Your browser doesn't support javascript.
loading
Mostrar: 20 | 50 | 100
Resultados 1 - 5 de 5
Filtrar
Mais filtros










Base de dados
Intervalo de ano de publicação
1.
Prion ; 9(1): 29-33, 2015.
Artigo em Inglês | MEDLINE | ID: mdl-25730397

RESUMO

Here we report a genetically confirmed case of Creutzfeldt-Jakob disease with a prion protein gene codon 180 mutation presenting atypical magnetic resonance imaging findings. The present case exhibited an acute onset and lateralized neurologic signs, and progressive cognitive impairment. No myoclonus or periodic synchronous discharges on electroencephalography were observed. Diffusion-weighted images revealed areas of high signal intensity in the right frontal and temporal cortices at onset that extended to the whole cortex and basal ganglia of the right cerebral hemisphere at 3 months. Although the cerebrospinal fluid (CSF) was initially negative for neuron specific enolase, tau protein, 14-3-3 protein, and abnormal prion protein, the CSF was positive for these brain-derived proteins at 3 months after onset.


Assuntos
Córtex Cerebral/patologia , Códon/genética , Síndrome de Creutzfeldt-Jakob/genética , Imageamento por Ressonância Magnética , Mutação/genética , Príons/genética , Idoso , Córtex Cerebral/diagnóstico por imagem , Síndrome de Creutzfeldt-Jakob/diagnóstico por imagem , Humanos , Masculino , Tomografia Computadorizada de Emissão de Fóton Único
3.
Waste Manag ; 30(7): 1161-4, 2010 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-19963363

RESUMO

Refuse-derived fuel (RDF)-RDF-5 according to ASTM guidelines-derived from municipal solid waste and other waste materials, has been prepared as a fuel source for power plants in Japan. RDF has been known as a stable and safe solid-fuel. However, some spontaneous ignition incidents occurred during storage of RDF in certain facilities. In the present study, the storage conditions, which might induce the spontaneous ignition in RDF samples, was investigated to understand and prevent this phenomenon. When the initial temperature of RDF sample was consistent, higher water content and shorter induction times was observed (e.g., the induction times of select RDF samples with 5.8% and 16.7% water content, was 446 and 270 min, respectively). Also, the induction time was affected by the size of a RDF sample. No relationship between bacterial fermentation and spontaneous ignition was observed. The linear relationship between the induction time and the inverse of the initial temperature of the RDF sample was obtained in the Arrhenius equation.


Assuntos
Centrais Elétricas , Resíduos , Incineração , Eliminação de Resíduos , Gerenciamento de Resíduos
4.
Biosci Biotechnol Biochem ; 73(3): 702-9, 2009 Mar 23.
Artigo em Inglês | MEDLINE | ID: mdl-19270381

RESUMO

A lectin, PPL, was purified from the mushroom Pleurocybella porrigens. The results of SDS-PAGE, gel filtration, and MALDI-TOF-mass of PPL indicated that its molecular mass was 56 kDa, and it was composed of four 14 kDa subunits with no disulfide bonds. In hemagglutination inhibition assay, PPL exhibited the strongest binding specificity toward GalNAc among the mono- and oligo-saccharides tested. Among the glycoproteins, asialo-bovine submaxillary mucin (asialo-BSM) showed the strongest inhibitory effect. In surface plasmon resonance analysis, asialo-BSM, porcine stomach mucin (PSM), and BSM exhibited potent binding affinity. The complete amino acid sequence was determined by amino acid sequencing of intact and of enzyme-digested PPL. The cDNA of PPL was cloned from RNA extracted from the mushroom. The open reading frame of the cDNA of the protein consisted of 411 bp, encoding 137 amino acids. This is the first report of isolation of a lectin of the genus Pleurocybella.


Assuntos
Agaricales/genética , DNA Complementar/genética , Lectinas/genética , Lectinas/isolamento & purificação , Sequência de Aminoácidos , Sequência de Bases , Clonagem Molecular , Glicoproteínas/metabolismo , Glicoproteínas/farmacologia , Hemaglutinação/efeitos dos fármacos , Concentração de Íons de Hidrogênio , Proteínas Imobilizadas/metabolismo , Cinética , Lectinas/química , Lectinas/metabolismo , Dados de Sequência Molecular , Monossacarídeos/farmacologia , Oligossacarídeos/farmacologia , Estabilidade Proteica , Temperatura
5.
Biochem Biophys Res Commun ; 313(4): 1044-52, 2004 Jan 23.
Artigo em Inglês | MEDLINE | ID: mdl-14706648

RESUMO

Cryptosporidium parvum is a parasitic protozoan that causes the diarrheal disease cryptosporidiosis, for which no satisfactory chemotherapy is currently available. Although the presence of mitochondria in this parasite has been suggested, its respiratory system is poorly understood due to difficulties in performing biochemical analyses. In order to better understand the respiratory chain of C. parvum, we surveyed its genomic DNA database in GenBank and identified a partial sequence encoding cyanide-insensitive alternative oxidase (AOX). Based on this sequence, we cloned C. parvum AOX (CpAOX) cDNA from the phylum apicomplexa for the first time. The deduced amino acid sequence (335 a.a.) of CpAOX contains diiron coordination motifs (-E-, -EXXH-) that are conserved among AOXs. Phylogenetic analysis suggested that CpAOX is a mitochondrial-type AOX, possibly derived from mitochondrial endosymbiont gene transfer. The recombinant enzyme expressed in Escherichia coli showed quinol oxidase activity. This activity was insensitive to cyanide and highly sensitive to ascofuranone, a specific inhibitor of trypanosome AOX.


Assuntos
Cryptosporidium parvum/enzimologia , Oxirredutases/metabolismo , Sequência de Aminoácidos , Animais , Sequência de Bases , Clonagem Molecular , Criptosporidiose/tratamento farmacológico , Cryptosporidium parvum/efeitos dos fármacos , Cryptosporidium parvum/genética , DNA de Protozoário/genética , Inibidores Enzimáticos/farmacologia , Humanos , Mitocôndrias/enzimologia , Dados de Sequência Molecular , Oxirredutases/antagonistas & inibidores , Oxirredutases/química , Oxirredutases/genética , Filogenia , Proteínas Recombinantes/antagonistas & inibidores , Proteínas Recombinantes/química , Proteínas Recombinantes/genética , Proteínas Recombinantes/metabolismo , Homologia de Sequência de Aminoácidos , Sesquiterpenos/farmacologia
SELEÇÃO DE REFERÊNCIAS
DETALHE DA PESQUISA
...