Frequencies of genetic variants of the Rh, Kell, Duffy, Kidd, MNS and Diego systems of northwest Rio Grande do Sul, Brazil

ABSTRACT Introduction: To date, 340 antigen-organized 43 blood group systems are recognized, being ABO, Rh, Kell, Duffy, Kidd, MNS and Diego the most clinically relevant. The aim of this study was to assess the distribution of alleles and genotypes of the blood group systems Rh, Kell, Duffy, Kidd, MNS and Diego in 810 blood donors registered in the hemotherapy unit in northwest Rio Grande do Sul, Brazil Methods: We evaluated the genetic variability of blood groups Rh (c.676G>C and c.307C>T), Kell (c.578C>T), Kidd (c.838A>G), Duffy (c.125A>G and c.l-67T>C), Diego (c.2561C>T) and MNS (c.143T>C) in 810 volunteer blood donors of Rio Grande do Sul, southern Brazil. The genetic profiling was performed through allelic discrimination assays using hydrolysis probes (TaqMan®) real-time PCR system. Results: The most frequent blood group genotypes found in our study population were: RHC*Cc (51.5%), RHC*ee (70.1%), FY*A/FY*B (49.3%), GATA -67T/T (93.5%), KEL*2/KEL*2 (93.4%), Jk*A/JK*B (53.2%) and DI*02/DI*02 (95.4%). Some statistical differences were observed on comparing the population of this study with populations from other states in Brazil, mainly with population of Minas Gerais, Bahia and Paraná, which showed some differences from the population of Porto Alegre, which was more similar to those of Santa Catarina and São Paulo Conclusion: The frequency of red blood cell polymorphisms in our study is different from that of blood donors in other regions of Brazil. The results showed the importance of extended genotyping in adequate blood screening and the existence of rare genotypes in Brazilian regular blood donors

Efeito da orientação pré-operatória por grupo multiprofissional na ansiedade de pacientes submetidos à cirurgia cardíaca / Effect of orientations pre-operative by multiprossional group on anxiety of patients undergoing cardiac surgery

Objetivo: comparar o nível de ansiedade em pacientes no pré-operatório de cirurgia cardíaca, antes e após as orientações recebidas em grupo, por uma equipe multiprofissional. Método: estudo com delineamento de coorte. Foram selecionados, consecutivamente, pacientes submetidos à cirurgia de revascularização do miocárdio e troca valvar. O instrumento utilizado para coleta dos dados foi o inventário de ansiedade traço-estado (IDATE). Este instrumento foi aplicado, antes e após as orientações fornecidas em grupo, pela equipe multiprofissional, com posterior comparação das médias. Resultados: a amostra estudada foi composta por 20 pacientes, sendo a maioria (60%) do sexo masculino, com idade média de 58 ±15,7 anos. A média de ansiedade-traço foi de 46,5 ±9,43, e ansiedade-estado de 43,5 ±12,8 antes da orientação e, após orientação, a média de ansiedade-estado foi de 40,15 ±10,06 (p=0,020). Conclusão: o nível de ansiedade dos pacientes reduziu significativamente, comparando-se antes e após as orientações pré-operatórias em grupo.

Aim: to compare the level of anxiety in patients undergoing cardiac surgery before and after the multi-professional team intervention. Method: this is a cohort study which patients undergoing coronary artery bypass grafting and valve replacement surgeries. The State-Trait Anxiety Inventory (STAI) was used for data collection. This instrument was administered before and after the multi-professional team intervention with subsequent comparison of means. Results: the sample with 20 patients was composed mostly (60%) by male subjects with mean age 58 ±15.7 years. Before intervention, the trait anxiety mean was 46.5 ±9.43, and 43.5 ±12.8 for state anxiety; after intervention, state anxiety mean was 40.15 ±10.06 (p = 0.020). Conclusion: the anxiety level in patients has significantly decreased when before and after moments are compared.

Objetivo: comparar el nivel de ansiedad en los pacientes del preoperatorio de la cirugía cardiaca, antes y después de las orientaciones recibidas en grupo por uno equipo multiprofesional. Método: estudio con diseño de corte. Fueron seleccionados pacientes sometidos a cirugía de revascularización coronaria y reemplazo de la válvula. El instrumento utilizado para la recolección de datos fue el Inventario Ansiedad IDATE. Este instrumento fue aplicado antes y después de las directrices establecidas en el grupo por el equipo profesional con la posterior comparación de medias. Resultados: la muestra del estudio consistió 20 pacientes, su mayoría (60%) hombres, edad media 58 ±15,7 años. La media de ansiedad-rasgo fue 46,5 ±9,43 y ansiedad-estado 43,5 ±12,8 antes y después de la orientación, la media fue 40,15 ±10,06 (p = 0,020) ansiedad-estado. Conclusión: el nivel de ansiedad de los pacientes disminuyó significativamente, comparando antes y después de las directrices preoperatorias del grupo.