Digital Form for Assessing Dentists' Knowledge about Oral Care of People Living with HIV.

Oral lesions are usually the first sign of HIV infection. The present study aimed to determine the level of the knowledge of dentists on the dental care needs of People Living with HIV (PLWH). This cross-sectional study was conducted between February and May 2021, in the Brazilian state of Pará, during which a total of 51 dentists received an anonymous digital form (Google® Forms Platform) composed of four blocks of discursive, dichotomous, and multiple-choice questions. The questions referred to various aspects of the dental care needs of PLWH, together with data on the professional activities of the dentists. After signing the term of informed consent, the dentists were divided into six subgroups according to the time (in years) since completing their bachelor's degree in dentistry. The data were presented as descriptive statistics and percentages, and then analyzed using the Kappa test. Most (70.6%; 36 of 51) of the dentists were female, the mean age of the dentists was 32.5 years, and a majority (80.2%) were based in the city of Belem; the mean time since graduation was 8.5 years, with 22 (43.1%) having more than 5 years of professional experience, and 31 (60.8%) having graduated from a private dental college. Just over half (51%) of the 51 dentists had completed graduate courses, and the most common dental specialty was orthodontics (19.6%). Most (74.5%) of the dentists work in the private sector, 38 (74.5%) claimed to have already provided oral care to PLWH, and 43 (84.3%) had access to specialist content on the oral care needs of PLWH. In terms of the knowledge of the dentists with regard to the oral care needs of PLWH, four of the ten diagnostic questions obtained more inadequate answers than expected, whereas the final two questions (11-45.1% and 12-31.4%) demonstrated that many of the dentists adopt unnecessary modifications in their oral care protocol for PLWH, due to a fear of contamination. Overall, our results demonstrate a frequent lack of knowledge, especially with regard to the oral healthcare needs of PLWH, which may account for many of the stigmas that persist in the dental care of this vulnerable group.

Disparities in Stroke Patient-Reported Outcomes Measurement Between Healthcare Systems in Brazil.

Introduction: Acute stroke interventions, such as stroke units and reperfusion therapy, have the potential to improve outcomes. However, there are many disparities in patient characteristics and access to the best stroke care. Thus, we aim to compare patient-reported outcome measures (PROMs) after stroke in two stroke centers representing the public and private healthcare systems in Brazil. Methods: PROMs through the International Consortium for Health Outcomes Measures (ICHOM) were assessed at 90 days after the stroke to compare two Brazilian hospitals in southern Brazil: a public university and a private stroke center, both with stroke protocols and stroke units. Results: When compared with the private setting (n = 165), patients from the public hospital (n = 175) were younger, had poorer control of risk factors, had more frequent previous strokes, and arrived with more severe strokes. Both hospitals had a similar percentage of IV thrombolysis treatment. Only 5 patients received mechanical thrombectomy (MT), all in the private hospital. Public hospital patients presented significantly worse outcomes at 3 months, including worse quality of life and functional dependence (60 vs. 48%, p = 0.03). Poor outcome, as measured by the mRS score, was significantly associated with older age, higher NIHSS score, and the presence of heart failure. However, the public practice was a strong predictor of any self-reported disability. Conclusion: Patients assisted at a good quality public stroke center with the same protocol used in the private hospital presented worse disability as measured by mRS and patient-reported outcome measures, with greater inability to communicate, dress, toilet, feed, and walk.

Simultaneous occurrence of Epstein-Barr virus (EBV) in periodontal pockets and in oral squamous cell carcinoma: a cross-sectional study.

OBJECTIVES: This study aimed to investigate the detection of Epstein-Barr virus (EBV) in oral squamous cell carcinoma (OSCC) and to verify the concordance of EBV-DNA frequency in subgingival sites and in the OSCC. METHODS: A cross-sectional study with 30 OSCC patients, aged from 44 to 88 years old, was conducted. Samples were collected in subgingival sites and at the OSCC, then submitted to DNA isolation, qPCR, and genotyping. Descriptive statistic was performed to report the frequency of EBV-DNA in all samples, and McNemar test was applied to verify the concordance among the EBV-DNA frequency in both sites. RESULTS: The individuals presented 62 years old in average, and the majority were male (66.6%). EBV-DNA was detected in 56.7% OSCC lesions. Among the subgroup of 19 dentate individuals, high concordance (73.7%) in both EBV-DNA detection and the absence in subgingival sites and OSCC was observed, and it was statistically significant (p < 0.05). CONCLUSIONS: We report the notable occurrence of EBV-DNA in OSCC; also, the presence of EBV in periodontal sites may contribute to find it in OSCC, although the possible contribution of EBV in the OSCC remains to be investigated. CLINICAL RELEVANCE: The identification of this easily accessible site of EBV latent infection may help to improve the patient's quality of life by maintenance of oral/periodontal health condition and preventing further possible disorders related to the virus, and also encourages new approaches for investigating EBV, periodontitis, and OSCC.

