Bioconcentration and lethal effects of gas-condensate and crude oil on nearshore copepod assemblages.

The progressive establishment of gas platforms and increasing petroleum accidents pose a threat to zooplankton communities and thus to pelagic ecosystems. This study is the first to compare the impacts of gas-condensate and crude oil on copepod assemblages. We conducted microcosm experiments simulating slick scenarios at five different concentrations of gas-condensate and crude oil to determine and compare their lethal effects and the bioconcentration of low molecular weight polycyclic aromatic hydrocarbons (LMW-PAHs) in eastern Mediterranean coastal copepod assemblages. We found that gas-condensate had a two-times higher toxic effect than crude oil, significantly reducing copepod survival with increased exposure levels. The LMW-PAHs bioconcentration factor was 1-2 orders of magnitude higher in copepods exposed to gas-condensate than in those exposed to crude oil. The median lethal concentration (LC50) was significantly lower in calanoids vs. cyclopoid copepods, suggesting that calanoids are more susceptible to gas-condensate and crude oil pollution, with potential trophic implications.

New insights into the impact of leachates from in-field collected plastics on aquatic invertebrates and vertebrates.

The impact of leachates from micronized beached plastics of the Mediterranean Sea and Atlantic Ocean on coastal marine ecosystems was investigated by using a multidisciplinary approach. Chemical analysis and ecotoxicological tests on phylogenetically distant species were performed on leachates from the following plastic categories: bottles, pellets, hard plastic (HP) containers, fishing nets (FN) and rapido trawling rubber (RTR). The bacteria Alivibrio fischeri, the nauplii of the crustaceans Amphibalanus amphitrite and Acartia tonsa, the rotifer Brachionus plicatilis, the embryos of the sea urchin Paracentrotus lividus, the ephyrae of the jellyfish Aurelia sp. and the larvae of the medaka Oryzias latipes were exposed to different concentrations of leachates to evaluate lethal and sub-lethal effects. Thirty-one additives were identified in the plastic leachates; benzophenone, benzyl butyl phthalate and ethylparaben were present in all leachates. Ecotoxicity of leachates varied among plastic categories and areas, being RTR, HP and FN more toxic than plastic bottles and pellets to several marine invertebrates. The ecotoxicological results based on 13 endpoints were elaborated within a quantitative weight of evidence (WOE) model, providing a synthetic hazard index for each data typology, before their integrations in an environmental risk index. The WOE assigned a moderate and slight hazard to organisms exposed to leachates of FN and HP collected in the Mediterranean Sea respectively, and a moderate hazard to leachates of HP from the Atlantic Ocean. No hazard was found for pellet, bottles and RTR. These findings suggest that an integrated approach based on WOE on a large set of bioassays is recommended to get a more reliable assessment of the ecotoxicity of beached-plastic leachates. In addition, the additives leached from FN and HP should be further investigated to reduce high concentrations and additive types that could impact marine ecosystem health.

Cocktail effects of tire wear particles leachates on diverse biological models: A multilevel analysis.

Tire wear particles (TWP) stand out as a major contributor to microplastic pollution, yet their environmental impact remains inadequately understood. This study delves into the cocktail effects of TWP leachates, employing molecular, cellular, and organismal assessments on diverse biological models. Extracted in artificial seawater and analyzed for metals and organic compounds, TWP leachates revealed the presence of polyaromatic hydrocarbons and 4-tert-octylphenol. Exposure to TWP leachates (1.5 to 1000 mg peq L-1) inhibited algae growth and induced zebrafish embryotoxicity, pigment alterations, and behavioral changes. Cell painting uncovered pro-apoptotic changes, while mechanism-specific gene-reporter assays highlighted endocrine-disrupting potential, particularly antiandrogenic effects. Although heavy metals like zinc have been suggested as major players in TWP leachate toxicity, this study emphasizes water-leachable organic compounds as the primary causative agents of observed acute toxicity. The findings underscore the need to reduce TWP pollution in aquatic systems and enhance regulations governing highly toxic tire additives.

Microplastic and mesoplastic pollution in surface waters and beaches of the Canary Islands: A review.

