RESUMO
A novel myovirus, vB_PagM_AAM22 (AAM22), was isolated in Lithuania using Pantoea agglomerans as the host for phage propagation. The 49,744-bp genome of AAM22 has a G + C content of 48.4% and contains 96 probable protein-encoding genes and no genes for tRNA. In total, 34 ORFs were given a putative functional annotation, including genes associated with virion morphogenesis, DNA metabolism, and phage-host interactions. Based on comparative phylogenetic analysis, AAM22 cannot be assigned to any genus currently recognized by the ICTV and is a potential candidate to form a new genus within the family Myoviridae.
Assuntos
Bacteriófagos/isolamento & purificação , Genoma Viral , Myoviridae/isolamento & purificação , Pantoea/virologia , Bacteriófagos/classificação , Bacteriófagos/genética , Composição de Bases , Sequência de Bases , DNA Viral/genética , Myoviridae/classificação , Myoviridae/genética , Fases de Leitura Aberta , FilogeniaRESUMO
Here, we announce the complete genome sequence of the Escherichia coli myophage vB_EcoM_Alf5 belonging to the genus Felixo1virus, whose members have not been comprehensively studied at the molecular level. Phage vB_EcoM_Alf5 infects E. coli K-12-derived laboratory strains and therefore is well suited for functional studies.