Incorporação de tecnologias digitais nas estrategias de apoio à Renast-BA durante a pandemia da Covid-19 / Incorporation of digital Technologies as a strategy for suporte to Renast-BA during Covid-19 pandemic / Incorporación de tecnologias digitales en estrategias de apoyo à Renast-BA durante la pandemia del Covid-19

As principais medidas de prevenção à pandemia da Covid-19, de distanciamento e isolamento social, implicaram na necessidade de reorganização dos processos de trabalho das equipes de saúde. A substituição das atividades presenciais pela utilização de ferramentas digitais de informação e comunicação possibilitou a reorganização de práticas e estabelecimento de vínculos, antes distantes nos territórios, garantindo maior participação, inserção e atuação dos profissionais de saúde no âmbito da Vigilância da Saúde do Trabalhador, no enfrentamento da Covid-19 no estado da Bahia. Este trabalho objetiva descrever a incorporação de tecnologias digitais no desenvolvimento do apoio institucional e técnico e das atividades de educação permanente pelo Cesat/Divast para técnicos da Rede Estadual de Atenção Integral à Saúde do Trabalhador da Bahia no contexto da pandemia da Covid-19. Foram realizadas webcapacitações semanais, webreuniões de apoio institucional e matricial, webpalestras e webtreinamentos, por meio das plataformas Telessaúde e Teams, com discussão de orientações e notas técnicas, troca de experiências, propiciando ampliar a qualificação técnica das equipes de saúde do trabalhador na pandemia. Os principais resultados foram: ampliação das ações de saúde do trabalhador no estado, integração das equipes, mudanças de práticas, aprendizagem e troca de experiências no enfrentamento da pandemia, em contexto de restrição de encontros presenciais e de necessidade de prevenção da exposição de trabalhadores. Destaca-se a importância e necessidade de ampliar a capacidade, os investimentos e o acesso aos recursos tecnológicos para toda a Renast-BA, bem como manter e integrar essas estratégias às modalidades presenciais, inclusive no período pós-pandemia.

The main measures to prevent Covid-19 pandemic, such as social distance and isolation, implied the need to reorganize the work processes of health teams. The substitution of face-to-face activities with the use of digital information and communication tools enabled the reorganization of practices and the establishment of links, previously distant in the territories, ensuring greater participation, insertion and performance of health professionals within the scope of Worker's Health Surveillance, mainly when facing Covid-19 in the state of Bahia. This study aims at describing the incorporation of digital technologies in the development of support and permanent education activities by the Worker's Health Reference State Center, Worker's Health Surveillance and Care Directorate for health professionals in the Worker's Health Comprehensive Attention Network of Bahia in the context of the Covid-19 pandemic. Moreover, it was possible to expand the technical qualification of health teams on various topics of occupational health. The greatest impact achieved was the decentralization of occupational health actions in the state, transformations in practices and the opportunity for learning and exchange, with excellent professional contributions, in a period when traveling to the territories and face-to-face meetings were restricted to avoid agglomerations and exposure of workers.

Las principales medidas preventivas de la pandemia del Covid-19, la distancia y el aislamiento sociales, implicaron en la necesidad de reorganización de los procesos de trabajo del personal sanitario. La sustitución de las actividades presenciales por el uso de herramientas de información y comunicación digitales permitió la reorganización de prácticas y el establecimiento de vínculos, anteriormente distantes en los territorios, asegurando una mayor participación, inserción y desempeño de los profesionales de la salud en el ámbito de la vigilancia en salud ocupacional frente al Covid-19 en el estado de Bahía. Este trabajo tiene como objetivo describir la incorporación de tecnologías digitales en el desarrollo de actividades de apoyo y educación permanente por parte del Centro Estadual de Referencia en Salud Ocupacional/Dirección de Vigilancia y Atención a la Salud Ocupacional, destinadas a técnicos de la Red Estadual de Atención Integral a la Salud Ocupacional de Bahía en el contexto de la pandemia del Covid-19. Se realizaron en línea capacitaciones semanales, encuentros de apoyo institucional y matricial, conferencias y entrenamientos utilizando las plataformas Telessáude y Teams, en las cuales hubo discusión de los lineamientos y notas técnicas, intercambio de experiencias, lo que permitió ampliar la cualificación técnica del personal sanitario en la pandemia. Los principales resultados fueron: ampliación de la actuación sanitaria del trabajador en el estado, integración entre el personal, transformaciones en las prácticas, aprendizaje e intercambio de experiencias en el enfrentamiento de la pandemia, en un período de restricción de las reuniones presenciales y la necesidad de prevenir la exposición de los trabajadores. Cabe señalar la importancia y necesidad de ampliación de la capacidad, las inversiones y el acceso a las herramientas tecnológicas para toda Renast-BA, así como de mantenimiento e integración de esas estrategias a las modalidades presenciales, incluso en el período pospandémico.

Epstein-Barr virus (EBV) in periodontal sites of human immunodeficiency virus (HIV)-positive individuals in North Brazil: a cross-sectional study.

