Diet, Plasma, Erythrocytes, and Spermatozoa Fatty Acid Composition Changes in Young Vegan Men.

There has been increasing interest in vegan diets, but how this dietary pattern regulates tissue fatty acids (FA), especially in men, is unclear. Our aim was to evaluate the effect of a vegan diet on plasma, erythrocyte, and spermatozoa FA composition in young men. Two groups consisting of 67 young (18-25 years old) men were studied. One group following an omnivore diet but did not consume fish, shellfish or other marine foods (control, n = 33), and another group following a vegan diet (vegan, n = 34) for at least 12 months were compared. Dietary intake was assessed via a food frequency questionnaire and a 24-h recall. FA composition was measured in plasma, erythrocyte phospholipids, and spermatozoa by gas-liquid chromatography. Compared to controls, the vegan group had higher reported intakes of carbohydrate, dietary fiber, vitamins (C, E, K, and folate), and minerals (copper, potassium) but lower intakes of cholesterol, trans FA, vitamins B6 , D, and B12 , and minerals (calcium, iron, and zinc). Vegan's reported a lower saturated FA and not arachidonic acid intake, both groups did not intake eicosapentaenoic acid and docosahexaenoic acid (DHA), but vegan's showed a higher alpha linolenic acid ALA intake. Vegans had higher plasma, erythrocyte phospholipid, and spermatozoa ALA, but lower levels of other n-3 polyunsaturated fatty acid (PUFA), especially DHA. Vegans were characterized by higher ALA, but lower levels of other n-3 PUFA, especially DHA in plasma, erythrocytes, and spermatozoids. The biological significance of these findings requires further study.

Aislamiento e identificación de Vibrio parahaemolyticus O3: K6 en pescados y moluscos bivalvos procedentes de un mercado pesquero de Lima, Perú / Isolation and identification of Vibrio parahaemolyticus O3: K6 in fish and bivalve mollusks of a fish market

Objetivo: Determinar la presencia de Vibrio parahaemolyticus serotipo O3:K6 en pescados y moluscos bivalvos procedentes de un mercado pesquero de Lima. Material y Métodos: Se analizaron 254 muestras entre pescados y moluscos bivalvos siguiendo la técnica descrita por Yukiko Harakudo et al, que consta de un paso de enriquecimiento, siembra en medio cromogénico, evaluación de fermentación de glucosa y la prueba de tolerancia a sales. Las cepas identificadas como Vibrio parahaemolyticus fueron serotipificadas usando anticuerpos monoclonales y evaluadas en su potencial patogénico en medio de Wagatsuma. Resultados: Se aislaron 15 cepas de V. parahaemolyticus, 9(7,5%) en pescados y 6(4,5%) en moluscos bivalvos. Tres (20%) presentaron fenómeno de Kanagawa positivo indicando la producción de la hemolisina termoestable directa (TDH). Una cepa TDH-positiva aislada en una muestra pescado fue serotipo O3:K6. Conclusiones: Vibrio parahaemolyticus se encuentra distribuido en los recursos hidrobiológicos estudiados con mayor frecuencia en pescados, a partir de los cuales se encontró el serovar O3:K6. Este es el primer reporte de la cepa pandémica O3:K6 de Vibrio parahaemolyticus aislada de fuente no humana en el Perú.

Objective: To determine the presence of Vibrio parahaemolyticus serotype O3:K6 in fish and bivalves mollusks of a fish market from Lima. Material and methods: We analyzed 254 samples of fish and bivalve mollusks using the technique described by Yukiko Harakudo et al, consisting of an enrichment step, chromogenic plating, evaluation of glucose fermentation and salt tolerance test. The strains identified as Vibrio parahaemolyticus were serotyped using monoclonal antibodies and evaluated their pathogenic potential on Wagatsuma agar. Results: 15 strains of V. parahaemolyticus, 9 (7,5%) in fish and 6 (4,5%) in bivalve mollusks were isolated. 3 (20%) were Kanagawa phenomenon positive indicating the production of thermostable direct hemolysin (TDH). A TDH-positive strain isolated from a fish belonged to the serotype O3: K6. Conclusions: Vibrio parahaemolyticus is distributed in analyzed aquatic resources; most frequently in fish from which was found serovar O3:K6. This is the first report of pandemic O3:K6 strain of Vibrio parahaemolyticus isolated from non-human source in Perú.

Más allá de la intimidad: cinco estudios en sexualidad, salud sexual y reproductiva / Beyond intimacy: five studies on sexuality, sexual and reproductive health

Contiene: Ente patas y paltas: parejas sexuales, riesgos sexuales y redes personales entre jóvenes varones de Barrios Altos; Placer, riesgo y poder: corresponsabilidad y negociación de hombres y mujeres respecto al uso de métodos anticonceptivos; Percepciones y comportamientos frente a la usuaria adolescente; El ciclo de violencia doméstica y las estrategias de afrontamiento de un grupo de mujeres denunciantes; Daño moral derivado del divorcio: es percibido por las partes que intervienen en el proceso legal?