Corrigendum: Applied insight: studying reducing the carbon footprint of the drying process and its environmental impact and financial return.

[This corrects the article DOI: 10.3389/fbioe.2024.1355133.].

Applied insight: studying reducing the carbon footprint of the drying process and its environmental impact and financial return.

Harnessing solar energy is one of the most important practical insights highlighted to mitigate the severe climate change (CC) phenomenon. Therefore, this study aims to focus on the use of hybrid solar dryers (HSDs) within an environmentally friendly framework, which is one of the promising applications of solar thermal technology to replace traditional thermal technology that contributes to increasing the severity of the CC phenomenon. The HSD, based on a traditional electrical energy source (HSTEE) and electrical energy from photovoltaic panels (HSPVSE), was evaluated compared to a traditional electrical (TE) dryer for drying some medicinal and aromatic plants (MAPs). This is done by evaluating some of the drying outputs, energy consumed, carbon footprint, and financial return at 30, 40, and 50°C. The best quality of dried MAP samples in terms of essential oil (EO, %) and microbial load was achieved at 40°C. The HSTEE dryer has reduced energy consumption compared to the TE dryer by a percentage ranging from 37% to 54%. The highest CO2 mitigated ratio using the HSTEE dryer was recorded in thyme, marjoram, and lemongrass samples, with values ranging from 45% to 54% at 30, 40, and 50°C. The highest financial return obtained from energy consumption reduction and carbon credit footprint was achieved at 50°C, with values ranging from 5,313.69 to 6,763.03 EGP/year (EGP ≈ 0.0352 USD) when coal was used as a fuel source for the generation of electricity. Moreover, the HSPVSE dryer achieved a 100% reduction in traditional energy consumption and then reduced CO2 emissions by 100%, which led to a 100% financial return from both energy reduction and carbon credit. The highest financial returns were observed at 50°C, with values ranging from 13,872.56 to 15,007.02, 12,927.28 to 13,984.43, and 11,981.99 to 12,961.85 EGP/year (EGP ≈ 0.0352 USD) for coal, oil, and natural gas, respectively. The HS dryers show potential for environmental conservation contribution; furthermore, earning money from energy savings and carbon credit could help improve the living standards and maximize benefits for stakeholders.

Comparative studies of four cumin landraces grown in Egypt.

One of the significant aromatic plants applied in food and pharma is cumin. Despite its massive trading in Egypt, there are no comprehensive reports on cumin landraces profile screening. This study aimed to investigate the variation in seeds' physical and biochemical profiles and genetic diversity as well as assess the efficiency of seeds' germination under salinity stress. Consequently, during the 2020/2021 growing season, four common cumin seed landraces were gathered from various agro-climatic regions: El Gharbia, El Menia, Assiut, and Qena. Results showed a significant variation in physical profile among the four seeds of landraces. In addition, Assiut had the highest percentage of essential oil at 8.04%, whilst Qena had the largest amount of cumin aldehyde, the primary essential oil component, at 25.19%. Lauric acid was found to be the predominant fatty acid (54.78 to 62.73%). According to ISSR amplification, El Menia presented a negative unique band, whereas other landraces offered a positive band. Additionally, the cumin genotypes were separated into two clusters by the dendrogram, with El Gharbia being located in an entirely separate cluster. There were two sub-clusters within the other cluster: El Menia in one and Assiut and Qena in the other. Moreover, the germination sensitivity to the diverse salinity concentrations (control, 4, 8, 12, and 16 dS/m) findings showed that landraces exhibited varying responses to increased salinity when El Gharbia and El Menia showed a moderate response at four dS/m. Whilst, Qena landraces showed supreme values among other landraces under 12 and 16 dS/m. The majority of the examined features had strong positive associations over a range of salinity levels, according to phenotypic correlation coefficient analysis. To accomplish the aims of sustainable agriculture in Egypt, it would be imperative that the potential breeding program for cumin landraces consider this screening study.

Towards Sustainable Pest Management: Toxicity, Biochemical Effects, and Molecular Docking Analysis of Ocimum basilicum (Lamiaceae) Essential Oil on Agrotis ipsilon and Spodoptera littoralis (Lepidoptera: Noctuidae).