OBJECTIVE: This study aimed to investigate the Epstein-Barr virus (EBV) prevalence and viral load in subgingival sites of human immunodeficiency virus type 1 (HIV-1) positive (HIV+) individuals, correlating subgingival EBV load to the clinical periodontal condition, HIV systemic load, EBV systemic load, and use of antiretroviral therapy (ART). METHOD AND MATERIALS: Ninety individuals were recruited and divided into three categories: those without periodontal disease (G1), with gingivitis (G2), and with periodontitis (G3). Subgingival biofilm and blood samples were analyzed by quantitative polymerase chain reactions (qPCR). A questionnaire was administered to collect general information about patients, and data regarding HIV and use of ART were accessed from their medical records. RESULTS: EBV was detected in 85.6% of the samples. Comparing subgingival and systemic load of EBV in G1, G2, and G3, there was a statistical difference only in G3 (3.93 log10 copies/mL and 5.47 log10 copies/mL, respectively; P = .014), where EBV load was higher in periodontal pockets than in the blood. All groups had high EBV loads in subgingival sites (> 2,000 copies/mL). A positive linear correlation between systemic HIV load and EBV subgingival load was found in G1 and G2 (r = 0.647; P < .001), but not in G3. Only G1 individuals using ART had lower subgingival EBV loads than those not using it (5.03 log10 copies/mL, and 7.14 log10 copies/mL, respectively; P = .0348). CONCLUSIONS: Subgingival sites, especially the periodontal pockets, are suggested to act as a reservoir of EBV in HIV+ individuals. Therefore, the identification of latent EBV infections in this easily accessible site might help to improve quality of life in patients with HIV by maintaining oral/periodontal health. In addition it might encourage new approaches in investigating EBV-associated disorders in HIV+ patients.

Creeping attachment após enxerto gengival livre: relato de caso / Creeping attachment after autogenous free gingival grafting: case report

Introdução: As técnicas de enxerto gengival livre têm como principal objetivo a criação/aumento da faixa de mucosa ceratinizada. Quando se utiliza o tratamento químico da raiz e o correto posicionamento do enxerto no leito receptor podem acarretar em um fenômeno incomumdenominado de Creeping Attachment. Objetivo: descreverum caso de recobrimento radicular apósum enxerto gengival livre e Creeping Attachment. Relato do caso: Paciente sexo masculino, 28 anos de idade, não fumante e sem alterações sistêmicas apresentouse a faculdade de odontologia Universidade Federal de Santa Catarinacom a queixa principal de comprometimento estético e hipersensibilidade dentinária na região anterior-inferior. No exame clínico verificou-se uma recessão gengival unitária no dente 41, classificada como classe I de Miller. Logo indicou-se a cirurgia de enxerto gengival livre, obtido da região de palato duro, visandouma melhora estética efuncional, bem como a remissão da hipersensibilidade. Conclusão: Diante dos resultados clínicos podemos concluir que o enxerto gengival livre é uma técnica previsível e de fácil realização para o aumentoda faixa de mucosa queratinizada para posterior recobrimento radicular, contudo apesar de incomum o fenômeno Creeping Attachment deve ser esperado como alternativa a etapade recobrimento radicular. (AU)

Introduction: Free gingival graft techniques have as main objective the creation / increase of the keratinized mucosa band. When using the chemical treatment of the root surfaceand the correct positioning of the graft in the recipiente bed can lead to anuncommon phenomenon calledCreeping Attachment. Objective: This scientific paper describes a clinical case of root coverage after a free gingival graft and Creeping Attachment. Case Report: A 28-year-old male patient, non-smoker, was assisted in the Federal University of Santa Catarina by students and teachers of dentistry presented the main complaint the aesthetic impairment and dentin hypersensitivity in the anterior region of the jaw. In the clinical examination was observed an unitary gingival recession in tooth 41, classified as Miller class I. The gingival graft surgery, obtained from the hard palate region, was then indicated to improve the esthetic and functional condition, as well as to promove the end of symptoms of hypersensitivity. Conclusion: Inview of the clinical results we can conclude that the free gingival graft is a predictable and easy to perform technique to increase the line of keratinized mucosa for the subsequent root coverage. However, although the Creeping Attachment phenomenon is uncommon, it should be expected as an alternative to the root coverage (AU)

Odontologia e saúde mental: experiência do PET Saúde no Centro de Atenção Psicossocial Álcool e Drogas / Odontology and mental health: experience of PET Saúde in the Center for Psychosocial Care Alcohol and Drugs

A fim de discutir a importância de experiências multiprofissionais e o papel de inovação do PET - Saúde na formação acadêmica, este artigo relata a experiência do PET - Odontologia da UFPA junto à equipe multiprofissional de um CAPS AD localizado no município de Belém/PA, além de avaliar as condições de saúde bucal dos dependentes químicos. A experiência mostrou a integração entre os diferentes profissionais e motivou o desenvolvimento de habilidades dos discentes para o planejamento de ações integradas e criativas de promoção de saúde, através de metodologias ativas, apontando novos rumos de atenção à saúde, centrado na lógica da realidade da comunidade e não somente no ambiente clínico. As atividades realizadas pela equipe de Odontologia foram integradas às oficinas e grupos desenvolvidos na unidade, como oficinas culinárias e de confecção e o cuidado de hortas, que propiciaram momentos de educação em saúde bucal. Foram realizadas dinâmicas e rodas de conversas, sendo os usuários agentes ativos no processo de construção compartilhada de conhecimento. Ocorreu o "dia D no CAPS AD", no qual os usuários participaram de atividade de escovação supervisionada e foram examinados por equipe médica e odontológica, com coleta de informações clínicas. Considera-se que a interação entre ensino, serviço e comunidade potencializada pelo PET - Saúde conduz a formação em saúde a um novo perfil, em que os profissionais se tornam mais capazes de responder às necessidades dos usuários e atuar de maneira compartilhada nos diferentes cenários (AU).

