Evaluation of a custom Sensititre YeastOne plate for susceptibility testing of isavuconazole and other antifungals against clinically relevant yeast and mould species in three Australian diagnostic mycology laboratories.

This study aimed to validate the performance of the custom formulated Sensititre YeastOne One (SYO) microdilution plate which includes isavuconazole (AUSNMRC1) to perform susceptibility testing on clinically relevant yeast and mould species across three Australian reference laboratories. The minimum inhibitory concentration (MIC) results were compared with the IVD approved SYO YO10 microdilution plate and isavuconazole gradient strips. A total of 127 isolates were tested on both the YO10 and AUSNMRC1 plates. The overall essential agreement (EA) and categorical agreement (CA) for the eight common drugs was 99.9% and 98.8%, respectively. The EA was 96.9% for the isavuconazole MICs obtained using the AUSNMRC1 plate and gradient strip. The MIC results for all nine antifungals on the AUSNMRC1 panel were highly reproducible for all quality control and reference strains and the overall EA and CA for 45 clinical strains tested across all three participating laboratories were >93% and 94.1%, respectively. These findings demonstrate the SYO AUSNMRC1 plate provides a commercial means to determine isavuconazole MICs by broth microdilution testing.

A comparison of methods used for quantifying internal training load in women soccer players.

PURPOSE: The purpose of this study was to compare the session-RPE method for quantifying internal training load (TL) with various HR-based TL quantification methods in a variety of training modes with women soccer players. METHODS: Fifteen elite women soccer players took part in the study (age: 19.3 +/- 2.0 y and VO2max: 50.8 +/- 2.7 mL x kg(-1) x min(-1)). Session-RPE, heart rate, and duration were recorded for 735 individual training sessions and matches over a period of 16 wk. Correlation analysis was used to compare session-RPE TLs with three commonly used HR-based methods for assessing TL. RESULTS: The mean correlation for session-RPE TL with Banister's TRIMP, LTzone TL and Edwards's TL were (r = 0.84, 0.83, and 0.85, all P < .01, respectively). Correlations for session-RPE TL and three HR-based methods separated by session type were all significant (all P < .05). The strongest correlations were reported for technical (r = 0.68 to 0.82), conditioning (r = 0.60 to 0.79), and speed sessions (r = 0.61 to 0.79). CONCLUSION: The session-RPE TL showed a significant correlation with all training types common to soccer. Higher correlations were found with less intermittent, aerobic-based training sessions and suggest that HR-based TLs relate better to session-RPE TLs in less intermittent training activities. These results support previous findings showing that the session-RPE TL compares favorably with HR-based methods for quantifying internal TL in a variety of soccer training activities.