Avaliação do efeito da água de piscina sobre a rugosidade superficial de resinas compostas de esmalte / Evaluation of the pool water effect on the surface roughness of enamel composite resins

Introdução: a estabilidade de cor e a durabilidade de uma restauração em resina composta são influenciadas por sua lisura superficial. Características inerentes ao material, procedimentos de acabamento e polimento, dieta e hábitos dos indivíduos podem interferir nas suas propriedades superficiais. Objetivo: avaliar a rugosidade superficial de resinas compostas de esmalte submetidas ao contato com água de piscina clorada e estimar se o uso de uma proteção mecânica limitaria esse contato e seus efeitos. Metodologia: duzentos corpos de prova, de cinco diferentes resinas foram preparados e distribuídos, aleatoriamente, em quatro grupos (n=10), de acordo com o desafio a que foram submetidos: imersão em água destilada sem e com proteção de moldeira de acetato; imersão em água clorada sem e com proteção de moldeira de acetato. Os corpos ficaram imersos por 30 dias, sendo as respectivas águas trocadas diariamente. Para avaliação da rugosidade superficial, utilizou-se um rugosímetro. Procedeu-se à análise estatística inferencial por meio da análise de variância a 3-critérios, avaliando-se a comparação múltipla dessas médias com auxílio do teste de Tukey (nível de significância de 5%). Resultados: observou-se: interação significativa entre os fatores água e condição experimental (p<0,0001) e maior rugosidade superficial para a resina Esthet-X HD, quando exposta à água clorada. O uso da moldeira resultou em maiores valores de rugosidade superficial para todas as resinas testadas quando expostas à água clorada. Conclusão: o efeito da água de piscina foi material dependente. O uso da proteção mecânica pareceu aumentar a rugosidade superficial das resinas compostas expostas à água clorada.

Introduction: the color stability and durability of a composite resin restoration are influenced by its surface smoothness. Characteristics inherent to the material, finishing and polishing procedures, diet and habits of individuals may interfere with their surface properties. Objective: to evaluate the surface roughness of enamel composite resins submitted to contact with chlorinated pool water and to estimate if the use of a mechanical protection would limit this contact and its effects. Methodology: two hundred specimens of five different resins were prepared and randomly distributed into four groups (n = 10), according to the challenge they were subjected to: immersion in distilled water, without and with acetate tray protection; immersion in chlorinated water, without and with acetate tray protection. The bodies were immersed for 30 days, and their waters were changed daily. To evaluate the surface roughness, a rugosimeter was used. Inferential statistical analysis was performed using 3-criteria analysis of variance, and a multiple comparison of these means was evaluated using Tukey's test (significance level of 5%). Results: it was observed a significant interaction between water and experimental conditions (p <0.0001) and greater surface roughness for Esthet-X HD resin when exposed to chlorinated water. The use of the tray resulted in higher surface roughness values for all the resins tested when exposed to chlorinated water. Conclusion: the effect of pool water was dependent material. The use of mechanical protection appeared to increase the surface roughness of composite resins exposed to chlorinated water.

An in vitro and in vivo study of the α-amylase activity of phaseolamin.

We evaluated the polypeptide profiles, inhibition of human salivary α-amylase activity, and hemagglutination properties of a commercial phaseolamin sample. We also performed an in vivo assay to investigate the effects of a commercial phaseolamin treatment (100, 500, or 1500 mg/kg) over 20 days on the glycemia, body weight, and serum biochemical parameters (total cholesterol, triglycerides, alanine aminotransferase, and aspartate aminotransferase) of nondiabetic and streptozotocin-induced diabetic rats. The in vitro evaluation showed defined protein profiles, low hemagglutination activity, and high α-amylase inhibition. None of the experimental groups treated with phaseolamin or acarbose showed decreases in body weight. Our data demonstrate that phaseolamin inhibits amylase activity in vitro, reduces blood glucose levels, decreases or attenuates some of the renal and hepatic effects of diabetes in streptozotocin-induced rats, and could therefore have therapeutic potential in the treatment or prevention of the complications of diabetes.

Overexpression of myosin-IIB in the brain of a rat model of streptozotocin-induced diabetes.

The Ca(2+)/calmodulin complex interacts with and regulates various enzymes and target proteins known as calmodulin-binding proteins (CaMBPs). This group of proteins includes molecular motors such as myosins. In this study, we show that non-muscle myosin-IIB is overexpressed in the brains of diabetic rats. We isolated CaMBPs from the brains of non-diabetic rats and rats with streptozotocin-induced diabetes and purified them by immobilized-calmodulin affinity chromatography. The proteins were eluted with EGTA and urea, separated by SDS-PAGE, digested and submitted to peptide mass fingerprinting analysis. Thirteen intense bands were found in both types of brains, two were found exclusively in non-diabetic brains and four were found exclusively in diabetic brains. A large fraction of the eluted proteins contained putative IQ motifs or calmodulin-binding sites. The results of the myosin-IIB affinity chromatography elution, western blot and RT-PCR analyses suggest that myosin-IIB protein and mRNA are expressed at high levels in diabetic brains. This is the first study that has demonstrated differential expression of CaMBPs in diabetic and non-diabetic brain tissue through a comparative proteomic analysis, and it opens up a new approach to studying the relationship between the expression of myosins in the brain, hyperglycemia and intracellular calcium regulation.