In order to discuss the importance of multiprofessional experiences and the innovation role of the PET - Saúde in academic training, this article reports on the experience PET - Odontology of UFPA with the multi-professional team of a CAPS AD located in the city of Belém / PA, besides evaluating the oral health conditions of chemical dependents. The experience showed the integration among the different professionals and motivated the development ofthe students' abilities to the planning of integrated and creative actions of health promotion, through active methodologies, pointing out new directions of health care, centered on the logic of the community reality and not only in the clinical environment. The activities carried out by the Dentistry team were integrated with the workshops and groups developed at the unit, such as cooking and cooking workshops and garden care, which provided moments of education in oral health. Was made dynamics and wheels of conversations, and the users are active agents in the process of shared knowledge construction. "D-day at CAPS AD" occurred, in which users participated in supervised brushing activity and were examined by medical and dental staff, collecting clinical information. It is considered that the interaction between teaching, service and community enhanced by PET ­ Saúde leads health education to a new profile in which professionals become better able to respond to users' needs and act in a shared way in different scenarios (AU).

Absence of cytomegalovirus in gingivitis and chronic periodontitis in HIV-1 patients in Northern Brazil.

INTRODUCTION: The influence of cytomegalovirus (CMV) on the progression of chronic periodontitis in HIV patients is poorly investigated. METHODS: ELISA was used for anti-CMV antibody IgG titer measurements and real-time polymerase chain reaction for qualitative and quantitative CMV detection. Data on the CD4 + T lymphocyte count and plasma HIV viral load were obtained from patient records. RESULTS: CMV DNA was detected in samples of subgingival biofilm in only three individuals, two of them with chronic periodontitis (4%) and one with gingivitis (3.3%). CONCLUSIONS: The prevalence of CMV is very low both in HIV-1 patients with gingivitis and chronic periodontitis.

Absence of cytomegalovirus in gingivitis and chronic periodontitis in HIV-1 patients in Northern Brazil

Abstract INTRODUCTION The influence of cytomegalovirus (CMV) on the progression of chronic periodontitis in HIV patients is poorly investigated. METHODS ELISA was used for anti-CMV antibody IgG titer measurements and real-time polymerase chain reaction for qualitative and quantitative CMV detection. Data on the CD4 + T lymphocyte count and plasma HIV viral load were obtained from patient records. RESULTS CMV DNA was detected in samples of subgingival biofilm in only three individuals, two of them with chronic periodontitis (4%) and one with gingivitis (3.3%). CONCLUSIONS The prevalence of CMV is very low both in HIV-1 patients with gingivitis and chronic periodontitis.

Hexapod decomposers of Serra de Santa Catarina, Paraíba, Brazil: an area with high potential for conservation of Caatinga biodiversity / Hexápodes decompositores da Serra de Santa Catarina, Paraíba, Brasil: uma área com elevado potencial para a conservação da biodiversidade da caatinga

Abstract: In detritus-based trophic systems, springtails, dung beetles, saprophagous calyptrate flies and termites consume and fragment organic matter and control populations of decomposer microorganisms, exerting a strong influence on energy and nutrient fluxes. A faunal inventory of these four groups of hexapods was performed in Serra de Santa Catarina (SSC), an area of arboreal-shrub caatinga located in the state of Paraíba, with the purpose of characterizing the assemblages of these taxa, and highlighting their unique links to the local ecosystem. Samplings were performed in May, 2014, and April, 2015, both during the rainy season. Standard sampling protocols for biodiversity inventory of the various taxa were used, including both active and passive sampling methods. In general, 114 species of hexapods were captured, with 26 species of springtails, 20 dung beetles, 30 saprophagous calyptrate flies and 38 termites, with sampling sufficiency varying from 69.5 to 96.8% of total estimated richness. Species richness of the groups are among the highest recorded for a single area of Caatinga, with some taxa being recorded for the first time for the domain. Several morphospecies had indeterminate taxonomic status, especially springtails and termites, and are quite likely new species to science. The structure of the assemblages of springtails, dung beetles, saprophagous calyptrate flies and termites recorded in SSC, suggest that this conserved area is unique within the highly impacted landscape of Caatinga, and has great potential for the conservation of biodiversity of this domain in the Northeast Region of Brazil.

