A comparative study of photoplethysmogram and piezoelectric plethysmogram signals.

The Photoplethysmogram (PPG) signal is one of the most important vital signals in biomedical applications. The non-invasive property and the convenience in the acquisition of both PPG and Piezoelectric Plethysmogram (PZPG) signals are considered as powerful and accurate tools for biomedical diagnosing applications, such as oxygen saturation in blood, blood flow, and blood pressure measurements. In this paper, a number of features for PPG and PZPG signals (ex. first derivative, second derivative, area under the curve and the ratio of systolic area to the diastolic area) are acquired and compared. The results show that both systems are able to extract the pulse rate (PR) and pulse rate variability (PRV), accurately with an estimation error of less than 10%. The averaged standard deviation of the ratio of the systolic area to the diastolic area for the first derivative of PPG and PZPG signals was small with less than 0.49 and 0.69 for the PPG and PZPG, respectively. Statistical analysis techniques (such as cross-correlation, P-value test, and Bland Altman method) are performed to address the relation between the PPG and PZPG signals. All of these methods showed a strong relationship between the features of the two signals (i.e. PPG and PZPG). The correlation value is found to be 0.954 with a p-value of < 0.05. This opens possibilities for combining both the PPG and PZPG systems to extract more features that can be used in diagnosing cardiovascular diseases. Such a system can provide a possibility to reduce the number of devices connected to patients (especially in emergencies) by means of measuring simultaneously both signals (PZPG and PPG).

Molecular characterization and bio-functional property determination using SDS-PAGE and RP-HPLC of protein fractions from two Nigella species.

This study aimed to investigate the molecular and bio-functional properties of protein fractions from Nigella damascena and Nigella arvensis, including the albumin, globulin, glutein-1, glutein-2 and prolamin fractions. Protein subunits were not observed in globulin and prolamin fractions. No peaks appeared in RP-HPLC chromatograms of globulin for either species. Two predominant peaks were observed in the RP-HPLC profiles of all protein fractions. Proteins separated by RP-HPLC have potential inhibitory and antioxidant activities in all fractions. Optimum ACE-inhibitory and antioxidant activities of proteins separated by RP-HPLC were observed in glutein-2 and albumin, respectively, for both species. For pepsin and combined pepsin-trypsin hydrolyses, the highest degree of hydrolysis (DH) was obtained in glutein-2 fraction of Nigella arvensis. Highest ACE-inhibitory activity of hydrolyzed protein fractions was found at 4h via pepsin hydrolysis in globulin fraction of Nigella damascena. Highest antioxidant activities of hydrolyzed protein fractions were found in glutelin-2 for both species.