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1.
J Appl Toxicol ; 44(8): 1129-1138, 2024 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-38567776

RESUMO

Rubus imperialis Chum. Schl. (Rosaceae) have demonstrated some pharmacological activities, including gastroprotective action. However, genotoxic effects of R. imperialis extract was also reported. Since niga-ichigoside F1 (NIF1) is a major compound of this plant species, and which has proven pharmacological properties, it is essential to investigate whether this compound is responsible for the observed toxicity. Therefore, the objective of this study was to analyze the effects of NIF1 on HepG2/C3A cells for possible cytogenotoxicity, cell cycle and apoptosis influence, and expression of genes linked to the DNA damage, cell cycle, cell death, and xenobiotic metabolism. The results showed no cytogenotoxic effects of NIF1 at concentrations between 0.1 and 20 µg/ml. Flow cytometry also showed no cell cycle or apoptosis disturbance. In the gene expression analysis, none of the seven genes investigated showed altered expression. The data indicate that NIF1 has no cytogenotoxic effects, and no interruption of the cell cycle, or induction of apoptosis, apparently not being responsible for the cytotoxic effects observed in the crude extract of R. imperialis.


Assuntos
Apoptose , Ciclo Celular , Humanos , Células Hep G2 , Apoptose/efeitos dos fármacos , Ciclo Celular/efeitos dos fármacos , Rubus/química , Dano ao DNA/efeitos dos fármacos , Extratos Vegetais/toxicidade , Extratos Vegetais/farmacologia , Sobrevivência Celular/efeitos dos fármacos , Relação Dose-Resposta a Droga , Saponinas/toxicidade , Saponinas/farmacologia
2.
Medwave ; 23(11): e2713, 2023 Dec 07.
Artigo em Inglês, Espanhol | MEDLINE | ID: mdl-38079562

RESUMO

Pain is a major public health concern associated with emotional disorders and physical disability and generates increased sick leave and healthcare expenditures. However, despite its social impact and the high pain-related costs, the training of healthcare professionals, such as physical therapists, in pain management is insufficient, leading to an inadequate therapeutic approach. This study aimed to determine the existence of a specific program on pain within the curricula of physical therapy careers in accredited universities in Chile. Out of 40 physical therapy careers, we analyzed 38 curricula. None of them had a specific discipline on pain management in their curricula. Although there is no concrete definition of the best modality for pain education in undergraduate students, it has been shown that following the International Association for The Study of Pain curricula improves students' knowledge and awareness of pain management.


El dolor es un importante problema de salud pública, asociado con trastornos emocionales y discapacidad física, generando un aumento en licencias laborales y gastos en el sistema de salud. Sin embargo, a pesar del impacto social y los altos costos relacionados con el dolor, hay una capacitación insuficiente en la formación de los profesionales de la salud, como kinesiólogos y fisioterapeutas sobre el manejo del dolor, lo que lleva a un abordaje terapéutico inadecuado. El objetivo de este estudio fue determinar la existencia de un programa específico en dolor dentro de las mallas curriculares de las carreras de kinesiología en universidades acreditadas en Chile. De 40 carreras de kinesiología, analizamos 38 mallas curriculares. Ninguna de ellas presentaba una disciplina específica sobre dolor en su plan de estudios. Si bien no hay una definición concreta sobre la mejor modalidad para la educación en dolor en alumnos de pregrado, se ha demostrado que seguir los planes de estudios de la impacta significativamente en el conocimiento y las creencias del dolor de los estudiantes.


Assuntos
Currículo , Dor , Humanos , Chile , Dor/etiologia , Escolaridade , Modalidades de Fisioterapia
3.
Medwave ; 23(11)31-12-2023.
Artigo em Inglês, Espanhol | LILACS-Express | LILACS | ID: biblio-1524726

RESUMO

El dolor es un importante problema de salud pública, asociado con trastornos emocionales y discapacidad física, generando un aumento en licencias laborales y gastos en el sistema de salud. Sin embargo, a pesar del impacto social y los altos costos relacionados con el dolor, hay una capacitación insuficiente en la formación de los profesionales de la salud, como kinesiólogos y fisioterapeutas sobre el manejo del dolor, lo que lleva a un abordaje terapéutico inadecuado. El objetivo de este estudio fue determinar la existencia de un programa específico en dolor dentro de las mallas curriculares de las carreras de kinesiología en universidades acreditadas en Chile. De 40 carreras de kinesiología, analizamos 38 mallas curriculares. Ninguna de ellas presentaba una disciplina específica sobre dolor en su plan de estudios. Si bien no hay una definición concreta sobre la mejor modalidad para la educación en dolor en alumnos de pregrado, se ha demostrado que seguir los planes de estudios de la impacta significativamente en el conocimiento y las creencias del dolor de los estudiantes.


Pain is a major public health concern associated with emotional disorders and physical disability and generates increased sick leave and healthcare expenditures. However, despite its social impact and the high pain-related costs, the training of healthcare professionals, such as physical therapists, in pain management is insufficient, leading to an inadequate therapeutic approach. This study aimed to determine the existence of a specific program on pain within the curricula of physical therapy careers in accredited universities in Chile. Out of 40 physical therapy careers, we analyzed 38 curricula. None of them had a specific discipline on pain management in their curricula. Although there is no concrete definition of the best modality for pain education in undergraduate students, it has been shown that following the International Association for The Study of Pain curricula improves students' knowledge and awareness of pain management.

4.
J Appl Toxicol ; 43(2): 323-334, 2023 02.
Artigo em Inglês | MEDLINE | ID: mdl-36000810

RESUMO

3-(3,4-Dihydroxyphenyl)-7,8-dihydroxycoumarin is a newly synthesized coumarin derivative with a potent antioxidant effect. The aim of the present study is to investigate the safety of this compound, determining the in vitro cytotoxic and genotoxic in human peripheral blood mononuclear cells (PBMC) and in HepG2/C3A cells. Cell viability has been investigated by the trypan blue staining test and MTT assay and the genotoxicity by the comet assay and micronucleus test, using concentrations between 0.01 and 10 µg/ml. The compound proved to be noncytotoxic in both cell lines, at all tested concentrations, protecting the cells from the DNA damage. In addition, this molecule does not show clastogenic/aneugenic effects when performing the micronucleus test with cytokinesis blockade. Based on the obtained data, and the conditions of the experiments, we can conclude that the 3-(3,4-dihydroxyphenyl)-7,8-dihydroxycoumarin is a safe molecule up to a concentration of 10 µg/ml, which encourages further studies aiming to explore its potential as a drug candidate.


Assuntos
Leucócitos Mononucleares , Leucócitos , Humanos , Ensaio Cometa , Umbeliferonas/toxicidade , Dano ao DNA , Testes para Micronúcleos , Mutagênicos
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