Over the last decade, essential oils (EOs) have become potential ingredients for insecticide formulations due to their widespread availability and perceived safety. Therefore, this study aimed to evaluate the toxicity and biochemical efficacy of basil (Ocimum basilicum) (Lamiaceae) against two destructive pests Noctuidae, Agrotis ipsilon (Hufnagel) and Spodoptera littoralis (Boisduval) (Lepidoptera: Noctuidae). In addition, a molecular docking study was performed to gain insight into the binding pattern between glutathione S-transferase (GST) and linalool, the main component of EO. GC-MS analysis of O. basilicum EO revealed that linalool is the most abundant compound (29.34%). However, the toxicity tests showed no significant difference between the values of LC50 of O. basilicum EO to A. ipsilon and S. littoralis. On the other hand, the sublethal experiments indicated that treating the second instar larvae with LC15 or LC50 values of O. basilicum EO significantly prolonged the larval duration in both insects, compared to the control. Regarding the biochemical effect of O. basilicum EO, the treatments significantly impacted the activity of detoxification enzymes. A notable elevation in glutathione S-transferase (GST) activity was recorded in A. ipsilon larvae compared with a reduction in S. littoralis larvae. The molecular docking analysis revealed that linalool bonded with the amino acid serine (SER 9) of GST, indicating its binding affinity with the enzyme. The obtained results could offer valuable insights into the mode of action of O. basilicum and can encourage the adoption of sustainable pest control practices that incorporate essential oils.

Development and evaluation of a hybrid smart solar dryer.

A hybrid smart solar dryer (HSSD) based on indirect forced convection and a controlled auxiliary heating system was developed, fabricated, and tested to be convenient for sunny and cloudy weather conditions. The achievements of the developed dryer focus on controlling the temperature of the dryer, increasing the drying rate, reducing energy consumption, and providing high-quality products. The HSSD was tested and evaluated for drying basil and sage herbs at 30, 40, and 50°C. The results showed that the fresh basil and sage leaves of 1 kg with a moisture content of 84.7% and 75.53% (wet basis) were dried within 58, 46, 32 and 38, 28, and 20 h at 30, 40, and 50°C, respectively. Correspondingly, the traditional drying methods achieved 96 h outdoors and 144 h indoors at room temperature. The average of the fabricated flat-plate solar collector efficiency (thermal efficiency, ηfpsc ) was ranged from 49.18% ± 9.52% to 66.02% ± 2.8%. The highest drying rates were achieved with the HSSD method. Moreover, the HSSD method led to a remarkable saving in energy with values ranging from 25.54% to 77.1% versus the traditional drying methods. While the best quality in terms of essential oil content and microbial load for the dried basil and sage herbs was achieved by the HSSD at 40°C. Finally, the HSSD is a promising energy-efficient method where it can save 70% of energy consumption, thus reducing the carbon footprint of drying processes, and providing higher quality products compared to the conventional methods.

Insights into the toxicity, biochemical activity, and molecular docking of Cymbopogon citratus essential oils and citral on Spodoptera littoralis (Lepidoptera: Noctuidae).

Insecticide resistance is a significant problem in insect management that can result from several processes including target-site change and increased activity of detoxifying enzymes. Spodoptera littoralis is one of the most resistant insect pests. For more effective insect management, alternatives to synthetic pesticides are encouraged. One of these alternatives is essential oils (EOs). Cymbopogon citratus EO and its main constituent citral were, therefore, considered in this study. The results revealed that C. citratus EO and citral exhibited significant larvicidal activity against S. littoralis, and the former was insignificantly more toxic than the latter. Additionally, treatments significantly affected the activity of detoxification enzymes. Cytochrome P-450 and glutathione-S-transferase were inhibited, while carboxylesterases, a-esterase and ß-esterase, were induced. The molecular docking study indicated that citral bonded with the amino acids cysteine (CYS 345) and histidine (HIS 343) of cytochrome P-450. This result suggests that interaction with cytochrome P-450 enzyme is one key mechanism by which C. citratus EO and citral act in S. littoralis. The results of our study are hoped to contribute to a better understanding of the mechanism of action of essential oils at the biochemical and molecular levels and provide safer and more efficient pest management solutions for S. littoralis.

Correction: Moustafa et al. Insecticidal Activity of Lemongrass Essential Oil as an Eco-Friendly Agent against the Black Cutworm Agrotis ipsilon (Lepidoptera: Noctuidae). Insects 2021, 12, 737.

Additional Affiliations [...].

Insecticidal Activity of Lemongrass Essential Oil as an Eco-Friendly Agent against the Black Cutworm Agrotis ipsilon (Lepidoptera: Noctuidae).

