Increased oxidative stress and altered serum macro-minerals and trace elements levels are associated with coronary artery disease.

BACKGROUND: This study was designed to evaluate the serum malondialdehyde (MDA), non-enzymatic antioxidants (vitamin A and C), macro-minerals (magnesium and calcium), and trace elements (zinc, copper, and iron) levels in patients with coronary artery disease (CAD) and to explore their role in disease progression. METHODS: This prospective case-control study was comprised of 40 CAD patients and 40 healthy volunteers as cases and control subjects, respectively. The level of lipid peroxidation was assessed by measuring the serum MDA level using a UV spectrophotometer. The levels of vitamins A and C were determined by high-performance liquid chromatography (HPLC) and UV spectrophotometric method, respectively. Atomic absorption spectroscopy (AAS) was used to measure serum macro-minerals (Mg and Ca) and trace elements (Zn, Cu, and Fe) concentrations. RESULTS: The mean age of CAD patients and control subjects was 53.90 ± 2.22 and 37.03 ± 1.50 years, respectively. This study revealed significantly higher concentrations of MDA (p < 0.01) and lower concentrations of vitamin A (p < 0.01), and vitamin C (p < 0.05) in the CAD patients than in control subjects. The mean values of Mg, Cu, Zn, Ca, and Fe were 11.67 ± 0.64, 1.17 ± 0.03, 0.43 ± 0.02, 107.38 ± 1.81, and 1.66 ± 0.04 µg/mL, respectively for the CAD patients and 19.38 ± 0.65, 1.07 ± 0.02, 0.87 ± 0.02, 94.29 ± 1.89, and 1.52 ± 0.05 µg/mL, respectively for the controls and the differences were significant (p < 0.05) between the patients and controls. CONCLUSION: From these findings, we can suggest that there is a strong association of CAD with an elevated level of MDA, depleted levels of antioxidants, and altered macro-minerals and trace elements concentrations.

Antiviral activity of the inducible humoral immunity and its suppression by eleven BEN family members encoded in Cotesia plutellae bracovirus.

Upon parasitization by some endoparasitoids, polydnaviruses (PDVs) play a crucial role in inducing host immunosuppression. This study reports a novel immunosuppressive activity against humoral immune responses by BEN family genes encoded in Cotesia plutellae bracovirus (CpBV). A total of 11 BEN family members are encoded in 10 different CpBV DNA segments. When the CpBV segments were individually injected, specific BEN genes were expressed and suppressed the expression of antimicrobial peptide (AMP) and prophenoloxidase genes following bacterial challenge. The suppressive activities of the BEN genes were reversed by injection of the double-stranded RNA (dsRNA) specific to each BEN gene. The suppression of the AMP gene expressions by the BEN genes was also confirmed using an inhibition zone assay against Gram-positive and Gram-negative bacterial growth. The significance of the suppressive activity of BEN genes against humoral immune responses was analyzed in terms of suppression of antiviral activity by the host humoral immunity. When CpBV was incubated with the plasma obtained from the larvae challenged with bacteria, the immunized plasma severely impaired the expression activity of the viral genes. However, an expression of BEN gene significantly rescued the viral gene expression by suppressing humoral immune response. These results suggest that BEN family genes of CpBV play a crucial role in defending the antiviral response of the parasitized Plutella xylostella by inhibiting humoral immune responses.

Phytochemical and in vitro biological investigations of methanolic extracts of Enhydra fluctuans Lour.

OBJECTIVE: To study the phytochemical and biological properties (antioxidant, anthelmintic and thrombolytic) of methanolic extracts of Enhydra fluctuans Lour., a plant belonging to the Asteraceae family. METHODS: The phytochemical evaluation was carried out by qualitative analysis. In vitro antioxidant activity of extract was studied using free radical scavenging assay, ability of reduction, total phenol and total flavonoid contents determination assays. The anthelmintic activity was determined using paralysis and death time of Pheretima posthuma (earthworm) and thrombolytic activity by clot disruption assay. RESULTS: The phytochemical evaluation showed significant presence of flavonoids, triterpenes, carbohydrate, reducing sugars, saponins, phenols, diterpenes, protein and tannin. The antioxidant activity was found significant [IC50=(135.20±0.56) µg/mL] as compared to ascorbic acid [(130.00±0.76) µg/mL]. The reducing power was increased with concentration. Total phenol and total flavonoid contents were (153.08±0.38) mg/mL and (172.04±0.56) mg/mL respectively. The paralysis and death time of earthworms for different concentrations of extract were determined and compared with albendazole. The results showed that 10 mg/mL of the crude extract had similar effect with albendazole. Additionally, the crude extract showed a concentration depended relationship with its anthelmintic property. The clot lysis activity of crude extract was compared to the standard streptokinase's clot lysis (40.13%) activity and found significant (31%). CONCLUSIONS: The study proves that the crude methanolic extract of Enhydra fluctuans Lour. has significant antioxidant, anthelmintic and thrombolytic activity containing wide range of phytochemicals.

