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1.
Virology ; 525: 192-199, 2018 12.
Artigo em Inglês | MEDLINE | ID: mdl-30292963

RESUMO

Domestic dogs share habitats with human, a fact that makes them a potential source of zoonotic viruses. Moreover, knowledge regarding possible bloodborne pathogens is important due to the increasing application of blood transfusion in dogs. In the present study, we evaluated the serum virome of 520 dogs using throughput sequencing (HTS). The serum samples were pooled and sequenced using an Illumina MiSeq platform. Our unbiased method identified prevalent canine pathogens as canine protoparvovirus 1 (canine parvovirus 2), undersearched agents as canine bocaparvovirus 1 (minute virus of canines) and canine circovirus, circular viruses closely related to viruses recently found in human samples, and new parvovirus and anelloviruses. The dog virome described in the present work furthers the knowledge concerning the viral population in domestic animals. The present data includes information regarding viral agents that are potentially transmitted through blood transfusion among dogs.


Assuntos
Doenças do Cão/virologia , Viroses/veterinária , Vírus/isolamento & purificação , Animais , Brasil/epidemiologia , Doenças do Cão/sangue , Doenças do Cão/epidemiologia , Cães , Viroses/sangue , Viroses/epidemiologia , Viroses/virologia , Vírus/classificação
2.
Arch Virol ; 163(11): 3203-3206, 2018 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-30097743

RESUMO

A novel polyomavirus (PyVs) comprising 5,422 bp was identified by high-throughput sequencing (HTS) in pooled organs of nutria (Myocastor coypus). The new genome displays the archetypal organization of PyVs, which includes open reading frames for the regulatory proteins small T antigen (sTAg) and large T antigen (LTAg), as well as for the capsid proteins VP1, VP2 and VP3. Based on the International Committee on Taxonomy of Viruses (ICTV) Polyomaviridae Study Group criteria, this genome comprises a new PyVs species for the Alphapolyomavirus genus and is putatively named "Myocastor coypus Polyomavirus 1" . The complete genome sequence of this Myocastor coypus Polyomavirus 1 (McPyV1) isolate is publically available under the GenBank accession no. MH182627.


Assuntos
Infecções por Polyomavirus/veterinária , Polyomavirus/isolamento & purificação , Doenças dos Roedores/virologia , Roedores/virologia , Animais , Antígenos Virais de Tumores/genética , Proteínas do Capsídeo/genética , Genoma Viral , Sequenciamento de Nucleotídeos em Larga Escala , Fases de Leitura Aberta , Filogenia , Polyomavirus/classificação , Polyomavirus/genética , Infecções por Polyomavirus/virologia , Ratos
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