The Canary Archipelago is a group of volcanic islands located in the North Atlantic Ocean with high marine biodiversity. This archipelago intercepts the Canary Current, the easternmost branch of the Azores Current in the North Atlantic Subtropical Gyre, which brings large amounts of litter from remote sources via oceanic transportation. It is, therefore, particularly vulnerable to marine plastic pollution. Here, we present a review of the available studies on mesoplastics and microplastics in the Canary Islands over the last decade to evaluate the level and distribution of plastic pollution in this archipelago. Specifically, we focused on data from beaches and surface waters to assess the pollution level among the different islands as well as between windward and leeward zones, and the main characteristics (size, type, colour, and polymer) of the plastics found in the Canary Islands. The concentrations of meso- and MPs on beaches ranged from 1.5 to 2972 items/m2 with a mean of 381 ± 721 items/m2. The concentration of MPs (>200 µm) in surface waters was highly variable with mean values of 998 × 103 ± 3364 × 103 items/km2 and 10 ± 31 items/m3. Plastic pollution in windward beaches was one order of magnitude significantly higher than in leeward beaches. The accumulation of MPs in surface waters was higher in the leeward zones of the high-elevation islands, corresponding to the Special Areas of Conservation (ZECs) and where the presence of marine litter windrows (MLW) has been reported. Microplastic fragments of polyethylene of the colour category "white/clear/uncoloured" were the most common type of plastic reported in both beaches and surface waters. More studies on the occurrence of MLW in ZECS and plastic pollution in the water column and sediments, including small-size fractions (<200 µm), are needed to better assess the level of plastic pollution and its fate in the Canary Islands. Overall, this review confirms that the Canary Archipelago is a hotspot of oceanic plastic pollution, with concentrations of MPs in surface waters in the highest range reported for oceanic islands and one of the highest recorded mean concentrations of beached meso- and microplastics in the world.

Do weathered microplastics impact the planktonic community? A mesocosm approach in the Baltic Sea.

Microplastics (MPs) are ubiquitous pollutants of increasing concern in aquatic systems. However, little is still known about the impacts of weathered MPs on plankton at the community level after long-term exposure. In this study, we investigated the effects of weathered MPs on the structure and dynamics of a Baltic Sea planktonic community during ca. 5 weeks of exposure using a mesocosm approach (2 m3) mimicking natural conditions. MPs were obtained from micronized commercial materials of polyvinyl chloride, polypropylene, polystyrene, and polyamide (nylon) previously weathered by thermal ageing and sunlight exposure. The planktonic community was exposed to 2 µg L-1 and 2 mg L-1 of MPs corresponding to measured particle concentrations (10-120 µm) of 680 MPs L-1 and 680 MPs mL-1, respectively. The abundance and composition of all size classes and groups of plankton and chlorophyll concentrations were periodically analyzed throughout the experiment. The population dynamics of the studied groups showed some variations between treatments, with negative and positive effects of MPs exhibited depending on the group and exposure time. The abundance of heterotrophic bacteria, pico- and nanophytoplankton, cryptophytes, and ciliates was lower in the treatment with the higher MP concentration than in the control at the last weeks of the exposure. The chlorophyll concentration and the abundances of heterotrophic nanoflagellates, Astromoeba, dinoflagellate, diatom, and metazooplankton were not negatively affected by the exposure to MPs and, in some cases, some groups showed even higher abundances in the MP treatments. Despite these tendencies, statistical analyses indicate that in most cases there were no statistically significant differences between treatments over the exposure period, even at very high exposure concentrations. Our results show that weathered MPs of the studied conventional plastic materials have minimal or negligible impact on planktonic communities after long-term exposure to environmentally relevant concentrations.

Life stage-specific effects of tire particle leachates on the cosmopolitan planktonic copepod Acartia tonsa.