Aspect of thrombolytic therapy: a review.

Thrombolytic therapy, also known as clot busting drug, is a breakthrough treatment which has saved untold lives. It has been used in the clinical area to treat venous and arterial thromboembolic complaints which are a foremost cause of death. In 1761, Morgagni lead the way of thrombolytic therapy. Now day's different types of thrombolytic drugs are currently available in market: alteplase, anistreplase, urokinase, streptokinase, tenecteplase, and so forth. Thrombolytic therapy should be given with maintaining proper care in order to minimize the risk of clinically important bleeding as well as enhance the chances of successfully thrombolysis of clot. These cares include preinfusion care, during the infusion care, and postinfusion care. Besides proper knowledge of contraindication, evolutionary factor, and combination of drug is essential for successful thrombolytic therapy. In these review we discussed about these aspect of thrombolytic therapy.

Investigation of Phenolic Profiles, Cytotoxic Potential and Phytochemical Screening of Different Extracts of Drynaria quercifolia J. Smith (Leaves).

PURPOSE: The present study is aimed to evaluate phenolic profiles, cytotoxic activity and phytochemical screening of different extracts of Drynaria quercifolia leaves. METHODS: The dried and powder leaves were extracted with methanol at room temperature and the concentrated methanolic extract was fractionated by the modified Kupchan partitioning method to provide pet-ether, carbon tetrachloride, chloroform and aqueous soluble fractions. Phenolic profiles were determined by using Folin-Ciocalteau reagent, which results were expressed in gallic acid equivalent (mg of GAE/g of sample). Phytochemical properties of different extractives of plant materials were tested by the method of Trease and Evans. Brine shrimp lethality bioassay was used to investigate the cytotoxic potential of D. quercifolia. RESULTS: The phytochemical screening revealed the potent source of different phytochemical constituents on different extractives including alkaloid, glycosides, tannin, saponins, proteins and amino acids, flavonoids, triterpenes, phenols, phytosterols and carbohydrate. In the determination of phenolic profiles, different extractives showed a significant content of phenolic compounds ranging from 103.43 -132.23 mg of GAE/g of extractive. Compared to vincristine sulfate different extractives of plant materials demonstrated moderate cytotoxic potential (having LC50 of 12.45 µg/ml, 13.02 µg/ml 15.83 µg/ml, 14.95 µg/ml and 7.612 µg/ml, respectively). CONCLUSION: It is concluded from this study that D. quercifolia is an excellent source of phenolic content and phytoconstitutes as well as possesses moderate cytotoxic activity.

Teratocyte-secreting proteins of an endoparasitoid wasp, Cotesia plutellae, prevent host metamorphosis by altering endocrine signals.

An endoparasitoid wasp, Cotesia plutellae, parasitizes young larvae of the diamondback moth, Plutella xylostella, with its parasitic factors of polydnavirus, venom, ovarian proteins, and teratocytes (TCs). TCs are originated from embryonic serosal membrane at hatch of C. plutellae egg. Injection of in vitro cultured TCs significantly prolonged a larval period of nonparasitized P. xylostella and impaired a larva-to-pupa metamorphosis. This developmental alteration was also induced by injection of TC-cultured medium (TCM). However, heat-treated TCM significantly lost the inhibitory activity against larval development of P. xylostella. Larvae treated with TC or TCM appeared to undergo abnormal endocrine conditions. Juvenile hormone esterase activity was significantly suppressed at early last instar by injection of TC or TCM. In addition, expression of ecdysone receptor at final instar was lost, but that of insulin receptor was maintained until the end of the larval period in TC or TCM treatment. A proteomic analysis of TCM predicted several teratocyte-secreting proteins (TSPs). The inhibitory effect of host development by TCs was significantly enhanced by an addition of another parasitic factor, C. plutellae bracovirus. These results suggest that C. plutellae TC plays a crucial role in alteration of host development by secreting TSPs.