[Use of a biological model of isolated overload of the skeletal muscle for determination of the effect of anabolic steroids]. / Ispol'zovanie biologicheskoi modeli izolirovannoi peregruzki skeletnoi myshtsy dlia opredeleniia anabolicheskogo effeckta steranabolov.

The authors tested a new biological model for the quantitative determination of the anabolic effect of steranobols of prolonged action. The principle of the method consists in creation of an isolated overload of the skeletal muscles of the shin (m. soleus and. m. plantaris) following section of the tendons of their synergist--m. gastrocnemius in rats, with the subsequent recording their weight gain caused by the administration of anabolic steroid. The method is simple, sensitive and permits to use the animals of different weight and sex without subjecting them to castration.

[Biogenic amines in adaptation of the body to muscular activity]. / Biogennye aminy pri adaptatsii organizma k myshechnoi deiatel'nosti

Alterations in content of histamine, serotonin and in the activity of diamine oxidase from brain, heart auricle, lungs and gastrocnemius muscle were found to have various directions in prolonged training (swimming) of rats. Content of histamine and serotonin was altered in lungs and, especially, in heart muscle; in brain and muscle tissues distinct alterations were not observed. Relative histaminemia was developed in heart auricle and lungs as demonstrated by the increase in content of histamine and by the decrease of the diamine oxidase activity in these tissues. Complete exhaustion of serotonin in heart auricle caused a distinct increase in the histamine-serotonin ratio. High effectiveness of retabolyle was shown in maintenance of histamine and serotonin metabolism under prolonged swimming. But when the animals swam with a load, content of serotonin was decreased in heart auricle even after administration of retabolyle. Adaptation of rats to the prolonged physical exercise (swimming) was apparently related to the compensatory function of an organism that was reflected in the studied mechanisms of trophotropic regulation.

[Catecholamine metabolism during adaptation to muscular activity]. / Obmen katekholaminov pri adaptatsii k myshechnoi deiatel'nosti

In rats, an increased O-methylation in the brain, heart, liver, and in the m. gastrocnemius as well as an increased intensity of oxidative desamination in the liver were revealed after muscular training and administration of retabolile. After additional 70-min swimming, in intact rats the MAO activity was raised in the liver, and the intensity of O-methylation - in the muscle and the heart. In rats adapted to muscular training, no increase of O-methylation in the heart or MAO activity in the liver was observed which may be regarded as an adaptive hypometabolic reaction of the organism for a more "ecomonic" usage of the catecholamines. An increase of the catecholamines contents was revealed in adrenals of the rats after training and retabolile administration. The 70-min swimming obviously suppressed the catecholamines synthesis in the rat adrenals. The synthetic activity of the adrenals in trained animals was also decreased, although insignificantly, which resulted from the preliminary adaptation of the organism to muscular activity.