Resumo: No sistema trófico baseado em detritos, os colêmbolos, besouros escarabeíneos, moscas saprófagas e térmitas atuam no consumo e fragmentação da matéria orgânica e no controle das populações de microrganismos decompositores, exercendo forte influência nos fluxos de energia e nutrientes. Um inventário faunístico desses quatro grupos de hexápodes foi realizado na Serra de Santa Catarina (SSC), uma área de Caatinga arbóreo-arbustiva localizada no Estado da Paraiba, com intuito de caracterizar as taxocenoses desses táxons, salientando as suas peculiaridades ligadas ao ecossistema local. As coletas foram realizadas em maio/2014 e abril/2015, durante o período chuvoso na região. De acordo com o táxon, foram utilizados protocolos amostrais padronizados para inventários de biodiversidade, com métodos passivos e ativos de coleta. No geral, 114 espécies de hexápodes foram capturadas, sendo 26 de colêmbolos, 20 de besouros escarabeíneos, 30 de dípteros e 38 de térmitas, com suficiência amostral variando de 69,5 a 96.8% da total estimada. A riqueza de espécies por grupo está entre as maiores para uma única área de Caatinga, com alguns táxons sendo registrados pela primeira vez para o domínio. Especialmente para os colêmbolos e térmitas, várias morfoespécies tiveram seus status taxonômicos indeterminados, havendo elevada possibilidade de serem nova para a ciência. As estruturas e funcionalidades das taxocenoses de colêmbolos, besouros escarabeíneos, moscas saprófagas e térmitas registradas na SSC, sugerem que esse ecossistema é singular, dentro de um cenário generalizado de impacto antrópico presente na Caatinga, e possui um elevado potencial para a conservação da biodiversidade desse domínio no nordeste brasileiro.

Prevalência de pólipos colorretais em pacientes submetidos à colonoscopia em Curitiba, Brasil / Colorectal polyps prevalence in patients undergoing colonoscopy in Curitiba, Brazil

A colonoscopia permite observar o interior do intestino grosso e simultaneamente caracterizar as lesões observadas, ao mesmo tempo oportunizando a remoção dos pólipos encontrados. Dessa forma, objetivou-se analisar a característica morfológica, localização e a prevalência de pólipos encontrados no intestino grosso de homens e mulheres na faixa etária entre 40 e 70 anos em uma clínica privada na cidade de Curitiba - PR. Adotou-se a pesquisa quantitativa do tipo exploratória descritiva com análise retrospectiva. A coleta dos dados se realizou por meio de revisão de 612 prontuários de clientes que realizaram o exame no período de janeiro a dezembro de 2011. Dos 612 prontuários analisados, utilizaram-se 453 que se enquadravam dentro da faixa etária escolhida. A população feminina apresentou 36% de presença de pólipos, sendo a masculina com maior índice (54%). De acordo com a idade, os sujeitos de 40 a 49 anos (n=84), destes 25% apresentaram pólipos em reto e 15% em cólon; de 50 a 59 anos (n=195) 28% com pólipos em reto e 43% em cólon; de 60 a 69 anos (n=158) 43% apresentaram pólipos em reto e 39% em cólon; acima de 70 anos (n=16) 4% apresentaram pólipos em reto e 3% em cólon. A característica morfológica foi de 3% do tipo pediculado, 3% do subpediculado e 94% séssil. Percebe-se que há influência da idade no diagnóstico, havendo a necessidade da realização de mais pesquisas que discorram a respeito da morfologia dos pólipos.

Analysis of FHS community health agents knowledge about oral health / Análise do conhecimento sobre saúde bucal de agentes comunitários de saúde da ESF

The community health agents (CHA) are considered health promoters in Brazilian communities teaching them about health promotion and disease prevention, including oral health. According to the Ministry of Health, CHAs must know about seven major oral health issues in Brazil. Thus, the objective of this study was to evaluate the oral health knowledge level of CHAs in the city of Belem, Pará State, Brazil. The study was based on a self-guided script, through a pre-prepared questionnaire containing 16 multiple- choice questions related to oral health knowledge. The survey was conducted with 94 agents from seven Family Health stations featuring oral health teams in Belem. It was concluded that community agents should be better prepared about oral care, as not all oral health issues were known by the CHAs oral health teams in Belem.

Os agentes comunitários de saúde (ACS) são considerados promotores de saúde nas comunidades brasileiras instruindo-as sobre promoção de saúde e prevenção de doenças, inclusive em saúde bucal. De acordo com o Ministério da Saúde, os ACS's devem conhecer sete principais agravos bucais no Brasil. Dessa forma, o objetivo deste trabalho foi avaliar o nível de conhecimento dos ACS's em Belém, Estado do Pará, Brasil sobre saúde oral. O estudo foi baseado em um roteiro auto gerenciado, por meio de um questionário pré-elaborado, contendo 16 questões objetivas referentes ao conhecimento sobre saúde bucal. A pesquisa foi realizada com 94 agentes em sete Unidades de Saúde da Família com equipes de saúde bucal do município de Belém. E concluiu-se, portanto, que os agentes comunitários devem ser melhor preparados acerca dos cuidados bucais, pois não são todos os agravos em saúde bucal que são conhecidos pelos ACS's das equipes de saúde bucal em Belém.

