RESUMO
BACKGROUND: Anaplastic large cell lymphoma (ALCL) of the breast capsule is a rare lymphoma involving capsular tissues and/or effusions associated with breast implants. While several studies have detailed the histological and immunohistochemical (IHC) features of these tumors, no study has yet described flow cytometry features of the neoplastic cells of this entity. Here, we report two cases from our institution in which multi-parametric flow cytometry was performed. METHODS: The immunophenotype of ALCL in association with breast implant was evaluated by flow cytometry. RESULTS: We show that much like CD30+ tumor cells of classical Hodgkin lymphoma (CHL) and ALCL of non-breast implant tumors, the neoplastic cells of this entity can be readily identified by flow cytometry. The neoplastic cells of both cases were largely devoid of T-cell antigens, but had expression of weak CD15, strong CD30, and expression of CD40. These results are correlated with routine morphologic and IHC analysis, supporting the flow cytometry immunophenotype. CONCLUSIONS: Flow cytometry can aid in the diagnostic evaluation of effusions or tissue samples in association with breast implant/prostheses.
Assuntos
Implantes de Mama/efeitos adversos , Neoplasias da Mama/diagnóstico , Linfoma Anaplásico de Células Grandes/diagnóstico , Receptores Proteína Tirosina Quinases/deficiência , Linfócitos T/patologia , Adulto , Quinase do Linfoma Anaplásico , Neoplasias da Mama/etiologia , Neoplasias da Mama/metabolismo , Neoplasias da Mama/patologia , Antígenos CD40/genética , Antígenos CD40/metabolismo , Feminino , Citometria de Fluxo , Expressão Gênica , Humanos , Imunofenotipagem , Antígeno Ki-1/genética , Antígeno Ki-1/metabolismo , Antígenos CD15/genética , Antígenos CD15/metabolismo , Linfoma Anaplásico de Células Grandes/etiologia , Linfoma Anaplásico de Células Grandes/metabolismo , Linfoma Anaplásico de Células Grandes/patologia , Pessoa de Meia-Idade , Receptores Proteína Tirosina Quinases/genética , Linfócitos T/metabolismoRESUMO
Background: Anaplastic large cell lymphoma (ALCL) of the breast capsule is a rare lymphoma involving capsular tissues and/or effusions associated with breast implants. While several studies have detailed the histological and immunohistochemical features of these tumors, no study has yet described flow cytometry features of the neoplastic cells of this entity. Here, we report two cases from our institution in which multi-parametric flow cytometry was performed. Methods: The immunophenotype of ALCL in association with breast implant was evaluated by flow cytometry. Results: We show that much like CD30+ tumor cells of classical Hodgkin lymphoma (CHL) and ALCL of non-breast implant tumors, the neoplastic cells of this entity can be readily identified by flow cytometry. The neoplastic cells of both cases were largely devoid of T-cell antigens, but had expression of weak CD15, strong CD30, and expression of CD40. These results are correlated with routine morphologic and immunohistochemical analysis, supporting the flow cytometry immunophenotype. Conclusions: Flow cytometry can aid in the diagnostic evaluation of effusions or tissue samples in association with breast implant/prostheses. © 2014 Clinical Cytometry Society.
RESUMO
A 58-year-old man admitted to the hospital with an acute stroke was found to have a left atrial mass attached to the interatrial septum via a stalk. Although initially thought to be a myxoma, the mass was pathologically proved to be a thrombus. Clues pointing to thrombus as the etiology were a history of atrial fibrillation, the absence of anticoagulation, an enlarged left atrium, and the presence of spontaneous echo contrast.
Assuntos
Átrios do Coração/diagnóstico por imagem , Cardiopatias/complicações , Cardiopatias/diagnóstico por imagem , Acidente Vascular Cerebral/diagnóstico por imagem , Acidente Vascular Cerebral/etiologia , Trombose/complicações , Trombose/diagnóstico por imagem , Diagnóstico Diferencial , Neoplasias Cardíacas/complicações , Neoplasias Cardíacas/diagnóstico por imagem , Humanos , Masculino , Pessoa de Meia-Idade , UltrassonografiaRESUMO
In February 2004, we initiated an epidemiological investigation within a US state to enhance autopsy surveillance for clinically suspected prion disease. During the first 30 months, 30 cases of suspected prion disease were referred from throughout Washington. Of these, 18 cases had prion disease, and all of these were classified as either familial or sporadic Creutzfeldt-Jakob disease (CJD); there was no case of variant CJD. This represents a death rate of approximately 1.1 cases of sporadic CJD per 1 million people per year in Washington. Our results do not support the hypotheses that variant CJD is an emerging illness in Washington or that sporadic CJD is more common in this state than in other regions of the world.