Tire wear particles (TWP) are a major source of microplastics in the aquatic environment and the ecological impacts of their leachates are of major environmental concern. Among marine biota, copepods are the most abundant animals in the ocean and a main link between primary producers and higher trophic levels in the marine food webs. In this study, we determined the acute lethal and sublethal effects of tire particle leachates on different life stages of the cosmopolitan planktonic copepod Acartia tonsa. Median lethal concentration (LC50, 48 h) ranged from 0.4 to 0.6 g L-1 depending on the life stages, being nauplii and copepodites more sensitive to tire particle leachates than adults. The median effective concentration (EC50, 48 h) for hatching was higher than 1 g L-1, indicating a relatively low sensitivity of hatching to tire particle leachates. However, metamorphosis (from nauplius VI to copepodite I) was notably reduced by tire particle leachates with an EC50 (48 h) of 0.23 g L-1 and the absence of metamorphosis at 1 g L-1, suggesting a strong developmental delay or endocrine disruption. Leachates also caused a significant decrease (10-22%) in the body length of nauplii and copepodites after exposure to TWP leachates (0.25 and 0.5 g L-1). We tested a battery of enzymatic biomarkers in A. tonsa adult stages, but a sublethal concentration of 50 mg L-1 of tire particle leachates did not cause a statistically significant effect on the measured enzymatic activities. Our results show that tire particle leachates can negatively impact the development, metamorphosis, and survival of planktonic copepods. More field data on concentrations of TWPs and the fate and persistence of their leached additives is needed for a better assessment of the risk of tire particle pollution on marine food webs.

Tire particles and their leachates reduce the filtration rate of the mussel Mytilus edulis.

Microplastics (MPs) are found in aquatic environments all over the world. Among MPs, tire wear particles (TWPs) are a major contributor to microplastic pollution, and their effects on marine ecosystems are of emerging concern. The blue mussel (Mytilus edulis) is a keystone species in coastal ecosystems with a high risk of exposure to microplastic pollution as the microplastics often overlap in size with the plankton consumed by mussels. In the present study, we investigated the effect of tire particles and their leachates on the filtration rates of M. edulis after short (72 h) and long-term (3 weeks) exposure. Acute exposure to leachates alone causes a significant decrease in the filtration rates of M. edulis with a low observed effect concentration (LOEC) of 1.25 g L-1 and a median effect concentration (EC50) = 3 g L-1. At a concentration of 1.25 g L-1, the filtration rate was reduced compared to the control on average by 38% when mussels were exposed to either TWP or leachates for 72 h. Similarly, mussels exposed to tire particles or their leachates for 3 weeks showed a 46% reduction in filtration rates, compared to the control group. A non-significant difference in filtration rate decrease was found between leachates alone or TWP, which indicates that leachates are the main responsible for the observed toxicity. Our findings indicate that elevated levels of TWP pollution can cause an adverse impact on M. edulis. This could disrupt the natural grazing pressure exerted by M. edulis on phytoplankton, potentially leading to an increased likelihood of algal blooms and hypoxia occurrence in coastal ecosystems.

Effects of petrogenic pollutants on North Atlantic and Arctic Calanus copepods: From molecular mechanisms to population impacts.

Oil and gas industries in the Northern Atlantic Ocean have gradually moved closer to the Arctic areas, a process expected to be further facilitated by sea ice withdrawal caused by global warming. Copepods of the genus Calanus hold a key position in these cold-water food webs, providing an important energetic link between primary production and higher trophic levels. Due to their ecological importance, there is a concern about how accidental oil spills and produced water discharges may impact cold-water copepods. In this review, we summarize the current knowledge of the toxicity of petroleum on North Atlantic and Arctic Calanus copepods. We also review how recent development of high-quality transcriptomes from RNA-sequencing of copepods have identified genes regulating key biological processes, like molting, diapause and reproduction in Calanus copepods, to suggest linkages between exposure, molecular mechanisms and effects on higher levels of biological organization. We found that the available ecotoxicity threshold data for these copepods provide valuable information about their sensitivity to acute petrogenic exposures; however, there is still insufficient knowledge regarding underlying mechanisms of toxicity and the potential for long-term implications of relevance for copepod ecology and phenology. Copepod transcriptomics has expanded our understanding of how key biological processes are regulated in cold-water copepods. These advances can improve our understanding of how pollutants affect biological processes, and thus provide the basis for new knowledge frameworks spanning the effect continuum from molecular initiating events to adverse effects of regulatory relevance. Such efforts, guided by concepts such as adverse outcome pathways (AOPs), enable standardized and transparent characterization and evaluation of knowledge and identifies research gaps and priorities. This review suggests enhancing mechanistic understanding of exposure-effect relationships to better understand and link biomarker responses to adverse effects to improve risk assessments assessing ecological effects of pollutant mixtures, like crude oil, in Arctic areas.