Investigação do vírus Epstein-Barr em pacientes com Periodontite Crônica / Investigation of the Epstein-Barr virus in patients with chronic periodontitis

Introdução: Nos últimos anos, um número crescente de estudos sugere a participação dos herpesvírus na doença periodontal. Objetivo: Este trabalho investiga a relação entre a presença do herpesvírus Epstein-Barr (EBV) e a infecção periodontal em pacientes com periodontite crônica. Metodologia: Foram coletadas amostras de biofilme subgengival de sítios com profundidades de sondagem de 4 a 6 mm e > 7 mm, de 28 pacientes com periodontite crônica. Como controles, foram incluídos 16 indivíduos, sistemicamente saudáveis e sem doença periodontal. Adicionalmente, parâmetros clínicos de profundidade de sondagem (PS), nível clínico de inserção (NCI) e índice de sangramento à sondagem (SS) foram registrados. Resultado: Os resultados demonstraram médias de 2,7 mm PS, 1,7 mm NCI e 0,3% dos sítios apresentaram SS. A investigação do EBV no biofilme subgengival dos grupos foi realizada por meio da reação em cadeia da polimerase com primer espécie-específico. Os resultados da análise viral indicaram ausência de EBV em todas as amostras subgengivais analisadas. Conclusão: A partir destes resultados, não foi encontrada relação entre a presença do herpesvírus Epstein-Barr e a periodontite crônica.

Introduction: In recent years, a growing number of studies have suggested the participation of the herpes virus in periodontal disease. Objective: this study investigates the relationship between the presence of the Epstein-Barr herpes virus and periodontal infection in patients with chronic periodontitis. Methodology: subgingival biofilm samples were collected of subgingival sites with probing depths of 4 mm to 6 mm, and > 7 of 28 patients with chronic periodontitis. The control group consisted of 16 healthy subjects without clinical evidence of chronic periodontitis. Additionally, clinical parameters of probing depth, attachment level and Bleeding index were recorded. Result: the results showed averages of 2 mm probing depth, 1, 7 mm attachment level and 0.3 % bleeding on probing. Investigation of the herpes virus in the subgingival biofilm of the groups was performed using polymerase chain reaction with species-specific primer. Results of viral analysis indicated the absence of EBV in all subgingival samples analyzed. Conclusion: these results found no relationship between the presence of the Epstein-Barr herpes virus and chronic periodontitis.

Influence of red wine fermentation oenological additives on inoculated strain implantation.

Pure selected cultures of Saccharomyces cerevisiae starters are regularly used in the wine industry. A survey of S. cerevisiae populations during red wine fermentations was performed in order to evaluate the influence of oenological additives on the implantation of the inoculated strain. Pilot scale fermentations (500 L) were conducted with active dry yeast (ADY) and other commercial oenological additives, namely two commercial fermentation activators and two commercial tannins. Six microsatellite markers were used to type S. cerevisiae strains. The methodology proved to be very discriminating as a great diversity of wild strains (48 genotypes) was detected. Statistical analysis confirmed a high detection of the inoculated commercial strain, and for half the samples an effective implantation of ADY (over 80 %) was achieved. At late fermentation time, ADY strain implantation in fermentations conducted with commercial additives was lower than in the control. These results question the efficacy of ADY addition in the presence of other additives, indicating that further studies are needed to improve knowledge on oenological additives' use.

A clinical report of Type III dens invaginatus: relevant aspects of a combined therapeutic approach.

Dens invaginatus is a developmental abnormality that alters dental morphology; as a result, treating this condition is a challenge for endodontic practices. This article describes how a combination of nonsurgical and surgical therapies was utilized to treat a maxillary central incisor with Type III dens invaginatus and vital pulp. The treatment plan included using computed tomography (CT) for a detailed analysis of the dental anatomy and periapical area, endodontic and surgical procedures, and a 4-year follow-up period that included periodic clinical and radiographic examinations. The follow-up examinations revealed a regression of the apical lesion and no other signs or symptoms. Based on the present case report, the authors concluded that this combination of surgical and nonsurgical approaches was effective and that CT is a valuable auxiliary tool for the study of dental anatomy.

Avaliação clínica e microbiológica de prótese total deidosos em instituições assistenciais / Assessment clinical and microbiological of complete dentures in aged from health care institution

Introdução: O Brasil possui mais de sete milhões de idosos desdentados e a prótese totaldentária ainda é a principal forma de reabilitar esses indivíduos. O objetivo deste trabalhofoi avaliar clínica e microbiologicamente próteses totais usadas por idosos de instituiçõesassistenciais. Métodos - Foram avaliados 55 idosos, acima de 60 anos de idade, portadoresde prótese total superior. Foi utilizado um questionário para coleta de informações. Exameclínico do palato foi realizado para detecção de lesões inflamatórias, seguido de quantificaçãoda placa bacteriana visível e colheita de biofilme microbiano da região de palato da prótesetotal superior. As amostras de biofilme foram processadas para cultivo de leveduras do gêneroCandida. Resultados - O tempo de uso da prótese foi superior a 10 anos, com conservaçãoinsatisfatória. A higienização prevalente foi o uso de escova dental com dentifrício, comfrequência de três vezes ao dia, mas 56,4% das próteses apresentaram higienização precária.Lesões inflamatórias estavam presentes em 72,7% dos idosos e a colonização por Candidafoi detectada em 60% das próteses. O estudo das correlações revelou significância estatísticado padrão de higienização com a colonização de levedura e também com a presença de lesãono palato, enquanto o aumento no número de colônias de espécies de Candida correlacionou--se com lesões inflamatórias no palato. Conclusão - Os idosos são portadores de prótesesantigas e deterioradas, com higienização inadequada. As lesões inflamatórias fúngicas sãoprevalentes no palato. Faltam informações adequadas e programas de atendimento ao idosopara garantir melhores condições de saúde bucal.

