1.
Chem Biol
; 10(11): 1115-27, 2003 Nov.
Artigo
em Inglês
| MEDLINE
| ID: mdl-14652079
RESUMO
The quantitative analysis of protein-protein and protein-peptide complexes is of fundamental importance in biochemistry. We report here that nickel-catalyzed proximity biotinylation and Ru(II)(bpy)(3)(2+)-mediated oxidative crosslinking can be used to measure the equilibrium dissociation constant and stoichiometry of protein complexes. Only small amounts of protein are required, neither of the binding partners must be immobilized on a surface, and no special instrumentation is necessary. This chemistry should provide a useful complement to existing methods for the analysis of protein-protein and protein-peptide interactions.
Assuntos
Peptídeos/química , Proteínas/química , Sequência de Aminoácidos , Sequência de Bases , Biotina/química , Catálise , Primers do DNA , Dados de Sequência Molecular , Níquel/química , Oxirredução
2.
Angew Chem Int Ed Engl
; 41(2): 356-9, 2002 Jan 18.
Artigo
em Inglês
| MEDLINE
| ID: mdl-12491432