Crude Oil and Its Burnt Residues Induce Metamorphosis in Marine Invertebrates.

Metamorphosis is a critical process in the life cycle of most marine benthic invertebrates, determining their transition from plankton to benthos. It affects dispersal and settlement and therefore decisively influences the dynamics of marine invertebrate populations. An extended period of metamorphic competence is an adaptive feature of numerous invertebrate species that increases the likelihood of finding a habitat suitable for settlement and survival. We found that crude oil and residues of burnt oil rapidly induce metamorphosis in two different marine invertebrate larvae, a previously unknown sublethal effect of oil pollution. When exposed to environmentally realistic oil concentrations, up to 84% of tested echinoderm larvae responded by undergoing metamorphosis. Similarly, up to 87% of gastropod larvae metamorphosed in response to burnt oil residues. This study demonstrates that crude oil and its burned residues can act as metamorphic inducers in marine planktonic larvae, short-circuiting adaptive metamorphic delay. Future studies on molecular pathways and oil-bacteria-metamorphosis interactions are needed to fully understand the direct or indirect mechanisms of oil-induced metamorphosis in marine invertebrates. With 90% of chronic oiling occurring in coastal areas, this previously undescribed impact of crude oil on planktonic larvae may have global implications for marine invertebrate populations and biodiversity.

Lethal effect of leachates from tyre wear particles on marine copepods.

With thousands of tons of Tyre Wear Particles (TWP) entering the aquatic environment every year, TWP are considered a major contributor to microplastic pollution. TWP leach organic compounds and metals in water, potentially affecting the marine food web. However, little is known about the toxicity of TWP leachates on marine copepods, a major food web constituent, and a key group to determine the environmental risk of pollution in marine ecosystems. In this study, we determined the lethal effect of TWP leachates on marine copepods after 24, 48, and 72-h of exposure to 0.05-100% leachate solutions prepared using a concentration of 5 g TWP L-1. The calanoids Acartia tonsa, Temora longicornis and Centropages hamatus, the cyclopoid Oithona davisae and the harpacticoid Amonardia normanni were used as experimental species. TWP leachates were toxic to all the studied species, with toxicity increasing as leachate solution and exposure time increased. Median lethal concentration (LC50, 72-h) ranged from 0.22 to 3.43 g L-1 and calanoid copepods were more sensitive to TWP leachates than the cyclopoid O. davisae and the harpacticoid A. normanni. Toxicity of TWP leachates was not related to the copepod body size, which suggests that other traits such as foraging behaviour or adaptation to contaminants could explain the higher tolerance of cyclopoid and harpacticoid to TWP leachates compared to calanoid copepods. Although field data on the concentration of TWP and their chemical additives are still limited, our results suggest that TWP leachates can negatively impact planktonic food webs in coastal areas after road runoff events.

Toxicity of tire particle leachates on early life stages of keystone sea urchin species.

Particles from tires are a major fraction of microplastic pollution. They contain a wide range of chemical additives that can leach into the water and be harmful to aquatic organisms. In this study, we investigated the acute toxicity of tire particle leachates in early life stages of three keystone echinoderm species (Paracentrotus lividus, Arbacia lixula, Diadema africanum). Embryos were exposed for 72 h to a range of leachate dilutions, prepared using a concentration of 1 g L-1. Larval growth, abnormal development, and mortality were the measured endpoints. Furthermore, we estimated the activity of glutathione S transferase (GST) and the electron transport system (ETS) in P. lividus. Strong concentration-dependent responses were observed in all species, though with differing sensitivity. The median effect concentrations for abnormal development in P. lividus and A. lixula were 0.16 and 0.35 g L-1, respectively. In D. africanum, mortality overshadowed abnormal development and the median lethal concentration was 0.46 g L-1. Larvae of P. lividus were significantly smaller than the control from 0.125 g L-1, while the other two species were affected from 0.5 g L-1. ETS activity did not change but there was a non-significant trend of increasing GST activity with leachate concentration in P. lividus. Seven organic chemicals and eight metals were detected at elevated concentrations in the leachates. While we regard zinc as a strong candidate to explain some of the observed toxicity, it can be expected that tire particle leachates exhibit a cocktail effect and other leached additives may also contribute to their toxicity. Our results emphasize the importance of multi-species studies as they differ in their susceptibility to tire particle pollution. We found negative effects at concentrations close to projections in the environment, which calls for more research and mitigation actions on these pollutants.

