RESUMO
The utility of green silver nanoparticles (AgNPs) in veterinary medicine is steadily increasing as they have many therapeutic applications against pathogens and arthropods of livestock. In this study, green AgNPs using neem (N-AgNPs), 2,3-dehydrosalanol (2,3-DHS-AgNPs) and quercetin dihydrate (QDH-AgNPs) were synthesised and characterised. Synthesised compounds were characterised by UV-Vis spectroscopy and the peak absorbance was recorded at 370 nm for neem extract. For N-AgNPs, 2,3-DHS-AgNPs and QDH-AgNPs, the maximum absorbance peaks were at 430, 230 and 220 nm, respectively. The FTIR analysis confirmed the synthesis of green AgNPs. The XRD pattern of N-AgNPs showed the peaks corresponding to whole spectra of 2 θ values ranging from 10-80. The relatively higher intensity of (111, 222) planes in face centred cubic crystalline structure supports the formation of synthesised AgNPs. In DLS analysis, the hydrodynamic diameter of neem leaf extract was found to be 259.8 nm, followed by 5.3, 6.7 and 261.8 nm for 2,3-DHS-AgNPs, N-AgNPs and QDH-AgNPs, respectively. Based on the transmission electron microscopy and scanning electron microscopy image analyses, confirmed the formation of N-AgNPs, 2,3-DHS-AgNPs and QDH-AgNPs. These eco-friendly phyto-AgNPs may be of use as an effective alternative to chemical control methods against the arthropods of livestock.
Assuntos
Acaricidas/administração & dosagem , Azadirachta/química , Benzotiadiazinas/administração & dosagem , Nanopartículas Metálicas/administração & dosagem , Quercetina/administração & dosagem , Prata/administração & dosagem , Acaricidas/química , Benzotiadiazinas/química , Química Verde/métodos , Teste de Materiais , Nanopartículas Metálicas/química , Nanopartículas Metálicas/ultraestrutura , Tamanho da Partícula , Extratos Vegetais/administração & dosagem , Extratos Vegetais/química , Folhas de Planta/química , Quercetina/química , Resultado do TratamentoRESUMO
Nanobiotechnology is an emerging biological branch of nanotechnology. Application of nanoparticles with specific size and shape in biology has already shown unforeseen and interesting results. A study was conducted to evaluate the therapeutic potential of phytogenically derived aloin mediated nanosilver particles (AAgNPs), prepared by reduction of silver nitrate with aloin, in Staphylococcus aureus induced murine mastitis. A total of 40 female mice were divided into five groups of eight animals each. Group I served as lactating control, groups II-V were inoculated with 20âµl of 24â h broth culture of S. aureus containing 4.0 × 105â cfu/quarter under ketamine anaesthesia. After 6â h post inoculation, groups III and IV received 20âµl of aloin nanosilver (AAgNPs) through intramammary and intraperitoneal routes, respectively. Group V received antibiotic cefepime at 1â mg/kg body weight through the intra-peritoneal route. After 18â h post-treatment, serum C reactive protein, weights of mammary glands, mammary gland bacterial load, thiobarbituric acid reactive substances content, reduced glutathione content, superoxide dismutase activity and catalase activity and histopathology were determined. The compound showed a minimum inhibitory concentration of 21.8â ng/ml against S. aureus. Significant reduction (98%) in poly-morpho nuclear cell infiltration was observed with AAgNPs than antibiotic (50%).