Knowledge attitude and practices of university students to Vitamin D and Vitamin D supplements during times of low sun exposure and post lockdown.

OBJECTIVE: Vitamin D deficiency is prevalent in Saudi Arabia. Inability to get sun exposure greatly influences Vitamin D levels. Getting enough Vitamin D during childhood and young adulthood can prevent possible future diseases. We aim to estimate the level of knowledge of Saudi University students about Vitamin D and Vitamin D supplements, to correlate it with their attitudes and practices to sun exposure and Vitamin D supplements and to compare between males and females. MATERIALS AND METHODS: This is an epidemiological cross-sectional KAP study conducted at King Saud University in Riyadh. A stratified random sampling technique was used where students were randomly selected from 3 colleges and stratified to males and females. N= 767 students completed the online survey used to assess the knowledge, attitudes, and practices of the students. RESULTS: We found that the level of knowledge of university students was moderate to low (mean equivalent to 40%) whereas females had better knowledge. Also, their practices are not sufficient to maintain healthy Vitamin D levels as only 8.2% stay in the sun for more than an hour during weekdays. Only 10.2% of students take regular Vitamin D supplements, where females are the more likely users. 99.1% of students stay indoors during work. CONCLUSIONS: The knowledge about Vitamin D is low among university students but slightly higher in females and medical students. Almost all participants work indoors and most of them wear a mask whenever going out. Few students take regular Vitamin D supplements. Females agreed more they have Vitamin D deficiency and take supplements more than males.

Silencing of tissue factor by antisense deoxyoligonucleotide mitigates thioacetamide-induced liver injury.

BACKGROUND: Retinoid receptors (RRs), RAR-α and RXR-α, work as transcription factors that regulate cell growth, differentiation, survival, and death. Hepatic stellate cells (HSCs) store retinoid and release its RRs as lipid droplets upon their activation. PURPOSE: We test the hypothesis that loss of retinoid receptors RAR-α and RXR-α from HSCs is dependent on tissue factor (TF) during thioacetamide (TAA)-induced liver injury. METHODS: Liver toxicity markers, TF, fibrin, cleaved caspase-3, and cyclin D1 as well as histopathology were investigated. RESULTS: Increased TF, fibrin, cleaved caspase-3, and cyclin D1 protein expression is seen in zone of central vein after TAA injection compared with vehicle-treated mice. A strong downregulation of RAR-α and RXR-α is seen in TAA-induced liver injury. In addition, histopathological obliteration and pericentral expression of cleaved caspase 3 and cyclin D1 are observed after TAA injection compared with the normal vehicle-treated mice. No changes have been seen in TAA/TF-sense (SC) in whole parameters compared with TAA-treated animals. TAA/TF-antisense (AS)-treated mice show normal expression of all parameters and normal histopathological features when compared with the control mice. In conclusion, this study declares that the strong downregulation of RAR-α and RXR-α may cause liver injury and particularly activation of HSCs in TAA-induced toxicity. TF-AS treatment not only downregulates TF protein expression but also alleviates loss of liver RAR-α and RXR-α and suppresses the activated apoptosis signals in TAA-induced liver toxicity. Finally, TF and RAR-α/RXR-α are important regulatory molecules in TAA induced acute liver injury.