Applying of SP, DC-Resistivity, DC-TDIP and TDEM soundings in high saline coastal aquifer.

Four surface geophysical techniques were utilized to study the geological and hydrogeological settings of highly saline a coastal aquifer system to the north-east of River Nile Delta, Egypt. These techniques include SP, DC-Resistivity, TDIP and TDEM methods. The first target was to determine the geological stratification as a differentiation among clay, clayey, sand and sandy layers of high saline water. These techniques reflect that there is a complicated lateral and vertical difference in sediments along study area. The surface layers with depth down to ~120 m have low to medium content of clay that change with depth. Then, the second target was the differentiation laterally and vertically for salinity with depth down to ~250m as an interesting hydrogeological setting. These techniques reported that the sediments consist of thin and thick clay and silts, clayey sand, and sandy clay strata. Investigation depth was up to ~210m due to high salinity and clay content effect. At shallow depths, soil texture (down to ~100m and sometimes down to ~160m) consists of clay and silt with sand intercalation. The TEM data indicate a zone of less saline water and low clay content starting from ~40 to ~100m. There may be an evidence for a significant high to medium clay content after these depths down to ~250m. All four methods were calibrated with each other. Accordingly, good matching between the inversion model of TEMSs and composite logs of new drilled well was found, especially in lithological layers identifications. Also, this calibration confirmed that the area was complicated regarding the geological and hydrogeological conditions and the TDIP and TEM are the best methods in studying the environmental, geological and hydrogeological settings as primary important engineering implications for studying coastal highly saline aquifers.

Specifying of aquifer characteristics using forward and inverse modeling of DC-resistivity and TEM methods.

Exploration and determination of the hydro-geo-electrical characteristics of an aquifer can be done by applying the forward (initial multi-layers), and the inverse (final layered) models for interpreting the DC-resistivity (VES) and TEM data. So, 22 VES using Schlumberger configuration (AB/2 = 500-700m) and 12 TEMS using in-loop configuration (square, ℓ = 200m) were carried out at the West El-Minia selected area for studying the Oligocene Clastic and Carbonate aquifers. VESs were interpreted for studying the shallow resistive and conductive layers, as well as faults delineation. TEMs were interpreted for shallow and deep conductive layers discrimination. The VES and TEM inverse models were examined with the drilling data and construed the subsurface into four units; dry Oligocene Clastics (173-467 Ω.m), dry limestone (273-374 Ω.m), saturated Oligocene Clastics (Oligocene aquifer) (2-107 Ω.m), then saturated fractured Eocene limestone to shaly limestone (5-188 Ω.m). Groundwater depths (62-131m) and thicknesses variation were estimated, as well as the faults location. Two hydro-geo-electrical sections were built for simulating the resistivity values and their connotations, and managing in choosing the promised locations for drilling wells. The ramp-off time effect was studied and found that ∼50-∼100m shallow high resistive thickness didn't defined from the TEM data in which the max penetration depth was 672m. The available well logging data were analysed to reveal the pure saturated zones, the volume of shale of 0%-100% and the porosity values of ∼9%-∼35% in the Oligocene aquifer and of ∼4%-∼15.5% in the carbonate aquifer. So, the forward and inverse models application and soundings integration are considered robust tools for estimating and simulating the aquifer characteristics.

Immunohistochemical evaluation of interleukin-23 and cyclooxygenase-2 in the muscles of mice infected with Trichinella spiralis.

The course of Trichinella (T.) spiralis infection includes intestinal and muscle phases. The aims of this work were to evaluate IL-23 and cyclooxygenase-2 (COX-2) by immunohistochemistry in the muscles of T. spiralis infected mice in a time-course study and to correlate their level with the serum levels of IL-23, IFN-γ, IL-4 and IL-10 cytokines. The mice were divided into an un-infected control group (UC) (10 mice) and 5 infected mouse groups (each 10 mice/group. Each mouse was infected with 200 T. spiralis larvae) and sacrificed on days 7, 14, 21, 28 and 35 post-infection (dpi). IL-23 showed weak expression (+1) on the 21st dpi, then it became moderately expressed (+2) on the 28th dpi and on day 35 pi, the immunoreactivity was strong (+3). COX-2 expressed weakly on 14 dpi, while the other mouse groups (21, 28 and 35) showed strong (+3) expression. IL-23 serum concentrations increased gradually in a significant pattern, in comparison to that of UC mice, from the 21st dpi to the end of the experiment. IFN-γ increased gradually and was significantly higher than those of UC mice from the 7th dpi, reached its maximum level on the 21st dpi, after which it decreased non-significantly. IL-4 up-regulated significantly in all infected groups in comparison to UC mice achieving its highest level on the 21st dpi and decreased after that. IL-10 increased significantly on the 7th dpi, but dropped at the 14th dpi, then reached its peak on the 21st dpi, and decreased again on the 28th and 35th dpi. In conclusion, T. spiralis infection caused increased expression of IL-23 and COX-2 in the muscle of infected mice, the effect being strongest on the 35th day. Also, the infection induced a mixed Th1/Th2 profile with a predominance of Th2 at the early muscle phase, after which the immune repose became mainly Th2.

Immunohistochemical evaluation of interleukin-23 and cyclooxygenase-2 in the muscles of mice infected with Trichinella spiralis

@#The course of Trichinella (T.) spiralis infection includes intestinal and muscle phases. The aims of this work were to evaluate IL-23 and cyclooxygenase-2 (COX-2) by immunohistochemistry in the muscles of T. spiralis infected mice in a time-course study and to correlate their level with the serum levels of IL-23, IFN-γ, IL-4 and IL-10 cytokines. The mice were divided into an un-infected control group (UC) (10 mice) and 5 infected mouse groups (each 10 mice/group. Each mouse was infected with 200 T. spiralis larvae) and sacrificed on days 7, 14, 21, 28 and 35 post-infection (dpi). IL-23 showed weak expression (+1) on the 21st dpi, then it became moderately expressed (+2) on the 28th dpi and on day 35 pi, the immunoreactivity was strong (+3). COX-2 expressed weakly on 14 dpi, while the other mouse groups (21, 28 and 35) showed strong (+3) expression. IL-23 serum concentrations increased gradually in a significant pattern, in comparison to that of UC mice, from the 21st dpi to the end of the experiment. IFN-γ increased gradually and was significantly higher than those of UC mice from the 7th dpi, reached its maximum level on the 21st dpi, after which it decreased non-significantly. IL-4 up-regulated significantly in all infected groups in comparison to UC mice achieving its highest level on the 21st dpi and decreased after that. IL-10 increased significantly on the 7th dpi, but dropped at the 14th dpi, then reached its peak on the 21st dpi, and decreased again on the 28th and 35th dpi. In conclusion, T. spiralis infection caused increased expression of IL-23 and COX-2 in the muscle of infected mice, the effect being strongest on the 35th day. Also, the infection induced a mixed Th1/Th2 profile with a predominance of Th2 at the early muscle phase, after which the immune repose became mainly Th2.