Effects of Repeated in-vitro Exposure to Saudi Honey on Bacterial Resistance to Antibiotics and Biofilm Formation.

Introduction: Although Sumra and Sidr Saudi honey is widely used in traditional medicine due to its potent activity, it is unknown whether its prolonged usage has impact upon bacterial virulence or leading to reduced antibiotic sensitivity. Thus, the study aims to investigate the effect of prolonged (repeated) in-vitro exposure to Saudi honey on the antibiotic susceptibility profiles and biofilm formation of pathogenic bacteria. Methods: Several bacteria, including Staphylococcus aureus, Escherichia coli, and Acinetobacter baumannii, were in-vitro exposed ten times [passaged (P10)]to Sumra and Sider honey individually to introduce adapted bacteria (P10). Antibiotic susceptibility profiles of untreated (P0) and adapted (P10) bacteria were assessed using disc diffusion and microdilution assays. The tendency regarding biofilm formation following in-vitro exposure to honey (P10) was assessed using the Crystal violet staining method. Results: Adapted (P10) bacteria to both Sumra and Sidr honey showed an increased sensitivity to gentamicin, ceftazidime, ampicillin, amoxycillin/clavulanic acid, and ceftriaxone, when compared with the parent strains (P0). In addition, A. baumannii (P10) that was adapted to Sidr honey displayed a 4-fold increase in the minimal inhibitory concentration of the same honey following in-vitro exposure. 3-fold reduction in the tendency toward biofilm formation was observed for the Sumra-adapted (P10) methicillin resistant S. aureus strain, although there was a lower rate of reduction (1.5-fold) in biofilm formation by both the Sumra- and Sidr-adapted A. baumannii (P10) strains. Conclusion: The data highlight the positive impact of prolonged in-vitro exposure to Saudi honey (Sumra and Sider) for wound-associated bacteria since they displayed a significant increase in their sensitivity profiles to the tested antibiotic and a reduction in their ability to form biofilm. The increased bacterial sensitivity to antibiotics and a limited tendency toward biofilm formation would suggest the great potential therapeutic use of this Saudi honey (Sumra and Sidr) to treat wound infections.

Awareness About Total Knee Arthroplasty Among Hail Population.

BACKGROUND: Knee joint osteoarthritis is the most common among older adults. The knee joint needs to be treated surgically. The prevalence of this disorder is believed to be higher than 40% among people over 60. OBJECTIVE: To study public awareness about total knee replacement among the Hail population and to explore the relationship between the level of awareness and different socio-demographic factors. METHODS: A cross-sectional study was carried out in Hail, Saudi Arabia, using an online self-administered questionnaire created via Google Forms. Collected data were coded, entered, and analyzed using the Statistical Package for Social Sciences (SPSS; Version 23). RESULTS: A total of 431 participants enrolled in the study, of which 242 (56.15%) were males and 189 (43.9%) were females. Out of a total of 34 points, the average awareness score was 15 ± 6 (range: 5-33), with a mean percentage of awareness of 44.1%. The most reported causes of total knee replacement were overweight or obesity (82.6%), aging (61%), and rheumatoid arthritis (45.2%). The most known symptoms were pain (85.6%) and clicking sounds during movements (64.7%). Regarding the knowledge about the treatment, both physiotherapy and weight reduction were mentioned by 290 (67.3%), exercise by 244 (56.6%), and surgical intervention by 177 (41.1%) participants. The purpose of knee replacement surgery was to relieve pain according to 357 (82.8%) participants, improve walking quality according to 257 (59.6%) participants, and be able to do prayers (while kneeling) according to 215 (49.9%) participants. The most reported reasons that might prevent the participants from undergoing knee replacement surgery were the pain after surgery (59.4%), unavailability of surgeons (35.5%), and general complications from anesthesia (37.4%). Moreover, 188 (43.6%) participants thought that knee osteoarthritis is diagnosed using magnetic resonance imaging (MRI). Educational level was found to be associated with awareness score (p = .012). Conversely, gender, nationality, residence, and history of chronic disease were not found to be associated with awareness about total knee replacement (p = .548, .357, .734, and .639, respectively). CONCLUSION: An average level of awareness and knowledge regarding total knee replacement was observed. Educational level was found to be significantly associated with this awareness.

Trends in Intelligent and AI-Based Software Engineering Processes: A Deep Learning-Based Software Process Model Recommendation Method.

In recent years, numerous studies have successfully implemented machine learning strategies in a wide range of application areas. Therefore, several different deep learning models exist, each one tailored to a certain software task. Using deep learning models provides numerous advantages for the software development industry. Testing and maintaining software is a critical concern today. Software engineers have many responsibilities while developing a software system, including coding, testing, and delivering the software to users via the cloud. From this list, it is easy to see that each task calls for extensive organization and preparation, as well as access to a variety of resources. A developer may consult other code repositories, websites with related programming content, and even colleagues for information before attempting to build and test a solution to the problem at hand. In this investigation, we aim to identify the factors that led to developing the recommender. This system analyzes the recommender's performance and provides suggestions for improving the software based on users' opinions.