BACKGROUND: The destructive insect pest Agrotis ipsilon (Hufnagel) (Lepidoptera: Noctuidae) is a polyphagous species targeting many economically important plants. The extensive and arbitrary use of insecticides has resulted in the build-up of insecticide resistance and pesticide residues accumulating in food. Therefore, it is becoming evident that alternative pest management tools are needed to reduce risks to humans, the environment, and non-target organisms, and at the same time, they should be used in field application at the lowest cost. METHODS: In view of this objective, the present study demonstrates the toxicity of lemongrass (Cymbopogon citratus (DC.) Stapf) essential oil (EO), against the black cutworm A. ipsilon under controlled laboratory conditions in terms of measuring the activity of peroxidase and detoxification enzymes. The chemical components of the EO were analyzed using GC-MS. RESULTS: The results show that after 96 h post treatment, the LC15 and LC50 values were 427.67 and 2623.06 mg/L, respectively, of C. citratus EO on second-instar larvae of A. ipsilon. A slight significance in elongation of the larval duration with LC15 and LC50 value was found with control. By GC-MS analysis, the main compounds identified in the EO were α-citral and ß-citral with percentages of 35.91%, and 35%, respectively. The oxidative stress indicates a significant increase in CAT and lipid peroxidase enzyme activity after 96 h post treatment at the LC15 and LC50. Conversely, the detoxification enzyme activity shows an inhibition of CarE and GST enzymes of larvae exposed to LC15 and LC50 values in response to C. citratus EO. CONCLUSIONS: The present data show that lemongrass EO has insecticidal activity against the black cutworm, A. ipsilon.

Comparative studies of eco-friendly compounds like humic acid, salicylic, and glycyrrhizic acids and their nanocomposites on French basil (Ocimum basilicum L. cv. Grand verde).

As the green tactics for enhancing plant growth and production using naturally occurring materials are highly needed, it is important to use the nanoformulation of these materials as an attractive novel technique. Therefore, this research has been performed to evaluate the plants' morphological traits, the qualitative parameters, and molecular genetic characteristics using random amplified polymorphic DNA (RAPD) of French basil independence on growth biostimulators and their nanocomposite. The treatments included normal formulations and nanocomposite formulation of humic acid (5 mM HA), salicylic acid (1.4 mM SA), and glycyrrhizic acid ammonium salt (0.4 mM GA) and control treatment (water application). The results show that foliar spray with HA, SA, GA, and their nanocomposites significantly increased (p ≤ 0.05) on all vegetative growth characters, photosynthetic pigments, oil yield/plant, mineral content, and antibacterial activity as compared with control plants. Also, 1,1-diphenyl-2-picrylhydrazyl (DPPH) values of different samples used varied from 70.63 to 74.93%, with a significant increase compared to untreated plants. The most marked increases have been observed in treated plants with biostimulants in the nanocomposites form than in the natural form. On the other hand, GA and its nanocomposite showed variable effects on basil plants and gave the lowest increase values in all parameters than the other biostimulant but have high antimicrobial activity. For the molecular study, ten selected primers displayed a total of 288 amplified fragments scored per primer ranging from 7 to 46 fragments; 157 bands were polymorphic with 69% polymorphism. It could be concluded that humic acid and its nanocomposite are the most effective biostimulants that increased plant productivity and oil content.

Comment on: Adrenal hypoplasia congenita in identical twins.

[No Abstract Available].

Adrenal hypoplasia congenita in identical twins.

We are presenting a monozygotic twin brothers presented at different ages with different presentations. Twin-A presented at age of 18 days with salt losing crisis. Investigations revealed high plasma renin with low-normal aldosterone. Adrenocorticotropic hormone, stimulation test revealed low 17-OH progesterone at 0 and 60 minutes. Adrenocorticotropic hormone level and serum cortisol were normal, which excluded initial impression of congenital adrenal hyperplasia. He was diagnosed to have isolated primary hypoaldosteronism. At age of 18 months, he was noticed to have hyperpigmentation of lips and gum. Adrenal failure was suspected, and hydrocortisone was added. Twin-B presented at 9 years and 6 months of age with adrenal crisis. Both were having unilateral undescended testes. Adrenal hypoplasia congenita (AHC) was suspected after his twin's presentation.  Molecular analysis for gene study for both of them revealed adrenal insufficiency, NR0B1 (DAX1) gene mutation. In conclusion, gene analysis is important for the diagnosis of AHC and for genetic counseling.