Effect of obesity on tooth movement using an orthodontic device and changes in inflammatory cytokines, periodontal tissues, and orofacial pain.

OBJECTIVE: This study aimed to investigate the effect of obesity prospectively on tooth movement via an orthodontic device and changes in inflammatory cytokines, periodontal tissues, and orofacial pain. SUBJECTS AND METHODS: Prospective design in which data was gathered at baseline T0, 1 hour, 24 hours, and 1 week after the application of fixed orthodontic appliances. The total sample size was 60 participants aged between 12 to 18 years and divided into 2 groups based on the inclusion and exclusion criteria. Anthropometrical estimation was made using a bioimpedance meter. A clinical assessment was performed before the application of fixed appliance bonding (T0) one hour after bonding (T1), after 24 hours (T2), and finally after one week (T3). At T0 little irregularity index was assessed, gingival crevicular fluid (GCF) was collected and periodontal examination including probing depth (PD), gingival bleeding (GB), and the presence or absence of calculus were measured. Orofacial pain was assessed at three levels: 1 hours, 24 hours, and 1 week after application of fixed orthodontics using a visual analog scale. For inter-group comparison, Mann-Whitney and t-tests were used and for interphase, comparison cluster analysis was performed. The level of significance was p<0.05. RESULTS: The participants in obese groups were significantly higher in terms of weight, BMI, WHR, FM, and BF than in the non-obese group (p<0.05). Obese participants had significantly more PD 4-5 mm (0.64±0.23 mm) and significantly higher BoP than non-obese participants (0.13±0.10 mm). Little's irregularity index at T0 and T3 showed no significant difference among obese and non-obese participants. The inflammatory cytokines level of IL-ß was higher in the obese group compared to non-obese groups. CONCLUSIONS: The intensity of orofacial pain was higher in obese participants after 24 hours along with high levels of IL-ß pro-inflammatory cytokines before and during orthodontic treatment. No difference was noted in tooth movement in both obese and non-obese during orthodontic treatment in the first week.

Natural and synthetic photosensitizers activated by photodynamic therapy on enamel reconditioning rebonded to metallic brackets: an in vitro study.

OBJECTIVE: To evaluate the bond efficacy and failure rates of rebonded metallic brackets after enamel reconditioning with chemical 37% phosphoric acid (PA) and natural and synthetic photosensitizers activated by PDT. MATERIALS AND METHODS: A total of 50 non-cavitated, and cautiously extracted human premolars were congregated after sample size calculation. The enamel exterior was etched, washed, dried for adhesive application, and cured. Metallic brackets were then oriented and adapted to enamel surface using composite. Later, brackets were debonded from the surface via a Weingart plier. Enamel was finished for ensuing surface reconditioning. Ultimately, specimens were randomly distributed into five groups (n=10). Enamel surface before rebonding was reconditioned with curcumin photosensitizer (CP), riboflavin photosensitizer (RP), rose bengal photosensitizer (RBP), methylene blue photosensitizer (MBP), and 37% PA (control) respectively. After following reconditioning protocol, brackets were rebonded to the enamel exterior employing a composite adhesive system. Then, specimens were subjected to the universal testing machine for analyzing shear bond strength (SBS), and bond failures were predicted using an ARI index. One-way ANOVA and Tukey multiple comparison tests were used for statistical analysis at a variance value of p < 0.05. RESULTS: Enamel reconditioned with 37% PA demonstrated the highest SBS for bracket rebonding, and the lowest SBS was presented by CP actuated by PDT. Enamel reconditioned with RP and RBP corroborated the analogous SBS outcome to 37% PA. Likewise, enamel surface treatment with MBP revealed a statistically significant result to CP for metallic bracket rebonding. The most prevalent failure scores anticipated among groups were 0 and 1 indicating an adhesive failure with the exemption of group 5 (control) that encountered more score 2 cohesive failure on debonding metallic brackets from enamel exterior. CONCLUSIONS: Rose bengal and riboflavin photosensitizers activated by photodynamic therapy with low ARI scores have the potential to be used as viable enamel reconditioning alternatives to 37% phosphoric acid for rebonding metallic brackets.