Is Zooplankton an Entry Point of Microplastics into the Marine Food Web?

Microplastics (MPs) overlap in size with phytoplankton and can be ingested by zooplankton, transferring them to higher trophic levels. Copepods are the most abundant metazoans among zooplankton and the main link between primary producers and higher trophic levels. Ingestion of MPs has been investigated in the laboratory, but we still know little about the ingestion of MPs by zooplankton in the natural environment. In this study, we determined the concentration and characteristics of MPs down to 10 µm in zooplankton samples, sorted calanoid copepods, and fecal pellets collected in the Kattegat/Skagerrak Sea (Denmark). We found a median concentration of 1.7 × 10-3 MPs ind-1 in the zooplankton samples, 2.9 × 10-3 MPs ind-1 in the sorted-copepods, and 3 × 10-3 MPs per fecal pellet. Most MPs in the zooplankton samples and fecal pellets were fragments smaller than 100 µm, whereas fibers dominated in the sorted copepods. Based on the collected data, we estimated a MP budget for the surface layer (0-18 m), where copepods contained only 3% of the MPs in the water, while 5% of the MPs were packed in fecal pellets. However, the number of MPs exported daily to the pycnocline via fecal pellets was estimated to be 1.4% of the total MPs in the surface layer. Our results indicate that zooplankton are an entry point of small MPs in the food web, but the number of MPs in zooplankton and their fecal pellets was low compared with the number of MPs found in the water column and the occurrence and/or ingestion of MPs reported for nekton. This suggests a low risk of MP transferring to higher trophic levels through zooplankton and a quantitatively low, but ecologically relevant, contribution of fecal pellets to the vertical exportation of MPs in the ocean.

Searching for hotspots of neustonic microplastics in the Canary Islands.

In this study, we investigated the concentration, distribution, and characteristics of neustonic MPs in the Canary Islands, with a particular focus on the island leeward zones, where a high accumulation of floating marine microplastics is expected. Samples were collected with a manta net at 15 different sites from Alegranza to La Gomera during the IMPLAMAC expedition. The microplastic concentration in surface waters ranged from 0.27 MPs/m3 in Alegranza to 136.7 MPs/m3 in the south of Gran Canaria. The highest concentration of MPs found was due to the presence of a sea-surface slick, also called "marine litter windrow", formed in the south of Gran Canaria. The most abundant zooplankton group in the neuston was copepods, except at the marine litter windrow where fish larvae and eggs predominated. This indicates that coastal areas where marine litter windrows are formed have a high risk of MP ingestion and potential adverse effects on biota.

A protocol for lixiviation of micronized plastics for aquatic toxicity testing.

Plastics contain various types and amounts of additives that can leach into the water column when entering aquatic ecosystems. Some leached plastic additives are hazardous to marine biota at environmentally relevant concentrations. Disparate methodological approaches have been adopted for toxicity testing of plastic leachates, making comparison difficult. Here we propose a protocol to standardize the methodology to obtain leachates from microplastics (MPs) for aquatic toxicity testing. Literature reviewing and toxicity tests using marine model organisms and different types of MPs were conducted to define the main methodological aspects of the protocol. Acute exposure to leachates from the studied plastics caused negative effects on the early life stages of sea urchins and marine bacteria. We provide recommendations of key factors influencing lixiviation of MPs , such as particle size (<250 µm), solid-to-liquid ratio (1-10 g/L), mixing conditions (1-60 rpm), and lixiviation time (72 h). The proposed methodology was successful to determine the toxicity of leachates from different micronized plastics on marine biota. Our recommendations balance sensitivity, feasibility and environmental relevance, and their use would help ensure comparability amongst studies for a better assessment of the toxicity of plastic leachates on aquatic biota.

