Callosobruchus maculatus males and females respond differently to grandparental effects.

In this study, we used the cowpea weevil Callosobruchus maculatus (Coleoptera: Chrysomelidae) and two essential oils (mint and rosemary) to investigate the effect of the parents (F0) exposure to a sublethal dose of essential oil on grand offspring (F2) encountering the same essential oil. Then we evaluated biological parameters, including immature development time, sex ratio, adult emergence, egg number, egg hatch, longevity, and mating behaviors in three generations (F0, F1, and F2). Results showed when F0 experienced essential oil in the embryonic stage, parental and grandparental effects were more severe than adulthood experiences. Also, grandparental effects increased or decreased reactions of F2 generation when faced with a similar essential oil, depending on grand offspring sex. For example, when grandparents experienced rosemary essential oil in the embryonic stage, they produced more tolerant female grand offspring with a better ability to cope with the same essential oil (increased adult longevity and egg number). However, male grandoffspring were more sensitive (had a higher mortality percentage and less copulation success). Grandparental effects of exposure to mint essential oil diminished female grand offspring longevity and improved male copulation behavior parameters such as increased copulation duration and decreased rejection by females. In all, grandparental effects were different in male and female grand offspring based on the essential oil type experienced by F0.

Study of tyramine-binding mechanism and insecticidal activity of oil extracted from Eucalyptus against Sitophilus oryzae.

The rice weevil, Sitophilus oryzae (L.), is a major pest of stored grains throughout the world, which causes quantitative and qualitative losses of food commodities. Eucalyptus essential oils (EOs) possess insecticidal and repellent properties, which make them a potential option for insect control in stored grains with environmentally friendly properties. In the current study, the binding mechanism of tyramine (TA) as a control compound has been investigated by funnel metadynamics (FM) simulation toward the homology model of tyramine1 receptor (TyrR) to explore its binding mode and key residues involved in the binding mechanism. EO compounds have been extracted from the leaf and flower part of Eucalyptus camaldulensis and characterized by GC/MS, and their effectiveness has been evaluated by molecular docking and conventional molecular dynamic (CMD) simulation toward the TyrR model. The FM results suggested that Asp114 followed by Asp80, Asn91, and Asn427 are crucial residues in the binding and the functioning of TA toward TyrR in Sitophilus Oryzae. The GC/MS analysis confirmed a total of 54 and 31 constituents in leaf and flower, respectively, where most of the components (29) are common in both groups. This analysis also revealed the significant concentration of Eucalyptus and α-pinene in leaves and flower EOs. The docking followed by CMD was performed to find the most effective compound in Eucalyptus EOs. In this regard, butanoic acid, 3-methyl-, 3-methyl butyl ester (B12) and 2-Octen-1-ol, 3,7-dimethyl- (B23) from leaf and trans- ß-Ocimene (G04) from flower showed the maximum dock score and binding free energy, making them the leading candidates to replace tyramine in TyrR. The MM-PB/GBSA and MD analysis proved that the B12 structure is the most effective compound in inhibition of TyrR.

Shelf-life extension of pomegranate arils using chitosan nanoparticles loaded with Satureja hortensis essential oil.

BACKGROUND: This study was conducted in two parts to improve the antimicrobial activity and stability of Satureja hortensis essential oil (SEO) and its impacts on the quality of pomegranate arils. In the first part, SEO was encapsulated by an ionic gelation technique into 142.2-267.7 nm chitosan nanoparticles (CSNPs). In the second part of the experiment, the CSNPs and CSNPs-SEO were applied to improve storability of pomegranate arils. Arils were dipped in water (control), CSNPs and CSNPs-SEO for 5 min. After superficial water removal, arils were packed into polystyrene boxes and stored at 5 °C for 18 days. RESULTS: Based on spectrophotometry analysis, the encapsulation efficiency (EE) of SEO-loaded CSNPs (CSNPs-SEO) decreased from 26.57% to 7.41% and their loading capacity (LC) increased from 4.72% to 6.17%, respectively, upon increasing the initial SEO content from 0.125 to 0.5 g g-1 of chitosan. Phytochemicals and water content were maintained, and microbial counts were reduced in the coated arils during storage. Total phenol and antioxidant activity decreased during storage. At the end of storage, the highest total phenol content (2980.0 mg gallic acid equivalents L-1 ) was found in arils treated with CSNPs-SEO, whereas ascorbic acid content was maximal (6.32 mg L-1 ) in arils treated with CSNPs. The encapsulation of savory essential oil in chitosan nanoparticles did not have undesirable effects in pomegranate arils. CONCLUSION: Pre-storage treatment of pomegranate arils with CSNPs-SEO could be considered a beneficial treatment to better maintain the biochemical and sensorial quality during storage. © 2020 Society of Chemical Industry.

Ultrasonic potential in maintaining the quality and reducing the microbial load of minimally processed pomegranate.

