Assuntos
Farmacorresistência Bacteriana/genética , Macrolídeos/uso terapêutico , Infecções Estafilocócicas/microbiologia , Staphylococcus/genética , Antibacterianos/uso terapêutico , Proteínas de Bactérias/genética , Genótipo , Humanos , Irã (Geográfico) , Proteínas de Membrana/genética , Proteínas de Ligação às Penicilinas , Fenótipo , Infecções Estafilocócicas/tratamento farmacológico , Staphylococcus/isolamento & purificaçãoRESUMO
The purpose of this study was to determine the time required for anaerobic bacteria in natural human saliva to contaminate root-filled teeth. Thirty-two single-rooted teeth were cleaned, shaped, filled, and exposed to human saliva for 120 days. Teeth that had not leaked were subjected to polymerise chain reaction examination. Sixty-six percent of the experimental group were totally contaminated. A PCR examination revealed there was no contamination in the apical 3 mm of leakage-free teeth.
Assuntos
Bactérias Anaeróbias/isolamento & purificação , Infiltração Dentária/microbiologia , Cavidade Pulpar/microbiologia , Obturação do Canal Radicular/métodos , Saliva/microbiologia , Bactérias Anaeróbias/classificação , Bismuto/uso terapêutico , DNA Bacteriano/análise , Infiltração Dentária/classificação , Combinação de Medicamentos , Eletroforese em Gel de Ágar , Resinas Epóxi/uso terapêutico , Guta-Percha/uso terapêutico , Humanos , Reação em Cadeia da Polimerase , Materiais Restauradores do Canal Radicular/uso terapêutico , Irrigantes do Canal Radicular/uso terapêutico , Obturação do Canal Radicular/instrumentação , Preparo de Canal Radicular/instrumentação , Preparo de Canal Radicular/métodos , Prata/uso terapêutico , Hipoclorito de Sódio/uso terapêutico , Temperatura , Fatores de Tempo , Titânio/uso terapêutico , Ápice Dentário/microbiologiaRESUMO
Multiple drug-resistant strains of Acinetobacter have created therapeutic problems worldwide. This study was conducted to determine the antimicrobial susceptibility patterns and prevalence of bla(OXA-type) carbapenemases among isolates of Acinetobacter spp. obtained from Iranian patients. Here, 128 Acinetobacter isolates were identified at the species level, and their susceptibilities to different antibiotics were determined using disk agar diffusion testing. Isolates were then subjected to multiplex-PCR targeting bla(OXA) genes. More than 50% of the isolates showed multidrug resistance to different antibiotics. The rates of susceptibility to imipenem, meropenem, piperacillin-tazobactam, and amikacin were 50.7, 50, 42.1, and 38.2%, respectively. The MICs of carbapenems for the resistant isolates ranged from 64 to > or = 256 microg/ml. All strains of Acinetobacter baumannii possessed a bla(OXA-51-like) gene. The co-existence of bla(OXA-51-like)/bla(OXA-23-like) and bla(OXA-51-like)/bla(OXA-24-like) was detected in 25% (n=32) and 17.9% (n=23) of the isolates, respectively. Over 70% of carbapenem-resistant strains contained at least two genes encoding OXA-type carbapenemase. Resistance to carbapenems in the population of Acinetobacter strains in Iran is high, with the majority of isolates showing multidrug resistance. A wide diversity of OXA genes exists among the strains of A. baumannii in Iran. Detection of bla(OXA-51-like) can be used as a simple and reliable method to differentiate A. baumannii strains from other species.