Abundance and distribution of microplastics in surface waters of the Kattegat/ Skagerrak (Denmark).

Microplastics (MPs) are ubiquitous pollutants in the ocean, and there is a general concern about their persistence and potential effects on marine ecosystems. We still know little about the smaller size-fraction of marine MPs (MPs <300 µm), which are not collected with standard nets for MPs monitoring (e.g., Manta net). This study aims to determine the concentration, composition, and size distribution of MPs down to 10 µm in the Kattegat/Skagerrak area. Surface water samples were collected at fourteen stations using a plastic-free pump-filter device (UFO sampler) in October 2020. The samples were treated with an enzymatic-oxidative method and analyzed using FPA-µFTIR imaging. MPs concentrations ranged between 11 and 87 MP m-3, with 88% of the MPs being smaller than 300 µm. The most abundant shape of MPs were fragments (56%), and polyester, polypropylene, and polyethylene were the dominant synthetic polymer types. The concentration of MPs shows a significant positive correlation to the seawater density. Furthermore, there was a tendency towards higher MPs concentrations in the Northern and the Southern parts of the study area. The concentration of MPs collected with the UFO sampler was several orders of magnitude higher than those commonly found in samples collected with the Manta net due to the dominance of MP smaller size fractions. Despite the multiple potential sources of MPs in the study area, the level of MPs pollution in the surface waters was low compared (<100 MP m-3) to other regions. The concentrations of MPs found in the studied surface waters were six orders of magnitude lower than those causing negative effects on pelagic organisms based on laboratory exposure studies, thus is not expected to cause any impact on the pelagic food web.

The Behavior of Planktonic Copepods Minimizes the Entry of Microplastics in Marine Food Webs.

The entry of microplastics (MPs) into marine food webs is a major environmental concern. We investigated how the behavior of planktonic copepods influences the risk of MPs to enter marine food webs by applying a trait-based approach and by combining experiments (bottle incubations and video observations) with biogeographical analyses. We aimed to evaluate which type of feeding behavior is most risky in terms of MP ingestion and which marine geographical areas are more susceptible to MP ingestion by planktonic copepods. We used different species as models of the main foraging behaviors in planktonic copepods: feeding-current, cruising, ambush, and mixed behavior feeding. All behaviors showed a similarly low risk of MP ingestion, up to 1 order of magnitude lower than for similar-sized microalgae. We did not observe any influence of the prey type or MP size (8 and 20 µm) on MP ingestion for any of the behaviors. By mapping the global distribution of feeding behaviors, we showed that feeding-current feeding is the most common behavior, but the risk of MP ingestion remains equally low across the global ocean, independently of the predominant behavior. Overall, our results suggest a low risk of MP ingestion by planktonic copepods and therefore a minimal risk of trophic transfer of MPs via marine pelagic copepods in marine ecosystems.

Toxicity of tyre wear particle leachates to marine phytoplankton.

Tyre wear particles (TWP) are some of the dominant sources of microplastics in the aquatic environment. Once TWP enter aquatic systems, they can leach certain plastic additives that can be potentially toxic to biota. However, little is known about the impact of TWP lixiviates on marine phytoplankton, the base of marine food webs. This study aims to determine the acute toxic effect of leachates derived from TWP on three phytoplankton species: the cryptophyte Rhodomonas salina, the diatom Thalassiosira weissflogii and the dinoflagellate Heterocapsa steinii, using the median effect concentration (EC50) for specific growth rate as endpoint. Leachates were obtained by incubating 1 g L-1 of < 250 µm TWP in artificial seawater for 3 days. Each phytoplankton species was exposed to leachates at five different concentrations, and cell concentrations were measured every 24 h over 3 days. Leachates from TWP were toxic to marine phytoplankton. The dinoflagellate H. steinii was the most sensitive species, with 72-h EC50 of 23% leachate concentration, whereas R. salina and T. weissflogii exhibited EC50 values of 64% and 73%, respectively. Our results suggest that TWP leachates have a negative effect on phytoplankton growth, although more field data on the concentration of TWPs and their leachates is needed to fully evaluate the environmental impact of TWP.

Unpalatable Plastic: Efficient Taste Discrimination of Microplastics in Planktonic Copepods.