Introduction: There are more than seven millions of completely edentulous aged in Braziland complete dentures are still the main way of rehabilitating these individuals. The purposeof this study was to appraise clinically and microbiologically complete dentures in aged fromhealth care institutions. Methods - 55 aged have been evaluated, over 60 years old, usingupper complete dentures. Questionnaire is used for take information about patients. Palateclinical examinations were made in order to detect de inflammatory lesions, in accordancewith the quantification of the visible bacterial plaque and collection of microbial biofilmof the palate region from upper complete denture. The biofilm samples were processed forcultivation of yeasts of Candida. Results - Used time of the prosthesis was above 10 years,which presented an unpleasant state of conservation. The common form of hygienization wasthe toothbrush with dentifrice, with frequency of three times a day, but 56,4% of the prosthesispresented precarious hygienization. The inflammatory lesions were present in 72,7% ofthe aged and the colonization by Candida was detected in 60% of the prosthesis. The studyof the correlations revealed statistical significance of the hygienization pattern along withthe colonization of yeasts and also with the presence of inflammatory lesions in the palate.Conclusion - The aged are users of old and damaged prosthesis, with inadequate hygienization.The colonization of Candida is prevalent, as well as the inflammatory lesions. Thereis a lack of appropriate information and assisting programs to aged in order to guaranteebetter mouth health conditions.

Recobrimento radicular pela técnica de Zucchelli e De Sanctis / Root covering by the technical Zucchelli and De Sanctis

A recessão gengival é encontrada com certa frequência na periodontia. Clinicamente, apresenta-se pela exposição radicular causada por um deslocamento apical da posição da gengiva em relação à junção cemento/esmalte. A recessão pode ser localizada, afetando apenas um dente ou grupo de dentes ou generalizada, ocorrendo em toda a boca. Sua etiologia está relacionada a vários fatores, incluindo placa bacteriana, posição dos dentes na arcada, escovação errônea, oclusão traumática, inserção alta de freios ou inserções musculares, área de gengiva estreita e pressão labial, entre outros. Uma meta do tratamento periodontal é regenerar a perda do aparelho de inserção dos dentes. Portanto, tornou--se evidente, durante a última década, que diversos procedimentos regenerativos têm potencial para corrigir não só a recessão gengival, pelo aumento da largura e altura da gengiva inserida ou queratinizada, como também obter recobrimento radicular parcial ou completo. Portanto, o objetivo deste trabalho é apresentar um relato de caso de recobrimento radicular de múltiplas recessões em áreas estéticas pela técnica de Zucchelli e De Sanctis.

Gingival recession is a common finding in periodontics. Clinically, it consists of root exposure caused by apical displacement of the gingival margin in the cement-enamel junction. Recession may be localized, affecting only a tooth or group of teeth, or widespread, affecting the entire mouth. Its etiology is related to several factors, such as bacterial plaque, position of the teeth in the arch, incorrect brushing, traumatic occlusion, high frenum insertion or muscle insertions, narrow gingival area and lip pressure, among others. The goal of periodontal treatment is to regenerate the periodontium. Therefore, it became evident during the last decade that several procedures have the potential to regenerate not only gingival recession by increasing the width and height of the inserted or keratinized gums, but also to achieve partial or full root coverage. In this context, the objective of this work is to present a case report of the coverage of multiple recessions in aesthetic areas by the Zucchelli and De Sanctis technique.

Sensibilidade pós-operatória em restaurações diretas extensas utilizando sistemas adesivos convencionais atuais / Postoperative sensitivity in large direct restorations using new total etch adhesive systems

O objetivo deste trabalho clínico foi avaliar a sensibilidade pós-operatória após 2, 7, 180 e 365 dias em restaurações de resina composta realizadas em cavidades amplas e profundas em dentes posteriores empregando-se três sistemas adesivos convencionais atuais (condicionamento ácido total+adesivo). Foram realizados 60 procedimentos restauradores pelo mesmo operador, divididos em três grupos com 20 amostras em cada: SB - ADPER SingleBond 2 (3M ESPE®); AB - All Bond 3 (Bisco®); e XP - XP Bond (Dentsply®). Todas as restaurações foram preenchidas com a resina Z350 (3M ESPE®) e os resultados foram submetidos aos testes de Kruskall Wallis e Friedman. Todos os resultados para sensibilidade pós-operatória foram semelhantes ao longo dos tempos testados.