Difficulty of Pomegranate fruit peeling and arils separation are the main motivations of progressive ready-to-eat pomegranate fresh arils industry. Also, extracted pomegranate arils are highly perishable due to water loss and microbial contamination expose. The aim of the current work was then to evaluate the effect of ultrasound for 15 and 30 min on maintenance of bioactive compounds and microbial load reduction of pomegranate arils cv. Rabbab. Treated arils were kept at 5 °C and analyzed during 15 days of storage. The most and least weight loss of arils obtained in control and 30 min treated samples, respectively. After 12 days of storage, all samples were decayed except those treated for 15 and 30 min. The ultrasound treatment significantly prevented degradation of anthocyanin and ascorbic acid compounds. Total phenol and antioxidant activity decreased during storage. At the end of storage, the most total phenol content (3898.6 mg GAE L-1) was found in arils treated for 30 min whereas the most anthocyanin (91.93 mg L-1), total antioxidant activity (82.65%), and ascorbic acid (2.53 mg L-1) were found in arils treated for 15 min. Ultrasound treated arils had lower microbial load (total mesophilic bacteria in control and 30-min treated samples) in each stage during storage. At the end of storage, the microbial load in treated and control arils was 0.7 and 0.2 Log CFU g-1, respectively). Overall, ultrasound treatment effectively reduced weight loss and preserved bioactive compounds during storage.

Callosobruchus embryo struggle to guarantee progeny production.

We conducted a series of experiments to test insect embryo capability to survive and increase reproductive investment during early development after short exposure to essential oils. We used Callosobruchus maculatus as a model insect and eucalyptus leaf and flower essential oils. Both essential oils exhibited toxicity against C. maculatus embryos and adults. However, flower essential oil was more toxic. A fetus exposed to essential oils tried to make the best of a bad situation and compensate essential oils harmful effects in the later life stages. Insect progeny production guarantee resulted in a trade-off between reproduction and female longevity. The insect also could alter fitness and reproductive behavior including, mating latency reduction, copulation duration increase, and copulation success rate raise in adulthood. Flower essential oil-exposed embryos were more successful in increasing copulation duration, and leaf essential oil-exposed embryos achieved more copulation success and less mating latency. These consequences persisted until F1 generation that was not directly exposed to essential oil. However, the F2 generation could concur with the harmful effects of essential oils. C. maculatus embryo might use epigenetic mechanisms to guarantee progeny production. Reproductive behavior changes and the trade-off can be evolutionary mechanisms to save species from possible extinction in deleterious situations.

MOLECULAR IDENTIFICATION OF CYSTEINE AND TRYPSIN PROTEASE, EFFECT OF DIFFERENT HOSTS ON PROTEASE EXPRESSION, AND RNAI MEDIATED SILENCING OF CYSTEINE PROTEASE GENE IN THE SUNN PEST.

Sunn pest, Eurygaster integriceps, is a serious pest of cereals in the wide area of the globe from Near and Middle East to East and South Europe and North Africa. This study described for the first time, identification of E. integriceps trypsin serine protease and cathepsin-L cysteine, transcripts involved in digestion, which might serve as targets for pest control management. A total of 478 and 500 base pair long putative trypsin and cysteine gene sequences were characterized and named Tryp and Cys, respectively. In addition, the tissue-specific relative gene expression levels of these genes as well as gluten hydrolase (Gl) were determined under different host kernels feeding conditions. Result showed that mRNA expression of Cys, Tryp, and Gl was significantly affected after feeding on various host plant species. Transcript levels of these genes were most abundant in the wheat-fed E. integriceps larvae compared to other hosts. The Cys transcript was detected exclusively in the gut, whereas the Gl and Tryp transcripts were detectable in both salivary glands and gut. Also possibility of Sunn pest gene silencing was studied by topical application of cysteine double-stranded RNA (dsRNA). The results indicated that topically applied dsRNA on fifth nymphal stage can penetrate the cuticle of the insect and induce RNA interference. The Cys gene mRNA transcript in the gut was reduced to 83.8% 2 days posttreatment. Also, it was found that dsRNA of Cys gene affected fifth nymphal stage development suggesting the involvement of this protease in the insect growth, development, and molting.

Role of dyslipidemia in preeclamptic overweight pregnant women.

BACKGROUND: Obesity is an independent risk factor of preeclampsia with unknown mechanism and hyperlipidemia might be a probable case of it. OBJECTIVE: The objective of this study was to determine the role of hyper-triglyceridemi in association with high prepregnancy body mass index and the risk of preeclampsia. MATERIALS AND METHODS: The authors conducted this case-control study of 42 preeclamptic and 41 normotensive overweight pregnant women. The two groups were comparable with respect to age, gestational age, and body mass index. Blood samples were collected at the time of diagnosis of preeclampsia, after 14 hour fasting to determine plasma lipid concentrations. Enzymatic photometric tests were used to determine lipid profile. Data was analyzed with independent "t-test", Chi-square and one-way ANOVA and post HOC Tukey HSD test. The statistical significance was set at 0.05 levels. RESULTS: In the subjects with preeclampsia, serum triglyceride and total cholesterol levels were significantly increased and plasma HDL-cholesterol concentrations were decreased compared with the controls, (p<0.05), but plasma LDL cholesterol levels didn't differ between the two groups. Women who developed severe preeclampsia had higher concentrations of TG and cholesterol and lower levels of HDL compared to noromotensive group. Mean TG: 375.16 vs. 202.85, p<0.001, Mean cholesterol: 245.64 vs. 214.32, p=0.04, Mean HDL: 40.80 vs. 48.95, p=0.03). CONCLUSION: We noted that dyslipidemia, particularly hypertriglyceridemia was highly correlated with prepregnancy high BMI in preeclamptic women. These findings continue to support a role for dyslipidemia in BMI related preeclampsia.