Planktonic copepods are the most abundant animals in the ocean and key players in global biochemical processes. Recent modeling suggests that zooplankton ingestion of microplastics (MPs) can disrupt the biological carbon pump and accelerate a global loss of oceanic oxygen. Here we investigate the behavioral responses and ingestion rates of a model feeding-current generating copepod when exposed to microplastics of different characteristics by small-scale video observations and bottle incubations. We found that copepods rejected 80% of the microplastics after touching them with their mouth parts, in essence exhibiting a kind of taste discrimination. High rejection rates of microplastics were independent of polymer type, shape, presence of biofilms, or sorbed pollutant (pyrene), indicating that microplastics are unpalatable for feeding-current feeding copepods and that post-capture taste discrimination is a main sensorial mechanism in the rejection of microplastics. In an ecological context, taking into account the behaviors of planktonic copepods and the concentrations of microplastics found in marine waters, our results suggest a low risk of microplastic ingestion by zooplankton and a low impact of microplastics on the vertical exportation of fecal pellets.

Ingestion and impact of microplastics on arctic Calanus copepods.

Microplastics (MPs) are contaminants of emerging concern in the Arctic, but knowledge of their potential effects on Arctic plankton food webs remains scarce. We experimentally investigated ingestion and effects of MPs (20 µm polyethylene spheres) on the arctic copepods Calanus finmarchicus, C. glacialis and C. hyperboreus. These species dominate arctic zooplankton biomass and are relevant target species to investigate the potential impacts of MPs on the Arctic marine ecosystem. Females of each species were exposed to two concentrations of MPs (200 and 20,000 MPs L-1) in combination with different food (diatom) concentrations, reflecting high (3000-5000 cells mL-1, spring phytoplankton bloom) and low (50-500 cells mL-1, pre/post bloom) food conditions. MPs did not affect negatively fecal pellet production rates in any of the species at the studied exposure concentrations. However, egg production rates of copepods exposed to MPs were 8 times higher compared with the controls, which suggests that MP exposure can cause stress-induced spawning in arctic copepods. Microscopic examination of the fecal pellets confirmed ingested MPs in the three species (up to aprox. 1000 MPs cop-1 d-1). The number of MPs per pellet decreased exponentially with increasing food concentration. The daily ingestion of MPs per copepod was higher at low- food concentrations (250-500 cells mL-1). At our exposure conditions, the presence of MPs inside C. hyperboreus fecal pellets did not affect their sinking rates. Overall, our experimental research show that 1) acute exposure to virgin polyethylene MPs has a low impact on arctic Calanus species at environmentally relevant MP concentrations, independent of food availability, and 2) arctic copepods influence the environmental fate of plankton-sized MPs by exporting buoyant MPs from the surface layer to the sea floor via fecal pellets.

Quantification of plankton-sized microplastics in a productive coastal Arctic marine ecosystem.

Microplastics (MPs) are polluting the Arctic, but our understanding of their abundance, distribution, and sources is limited. This study quantified MPs down to 10 µm in marine waters of the most populated region in Greenland. A new plastic-free pump-filter system was used to collect MPs from surface waters in the fjord Nuup Kangerlua close to Nuuk. Additionally, we took samples by horizontal tows with a bongo net (300 µm mesh-size). The median concentrations were 142 MPs m-3 and 0.12 MPs m-3 in the pump and bongo samples, respectively. The most abundant polymer was polyester across stations and sampling types. Fibers were the dominant shape in the bongo samples, while non-fibrous particles dominated in the pump samples. MP abundance was lower in the fjord and increased close to Nuuk and towards the open ocean, indicating that Nuuk is an important point source for MPs. In both samples, concentrations of MPs increased with decreasing size, illustrating the importance of quantifying the smallest fraction of MPs. Thus, the use of methods allowing for a quantification of the smallest MPs is vital to reduce the underestimation of MP concentrations in the environment. The smallest size fraction is also most available to plankton-feeding marine invertebrates and an important entry point for MPs into marine food webs. At the found concentrations, immediate adverse effects on the pelagic food webs are unlikely. However, growing anthropogenic activities could increase the risk of MPs to affect the sensitive Arctic ecosystem.