[Calcium intake among school children in Badajoz]. / Ingesta de calcio en escolares de Badajoz.

BACKGROUND: The intake of calcium has been studied in the diet of adolescents from a school in Badajoz (Spain) determining their BMI and their living habits (sport, consumption of tobacco and alcohol). METHODOLOGY: By means of an aleatory sampling, a sample n was selected = 207 (49.76% males and 50.24 females) with an average age of 14.14 +/- 2.08 years. They were weighed and measured to calculate their BMI. A questionnaire was carried out on consumption of products rich in calcium: milk and dairy produce, vegetables, fruit, chied fruits. The statistical analysis was carried out by means of Student t and ANOVA. RESULTS: The results was BMI < 25 (thin or standard) in 86.47%; BMI 25-30 (overweight) in 10.63% and BMI > 30 (overweight) 2.9%. The consumption of daily calcium was of 1304 +/- 702 g/ppd, higher (p < 0.001) in boys (10.45 +/- 5.51) than in girls (7.82 +/- 3.84). There is not correlation between BMI and calcium consumption. The boys observe the NIH recommendations. But girls usually don't. Milk products provide 87% of the consumed calcium. The boys who practice some sports consume 9.88 +/- 5.23 g/pps, the other ones 7.09 +/- 2.95 g/pps. CONCLUSIONS: 1. The weekly intake of calcium by surveyed students is 9.13 g/pps, smaller in girls than in boys. 2. Milk products provide 87% of the consumed calcium. 3. A 10.63% of them is overweight and a 2.9% is very overweight. 4. A 10.63% usually smokes and a 20.29% consumes alcohol. 5. A 26.27% doesn't practice any sports and consumes less calcium (p < 0.001) than the recommended quantity.

Antimetastatic effects of liposome entrapped indomethacin.

The "in vivo"administration of sized liposomes encapsulating indomethacin to mice bearing 3LL tumor, significantly reduced the incidence and/or number of superficial lung metastases. Also liposomes encapsulating indomethacin had significant inhibitory effects on the experimentally induced lung metastases. We conclude: i) indomethacin encapsulated in liposomes is more efficient than the free drug in mediating the antimetastatic effects and ii) liposomes are an valuable vehicle in evading the side metastatic effects of this drug during indomethacin treatment of tumor bearing mice.

Correlation between secretion pattern and metastatic potential from drug-treated 3LL tumor cell line.

In the present study, we described the effects of three different drugs, A23187 calcium ionophore (A23187), indomethacin (IND) and phorbol myristate acetate (PMA), on the secretion pattern and the metastatic potential in the low metastatic 3LL tumor cell line. The results evidence that a low molecular weight protein fraction is always secreted in an higher amount in drug treated cells than in untreated cells independently of the drug used. In addition to their effects on secretion pattern, the three drugs assayed enhance noticeably the metastatic rate in vivo of the 3LL cells. The data confirm our earlier observations showing that our high performance gel permeation chromatography (HPGPC) method is suitable for discriminating even between cells with a different degree (low and high) of metastatic potential by its chromatographic secretion pattern and that alterations in the secretion pattern from treated cells are related with changes in the degree of metastatic potential of these cells.

HPLC analysis of supernatant soluble factors from tumor cell variants with defined metastatic status.

In the present study, we report a simple and sensitive procedure to study supernatant soluble factors of short time cultured cells by high performance gel permeation chromatography (HPGPC). This procedure enables a direct analysis with no preparatory procedures prior to application in the chromatographic column and eliminates tedious and often low reproducible techniques, required during conventional assay of culture medium. In addition, we show that HPGPC analysis is suitable for discriminating between two variants of a rhabdomyosarcoma with different metastatic potential, while two distinct cell lines (3LL carcinoma and B16F10 melanoma), which do not differ in their metastatic potential, do not present noticeable qualitative differences in their chromatographic secretion pattern.