys A gene coding for DAP-decarboxylase activity. The expression of DAP-decarboxylase is dependent on the product of lys R gene that is not linked with lysine genes cluster.
Assuntos
Bacillus subtilis/genética , Lisina/genética , MutaçãoRESUMO
Hybrid plasmids pLRS33 and pLRB4 containing Bac. subtilis genes coding lysin biosynthesis were subjected to genetical analysis. It is shown that after pLRS33- and pLRB4- transformation of E. coli strains, auxotrophic relative to lysin and diaminopimelic acid, there occurs complementation of dapA, dapB, dapC, dapD, dapE, lysA mutations by plasmid pLRS33 and of dapC, dapB, lysA mutations by plasmid pLRB4. The plasmids are studied for their influence on the level of lysin and its precurror synthesis in E. coli strains.