Avaliação in vitro da atividade antifúngica de óleos essenciais e extratos de plantas da região amazônica sobre cepa de Candida albicans / In vitro evaluation of the anti-fungii activity of essential oils and plant extracts present in the amazon region about the strain of Candida albicans

A candidíase é uma doença fúngica oportunista causada pela proliferação de espécies de Candida, principalmente Candida albicans, sendo esta a espécie mais patogênica em humanos. Muitos antifúngicos existentes no mercado apresentam efeitos colaterais indesejáveis ou podem induzir a resistência fúngica, principalmente em indivíduos imunodeprimidos. Nessa perspectiva, o objetivo desta pesquisa foi avaliar in vitro a atividade antifúngica de óleos e extratos vegetais extraídos de plantas da região Amazônica e determinar a concentração inibitória mínima das espécies que apresentaram atividade antifúngica frente à cepa padrão de Candida albicans (ATCC 90028). A atividade antifúngica dos óleos essenciais Copaifera multijuga, Carapa guianenses, Piper aduncum e Piper hispidinervum foi avaliada pelo método de difusão em meio sólido em diluições de 32 a 2%, para determinação da concentração inibitória mínima. Os extratos de Annona glabra, Azadiractha indica, Bryophyllum calycinum, Eleutherine plicata, Mammea americana, Psidium guajava e Syzygium aromaticum foram testados nas concentrações de 500, 250, 125 e 62,5 mg.mL-1 e a atividade antifúngica foi verificada pelo método de difusão em meio sólido, utilizando-se discos de papel filtro. Os óleos testados não apresentaram efeito antifúngico sobre a cepa de Candida albicans e, dos extratos testados, somente os de Eleutherine plicata, Psidium guajava e Syzygium aromaticum apresentaram atividade antifúngica, com concentração inibitória mínima, respectivamente, de 250, 125 e 62,5 mg.mL-1. A partir dos resultados apresentados, verificou-se que os extratos de Eleutherine plicata, Psidium guajava e Syzygium aromaticum apresentam potencial efeito inibitório para crescimento de Candida albicans, servindo de guia para a seleção de plantas com atividades antifúngicas para futuros trabalhos de âmbito toxicológico e farmacológico.

Candidiasis is an opportunistic fungus disease caused by the proliferation of Candida species, mainly Candida albicans, what seems to be the most pathogenic one to humans. Several anti-fungii drugs available in the market present undesirable side effects or develop resistance, mainly in immune-depressed individuals. Hence, the aim of this paper was to evaluate in vitro the anti-fungii activity of vegetal oils and extracts present in the Amazon region and to determine the minimal inhibitory concentration of such species that have presented anti-fungii activity against the pattern strain of Candida albicans (ATCC 90028). The anti-fungii activity of the essential oils Copaifera multijuga, Carapa guianenses, Piper aduncum and Piper hispidinervum was determined through the method of difusion in a solid means and in dilutions of 32 to 2% in order to determine its minimal inhibitory concentration. The extracts Annona glabra, Azadiractha indica, Bryophyllum calycinum, Eleutherine plicata, Mammea americana, Psidium guajava and Syzygium aromaticum have been tested under the concentrations of 500, 250, 125 and 62,5 mg.mL-1 and the anti-fungii activity was determined through the method of difusion in a solid means using discs of flter paper. The tested oils did not present any anti-fungii effect on the strain of Candida albicans and, from the extracts tested, only Eleutherine plicata, Psidium guajava and Syzygium aromaticum have presented anti-fungii activity with minimal inhibitory concentrations, respectively, 250, 125 and 62,5 mg.mL-1. With the results presented, the extracts of Eleutherine plicata, Psidium guajava and Syzygium aromaticum present a potential inhibitory effect to the growth of Candida albicans, acting as a guide for the selection of plants with anti-fungii activities for further papers on a toxicologic and pharmacologic.

Genetic diversity and exoenzyme activities of Candida albicans and Candida dubliniensis isolated from the oral cavity of Brazilian periodontal patients.

OBJECTIVE: Mucosal surfaces are the primary oral reservoirs of Candida species, but these species can also be found in subgingival biofilm. The present study investigated the genetic diversity and production of exoenzymes of C. albicans and C. dubliniensis isolated from the oral cavity of systemically healthy patients with periodontitis. DESIGN: Fifty-three patients were analysed. Samples were collected from three oral cavity sites (periodontal pocket, gingival sulci and oral mucosa), plated and, after isolation, suspect strains of C. albicans and C. dubliniensis were identified by PCR. The genetic diversity of the isolates was evaluated by RAPD and the activities of the secreted aspartyl proteinases and phospholipases were evaluated by the agar plate method. RESULTS: Twenty-one patients showed positive results for Candida spp. There were no statistically significant differences between genders, or between sites. C. albicans was the most frequently found specie, while C. dubliniensis was isolated from the periodontal pocket of only one patient. Sixteen genotypes were detected among the C. albicans isolates, and one among the C. dubliniensis isolates. The similarity coefficient (S(SM)) values among the C. albicans genotypes ranged from 0.684 to 1.0 with an average of 0.905+/-0.074. All isolates produced high levels of Saps and most of them produced high levels of phospholipases. No relationship was found between the genotypes and the pattern of enzymatic production. There was no association between specific genotypes and their site of isolation. CONCLUSIONS: The results of the present study suggest that genetically homogeneous strains of C. albicans are present in the oral cavity of patients with periodontitis and that these strains are capable of producing high levels of exoenzyme.