Effect of antimicrobial photodynamic therapy on the reduction of bacteria and virulence factors in teeth with primary endodontic infection.

OBJECTIVE: To evaluate the effectiveness of supplemental photodynamic therapy for improving the bacterial removal and the levels of lipopolysaccharide (LPS) and lipoteichoic acid (LTA) by conducting a clinical trial. METHODOLOGY: Twenty-four root canals with pulp necrosis and periapical lesion were selected and randomly divided into conventional group using endodontic treatment with chemo-mechanical preparation (CMP) alone (n = 12) and a group using antimicrobial photodynamic therapy (aPDT) after CMP (n = 12). The samples were collected before and after CMP (conventional group) and after photodynamic therapy (aPDT group). A photosensitizer (0.005% methylene blue) was applied to the root canal for 3 minutes after CMP, whereas aPDT was performed by using a red laser with a power of 30Mw and energy density of 9J/cm2 for 90 s per root canal. Culture technique was performed to determine the bacterial colony forming units. LPS and LTA levels were quantified by using limulus amoebocyte lysate (LAL) assay and enzyme-linked immunosorbent assay (ELISA), respectively. RESULTS: All samples showed growth of viable bacteria on Fastidious Anaerobe Agar (FAA), with an average of 5.19 × 105 CFU/ mL. CMP was effective in decreasing viable bacteria (p < 0.05), whereas there was a significant decrease (p < 0.05) in the samples treated with aPDT compared to those submitted to CMP. LPS and LTA were detected in all initial samples, with mean values of 20.561 EU/mL and 430.91 pg/mL, respectively. Both CMP and aPDT groups significantly decreased the levels of LPS and LTA (p < 0.05), with a statistical difference between the groups regarding aPDT (p < 0.05). CONCLUSION: Photodynamic therapy as an adjunct to CMP proved to be effective in improving root canal disinfection and reducing the LPS and LTA levels in teeth with primary endodontic infection.

The effect of photodynamic therapy on postoperative pain in teeth with primary endodontic infection.

INTRODUCTION: The objective of this study was to investigate the action of photodynamic therapy on pain control after endodontic treatment in asymptomatic teeth with a primary infection, within a single visit. METHODS: Sixty (60) single-rooted teeth with pulp necrosis and periapical lesions were selected and randomly divided into two (2) groups (n = 30), according to the protocol; a control group (CG) and a group using photodynamic therapy (aPDT). The canals were instrumented with Reciproc files # 25 up to 40 along the entire length of the canal, using 2% chlorhexidine gel as the auxiliary chemical substance, followed by irrigation with sterile saline. aPDT consited of 0.005% methylene blue as photosensitizer, using AsGaAl diode laser, 660 nm wavelength, 100 mW of power and 9 J of energy, using optical fibers with 365 µm in diameter. The canals were filled with Endomethasone N cement. RESULTS: Pain intensity was assessed at 8, 12, 24, 48, 72 h and 1 week after endodontic treatment using a visual analogue scale. The level of pain was classified as none (0), mild (1-3), moderate (4-7) or severe (8-10). The data were at a significance level of 5%. There was a statistically significant difference (p<0.05) in the periods of 8, 12, 24, 48 and 72 h between the control group and the aPDT group. After 1 week, there was no statistically significant difference. CONCLUSIONS: It is concluded that photodynamic therapy had a significant effect on decreasing post-endodontic treatment pain in teeth with necrotic pulp and asymptomatic periapical lesions.

Condutas práticas e efetivas do cirurgião-dentista frente ao paciente em tratamento de câncer de cabeça e pescoço: revisão de literatura / Practical and effective conducts of the dental surgery in front of the patient in treating head and neck cancer: literature review

O objetivo do presente estudo é salientar através de uma revisão de literatura os possíveis cuidados que o cirurgião dentista precisa ter frente aos principais agravos bucais decorrentes do tratamento oncológico. Os trabalhos foram obtidos através dos sistemas de dados PUBMED, MEDLINE, BBO, SCIELO, LILACS, SCIENCE DIRECT, COCHRANE E BBO. A principal estratégia para o combate de tais neoplasias é na forma de prevenção, ainda que o câncer tenha como um dos fatores etiológicos a hereditariedade, a exposição a determinados agentes (fumo, álcool, HPV, etc.) podendo aumentar as chances de sua ocorrência. Os métodos tradicionais de tratamento oncológico são a cirurgia, a radioterapia e a quimioterapia. O tratamento a ser instituído estará na dependência da localização, do grau de malignidade e da condição de saúde do indivíduo. O cuidado dentário antes do tratamento oncológico visa a redução do risco e a gravidade das complicações orais, prevenir, eliminar ou reduzir a dor de origem bucal, preservar ou melhorar a saúde bucal, contribuindo para a melhoria da qualidade de vida. Conclui-se que diante dos efeitos colaterais importantes aos tecidos bucais, é de suma necessidade o conhecimento por parte do cirurgião dentista, para que possa atuar na prevenção e redução de tais danos(AU)

The aim of the present study is to highlight a literature review on the possible treatments that the dental surgeon needs to face the main oral health problems caused by cancer treatment. The work was carried out through the data systems PUBMED, MEDLINE, BBO, SCIELO, LILACS, CIÊNCIA DIRETA, COCHRANE AND BBO. The main strategy to combat these neoplasms is the form of prevention, but cancer has inherited etiological factors, exposure to dangerous agents (smoking, alcohol, HPV, etc.) that can increase the chances of its occurrence. Traditional methods of cancer treatment are surgery, radiation and chemotherapy. Treatment is instituted depending on the individual's location, degree of malignancy and health condition. Dental care before cancer treatment reduces the risk and severity of oral complications, prevents, eliminates or reduces pain of oral origin, preserves or improves oral health, contributes to improving quality of life. It is concluded that in view of the important effects on oral tissues, knowledge or knowledge on the part of the dental surgeon is necessary, so that it can perform the prevention and reduction of such damages(AU)