Influence of contemporary ceramo-metallic crown decontamination on the surface roughness, color stability, and disinfection. An in vitro analysis.

AIM: To assess the antimicrobial effects, surface roughness (Ra), and color change (ΔE) of Morinda Citrofolia, Sapindus Mukorossi, Indocyanine green photosensitizer, and 2% NaOCl on metal ceramic crown colonized with C. Albicans, S aureus, S. mutans, and E. coli. MATERIAL AND METHODS: An aggregate of 40 (n = 10 each) Ni/Cr metal-ceramic crowns were fabricated and inoculated with the American Type Culture Collection (ATCC) of varied microbial colonies colonized by C. Albicans, S aureus, S. mutans, and E. coli. After inoculation, specimens were exposed to four different types of disinfection methods i.e. group 1: MC, group 2: SM, group 3:ICGP, and group 4 2% NaOCl respectively to evaluate antimicrobial efficacy, Ra, and color change. Later, the microbial assay was performed and expressed as CFU/ml (log 10). Ra was assessed using a surface analyzer and color change ΔE was evaluated using a spectrophotometer Statistical analysis was performed by using two-way ANOVA and Post HocTukey's test maintaining a significance level of p less than 0.05. RESULTS: The highest antimicrobial efficacy for metallic-ceramic crown decontamination against all inspected microbial colonies was unveiled by group 1: MC, whereas the lowest disinfection efficacy was revealed by group 4 sterilized with 2% NaOCl (control). Metallic ceramic crown decontamination with MC and SM substantiated equivalent antimicrobial efficacy in plummeting CFU/mL (log10) of C.Albicans, S aureus, S.mutans, and E. coli (p>0.05). However, ICGP as a crown disinfectant validated antimicrobial efficacy significantly less compared to MC and SM (p<0.05). Ra was assessed post-disinfection with no changes observed (p>0.05) among all groups. Similarly, ΔE was significantly higher in crowns disinfected with ICGP (p<0.05) CONCLUSION: Metallic ceramic crown decontamination using herbal disinfectants Morinda Citrofolia and Sapindus Mukorossi exhibited favorable antimicrobial efficacies against microbial colonies of C. albicans, S aureus, S.mutans, and E. coli with no changes in surface roughness and color.

Effect of contemporary retraction agents and cleaning with hydrogen peroxide on the polymerization of elastomeric impression materials.

The aim of the study is to investigate the polymerization inhibition of elastomeric impression materials vinylpoly siloxane (VPS) and polyether (PE) when used in combination with retraction materials with and without subsequent cleaning with hydrogen peroxide (H2O2). Methods: Seven stainless steel specimens were fabricated. Four hundred and twenty impressions were made with three different elastomeric materials (140 each) as follows: group 1: VPS-Panasil; group 2: VPS-Express; group 3: PE-Monophase. Each material group was further subdivided into seven subgroups, based on use of no retraction material (control), three different retraction materials [Retraction capsule (RC3M), Dryz, Expasyl], and two cleaning techniques (water and H2O2). All subgroups included 20 impressions, which were made by a single operator using an automix gun. Evaluations were made using a visual scale by three calibrated examiners blindly and independently. Subjective categorization of the impressions were made as inhibited and uninhibited. Data were analyzed using Fisher's exact test and significance was set at p < 0.05. Results: Inhibited impressions were lower than uninhibited impressions among VPS materials (Panasil and Express); Panasil and Express showed comparable (p > 0.05) impression retardation. PE showed significantly higher inhibition compared to VPS materials (p < 0.05). Expasyl showed significantly higher polymerization inhibition than other retraction materials (p < 0.05). The use of H2O2 for cleaning showed significant reduction in polymerization inhibition than cleaning with water for Expasyl (p < 0.05). Conclusion: Overall contemporary retraction materials showed low potential for polymerization inhibition of elastomeric impression materials. Expasyl should be cleaned with H2O2 prior to impression making. However, Dryz and RC3M can show accurate impressions with water cleaning alone.

Developing and establishing research guidelines in a private higher education institution of Saudi Arabia. An experience.

OBJECTIVES: To develop and establish research guidelines of international standards that matches appropriate cross-cultural and religious considerations of the region. METHODS: We followed the guidelines given by the American College of Cardiology Foundation and American Heart Association, Inc., Washington DC, USA task force on practice to develop research guidelines of international standards for our institution. In October 2015 the task team (of a private higher education institution in Jeddah, Saudi Arabia) qualitatively analyzed the national codes and guidelines using 19 ethical protections stated in the other universally accepted international guidelines. RESULTS: The electronic search yielded 11 different guidelines and documents, of which 4 documents were from Saudi Arabia. When the Saudi Arabian documents were compared using 19 universally accepted ethical protections, we found one of the document contained 15 of the 19 ethical protections, another document contained 9 protections and the least being 5 of the 19 protections. CONCLUSION: Research guidelines of international standards were developed with respect to the cultural, traditional, and religious considerations of the Kingdom, providing a valuable framework to guide our institutional researchers to conduct ethically sound inquiry.