Energy-dependent effect trial of photobiomodulation on blood pressure in hypertensive rats.

The main purpose of this work was to construct an energy-dependent response curve of photobiomodulation on arterial pressure in hypertension animal model. To reach this objective, we have used a two-kidney one clip (2K-1C) rat model. Animals received acute laser light irradiation (660 nm) on abdominal region using different energy (0.6, 1.8, 3.6, 7.2, 13.8, 28.2, 55.8, and 111.6 J), the direct arterial pressure was measured by femoral cannulation, and systolic arterial pressure (SAP), diastolic arterial pressure (DAP), heart rate (HR), and time of effect were obtained. Our results indicated that 660 nm laser light presents an energy-dependent hypotensive effect, and 28.2 J energy irradiation reached the maximum hypotensive effect, inducing a decreased SAP, DAP, and HR (decrease in SAP: - 19.23 ± 1.82 mmHg, n = 11; DAP: - 9.57 ± 2.23 mmHg, n = 11; HR: - 39.15 ± 5.10 bpm, n = 11; and time of hypotensive effect: 3068.00 ± 719.00 s, n = 11). The higher energy irradiation evaluated (111.6 J) did not induce a hypotensive effect and induced an increase in HR (21.69 ± 7.89 bpm, n = 7). Taken together, our results indicate that red laser energy irradiation from 7.2 to 55.8 J is the effective therapeutic window to reduce SAP, DAP, MAP, and HR and induce a long-lasting hypotensive effect in rats, with effect loss at higher energy irradiation (111.6 J).

Comparison of the low level laser therapy effects on cultured human gingival fibroblasts proliferation using different irradiance and same fluence.

BACKGROUND AND OBJECTIVE: The low level laser therapy (LLLT) has been used in Dentistry to improve wound healing. In order to analyse the effect of LLLT on the in vitro proliferation of gingival fibroblasts we developed a primary culture of human gingival fibroblasts. STUDY DESIGN/MATERIALS AND METHODS: The cell line named LMF was grown in Dulbecco's Modified Eagle's medium (DME) with either 5% (nutritional deficit) or 10% fetal bovine serum (fbs). Laser irradiation was carried out with diode lasers with the following wavelengths: 670 nm (L1), 780 nm (L2), 692 nm (L3), and 786 nm (L4). The fluence was fixed in 2 J/cm(2). For growth analysis, control (not irradiated) and treated cultures (irradiated) were plated in 60 mm diameter culture dishes for 12 h before the irradiation. RESULTS: We found that cells cultured in nutritional deficit condition grown in medium supplemented by only 5% fbs presented a cell proliferation rate significantly smaller that cell grown in ideal culture conditions (10% fbs). However, when irradiated, cells in nutritional deficit presented cell growth similar or higher than that of control cells grown in ideal culture conditions. Using the same fluence, the infrared laser induced a higher cell proliferation than visible laser when the power outputs were different. However, lasers of equal power output presented similar effect on cell growth independently of their wavelengths. CONCLUSIONS: The LLLT acts by improving the in vitro fibroblast proliferation and a smaller laser exposure time results in higher proliferation.