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1.
Genet Mol Res ; 14(4): 18160-71, 2015 Dec 28.
Artigo em Inglês | MEDLINE | ID: mdl-26782463

RESUMO

This study investigated the effects of hyperbaric oxygen therapy (HBOT) and dimethyl sulfoxide (DMSO) in tissue necrosis, genotoxicity, and cell apoptosis. Random skin flaps were made in 50 male Wistar rats, randomly divided into the following groups. Control group (CT), wherein a rectangular skin section (2 x 8 cm) was dissected from the dorsal muscle layer, preserving the cranial vessels, lifted, and refixed to the bed; distilled water (DW) group, in which DW was injected into the distal half of the skin flap; DMSO group, wherein 5% DMSO was injected; HBOT group, comprising animals treated only with HBOT; and HBOT + DMSO group, comprising animals treated with 100% oxygen at 2.5 atmospheres absolute for 1 h, 2 h after the experiment, daily for 10 consecutive days. A skinflap specimen investigated by microscopy. The percentage of necrosis was not significantly different between groups. The cell viability index was significantly different between groups (P < 0.001): 87.40% (CT), 86.20% (DW), 84.60% (DMSO), 86.60% (DMSO + HBO), and 91% (HBO) (P < 0.001), as was the cell apoptosis index of 12.60 (CT), 12.00 (DW), 15.40 (DMSO), 9.00 (HBO), and 12.00 (DMSO + HBO) (P < 0.001). The genotoxicity test revealed the percentage of cells with DNA damage to be 22.80 (CT), 22.60 (DW), 26.00 (DMSO), 8.80 (DMSO + HBO), and 7.20 (HBO) (P < 0.001). Although the necrotic area was not different between groups, there was a significant reduction in the cellular DNA damage and apoptosis index in the HBOT group.


Assuntos
Dimetil Sulfóxido/administração & dosagem , Oxigenoterapia Hiperbárica , Necrose/terapia , Retalhos Cirúrgicos/patologia , Animais , Apoptose/efeitos dos fármacos , Sobrevivência Celular/efeitos dos fármacos , Dano ao DNA/efeitos dos fármacos , Humanos , Masculino , Necrose/patologia , Ratos , Pele/efeitos dos fármacos , Pele/patologia
2.
Arq. bras. med. vet. zootec ; 55(1): 123-125, Feb. 2003. tab
Artigo em Inglês | LILACS | ID: lil-332819

RESUMO

A coleta de Diptera em fezes bovinas depositadas nas pastagens foi conduzida de janeiro a agosto de 2001 em Itumbiara, Goiás. As fezes foram expostas nas pastagens por 24, 48, 72, 96, 120, 144, 168, 192, 216 e 240 horas e posteriormente levadas para laboratório para extraçäo e separaçäo dos dípteros. Um total de 100 fezes bovinas foram expostas nas pastagens, das quais 3099 dípteros muscóides foram coletados. As espécies mais abundantes foram: Palaeosepsis spp. e Sarcophagula occidua. A maioria dos exemplares foi coletada em fezes bovinas expostas nos períodos de 72 e 144 horas no campo


Assuntos
Animais , Bovinos , Bovinos , Dípteros , Fezes
3.
Arq. bras. med. vet. zootec ; 54(4): 454-456, jul.-ago. 2002. tab
Artigo em Português | LILACS | ID: lil-328426

RESUMO

This work reports the occurrence of microhimenopterous in cattle dung in Itumbiara, Goiás, Brazil. Cattle dung pats were exposed on a pasture for 24, 48, 72, 96, 120, 144, 168, 192, 216 and 240 hours and were then separately taken to the laboratory for artropods extraction. A total of 100 dung pats were exposed at pasture. The pupae were individually placed in transparent gelatin capsules until the emergence of the parasitoids. The main species of parasitoids collected from bovine dung were Paraganapis egeria and Spalangia drosophilae


Assuntos
Parasitos , Controle Biológico de Vetores
4.
Arq. bras. med. vet. zootec ; 53(4): 449-450, ago. 2001.
Artigo em Português | LILACS | ID: lil-307718

RESUMO

Triplasta coxalis (Ashmeda) (Figitidae: Eucolinae), was found as a parasitoid of Palaeosepsis sp. (Diptera: Sepsidae) in Itumbiara, GO, Brazil (18§25'S and 49§13'W). The samples of bovine dung were taken from pats 24, 36, 72, 96, 120, 144, 168, 192, 216 and 240 hours of exposittion, placed in plastic containers and kept in the laboratory conditions. The pupae were extracted by flotation in water. Pupae were individually placed in gelatin capsules until emergence of adult flies or their parasitoids. Four and one specimes of Triplasma coxalis and Kleidotoma nigra, respectively, were collected. This was the first report on occurrence of the parasitoid Kleidotoma nigra and Triplasta coxalis in Goiás


Assuntos
Dípteros , Fezes , Parasitologia , Bovinos , Interações Hospedeiro-Parasita
5.
Rev Saude Publica ; 34(5): 545-6, 2000 Oct.
Artigo em Português | MEDLINE | ID: mdl-11105121

RESUMO

The first occurrence of the parasitoid Pachycrepoideus vindemiae on pupae of Ophyra aenescens, a fly of medical-sanitary importance, is reported. A swine carcass was used as bait to collect the insects. In the study, 302 pupae of Ophyra aenescens (Wiedemann) (Diptera: Muscidae) were obtained, 6 (1.98%) of them yielded the parasitoid Pachycrepoideus vindemiae (Hymenoptera: Pteromalidae


Assuntos
Dípteros/fisiologia , Himenópteros/parasitologia , Animais , Controle de Insetos , Insetos Vetores/fisiologia , Suínos
6.
Arq. bras. med. vet. zootec ; 52(5): 459-60, out. 2000. tab
Artigo em Português | LILACS | ID: lil-285597

RESUMO

Muscoid dipterous insects associated with pig carcass and their parasitoids collected in pasture and wood were collected from savanna (cerrado) at Goiás, Brazil. The species collected more often were: Chrysomya albiceps (89.5 per cent ), Ophyra sp. (6.9 per cent) (flies) and Pachycrepoideus vindemiae (33.3 per cent) and Spalangia endius (38.8 per cent) (parasitoids). The parasitism rate was 0.4 per cent


Assuntos
Animais , Dípteros/fisiologia , Dípteros/parasitologia , Suínos
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