RESUMO
OBJECTIVE: This in vitro study evaluated cytotoxicity and cell migration effects of zoledronic acid and irradiation upon oral squamous cell carcinoma. MATERIALS AND METHODS: Zoledronic acid was administrated at doses of 10, 25, 50, and 100 µM to PE/CA-PJ15 oral squamous cell carcinoma cultures, irradiated with different doses (0, 5, 15, and 30 Gy), followed by evaluation of the effects on cell viability. Cell migration capacity was studied after 24- and 72-h incubation. RESULTS: At 24 h, the 100 µM concentration of zoledronic acid combined with 15 Gy irradiation caused the greatest decrease in cell viability. At 72 h, statistically significant decreases in cell viability were found with all concentrations of zoledronic acid with or without irradiation: 0 Gy (P < 0.001), 5 Gy (P < 0.001), 15 Gy (P < 0.001), and 30 Gy (P < 0.001). 50 µM and 100 µM doses of zoledronic acid combined with 5 Gy irradiation yielded the greatest decrease in cell migration capacity. CONCLUSIONS: Zoledronic acid increases cytotoxic activity in the PE/CA-PJ15 cell line and reduces cell migration capacity. These findings suggest that combination therapy using biphosphates and radiation may offer a promising therapy.
Assuntos
Antineoplásicos/uso terapêutico , Carcinoma de Células Escamosas/tratamento farmacológico , Difosfonatos/uso terapêutico , Imidazóis/uso terapêutico , Neoplasias Bucais/tratamento farmacológico , Antineoplásicos/administração & dosagem , Carcinoma de Células Escamosas/patologia , Carcinoma de Células Escamosas/radioterapia , Técnicas de Cultura de Células , Linhagem Celular Tumoral , Movimento Celular/efeitos dos fármacos , Movimento Celular/efeitos da radiação , Sobrevivência Celular/efeitos dos fármacos , Sobrevivência Celular/efeitos da radiação , Difosfonatos/administração & dosagem , Relação Dose-Resposta a Droga , Relação Dose-Resposta à Radiação , Avaliação Pré-Clínica de Medicamentos , Humanos , Imidazóis/administração & dosagem , Neoplasias Bucais/patologia , Neoplasias Bucais/radioterapia , Dosagem Radioterapêutica , Fatores de Tempo , Ácido ZoledrônicoRESUMO
OBJECTIVE: The aim of this study was to investigate salivary markers related to burning mouth syndrome (BMS). STUDY DESIGN: A cross-sectional prospective controlled clinical study of 58 patients (28 BMS and 30 control). The presence of mineral and trace metals in unstimulated whole saliva was analyzed in BMS patients and in control subjects by means of ICP-OES. The parameters analyzed were as follows: oral health-related quality of life (OHIP-14) and the Hospital Anxiety Depression (HAD) scale. A visual analogue scale (VAS) was used to measure the intensity of burning sensation (pain). RESULTS: The mean pain value for patients with BMS was 7.8 ± 2.4. OHIP-14 oral health-related quality of life among BMS patients was found to be affected: total BMS OHIP-14 (16.98 ± 12.29) vs. control (8.53 ± 10.5) with significant difference (P = 0.015). Concentrations of the different elements were slightly higher among BMS patients but without statistically significant differences for any of the elements analyzed (Na, K, Ca, Mg, Fe, Cu, Mn, Zn, B, P, S, Al, Pb, Cd, Cr, Ni, As, Be, Bi, Co, Li, Mo, Sb, Se, Sr, Ti, Tl, V). CONCLUSIONS: This study failed to identify abnormal levels of minerals or trace elements in saliva of patients with BMS.
