Compartmentalization proteomics revealed endolysosomal protein network changes in a goat model of atrial fibrillation.

Endolysosomes (EL) are known for their role in regulating both intracellular trafficking and proteostasis. EL facilitate the elimination of damaged membranes, protein aggregates, membranous organelles and play an important role in calcium signaling. The specific role of EL in cardiac atrial fibrillation (AF) is not well understood. We isolated atrial EL organelles from AF goat biopsies and conducted a comprehensive integrated omics analysis to study the EL-specific proteins and pathways. We also performed electron tomography, protein and enzyme assays on these biopsies. Our results revealed the upregulation of the AMPK pathway and the expression of EL-specific proteins that were not found in whole tissue lysates, including GAA, DYNLRB1, CLTB, SIRT3, CCT2, and muscle-specific HSPB2. We also observed structural anomalies, such as autophagic-vacuole formation, irregularly shaped mitochondria, and glycogen deposition. Our results provide molecular information suggesting EL play a role in AF disease process over extended time frames.

Perceived antimicrobial dispensing practices in medicine outlets in Ghana: A maximum difference experiment design.

INTRODUCTION: Antimicrobials are consumed among patients globally, but in developing and middle-income countries, these drugs can be obtained without a prescription from pharmacies and licensed drug stores due to inadequate regulation in the pharmaceutical sector. This study aimed to assess antimicrobial dispensing practices in medicine sales outlets (i.e., pharmacies and licensed drug stores) to provide quantitative evidence for policy discussions to enhance patient safety and care quality in Ghana's pharmaceutical industry. METHOD: The data for this study were obtained from a cross-sectional survey conducted in the Greater Accra region between July and August 2022. The survey was conducted through interviewer-administered questionnaires, and 200 staff members from medicine sales outlets were randomly selected using a two-stage cluster and random sampling technique. The maximum difference experiment model, rooted in random utility theory, was used to analyze their antimicrobial dispensing practices. RESULT: We found that medicine sales outlet staffs were highly concerned about following the drug act and not dispensing antimicrobials without a prescription, and usually refer a patient to get a prescription from a doctor if the patient has complications (like high fever, generalized malaise, fatigue as symptoms, sinusitis). Stronger concerns were also observed for medicine outlet staff not dispensing antimicrobials without a prescription if the patient is pediatric or geriatric with a severe infection. They also evaluated patients and dispensed antimicrobials based on symptoms, not their age or gender. However, they tended not to dispense antibiotics if the patient had a mild fever and requested it without a prescription. CONCLUSION: Our results provide insight into the need for a national surveillance system for monitoring antimicrobial prescribing and dispensing practices at medicine sales outlets. Therefore, the selection of antimicrobials for treating infectious diseases may be informed by evidence-based antimicrobial prescribing and dispensing surveillance data and will help policymakers to know the pattern of commonly consumed antimicrobials in the medicine sales outlets.

The use of statistical methods to assess groundwater contamination in the Lower Tano river basin, Ghana, West Africa.

In this study, descriptive statistics, correlation matrix, multiple regression model, and geostatistical models were used to assess the contamination of groundwater with respect to trace elements in the Lower Tano river basin, Ghana, West Africa. A total number of 48 boreholes drilled across the basin with depths ranging from 18 to 60 m were used as data sources in this study. The results of the descriptive statistics showed that the average lead, iron, and aluminium concentrations exceeded the WHO permissible limits of 0.3 mg/L, 0.01 mg/L, and 0.2 mg/L respectively. Furthermore, copper, chromium, aluminium, zinc, manganese, nickel, iron, arsenic, electrical conductivity, and total dissolved solids were found to be extreme and highly positively skewed. Even though significant correlations exist among some variables, the statistical results showed that the quality of the boreholes drilled across the basin was mainly originating from geogenic and anthropogenic sources. In addition, each pair of correlated physical parameters and trace elements in the drilled boreholes were predicted using multiple regression models. Likewise, geostatistical modelling was used to assess the spatial analysis of each pair of correlated physical parameters and trace elements in the drilled boreholes. The cross-validation results revealed kriging model, as the most precise model for the spatial distribution maps for the correlated physical parameters, and correlated trace elements concentration in the boreholes drilled across the study region. The semivariogram models showed that most of the correlated physical parameters and correlated trace elements were weak moderately and strongly spatially dependent, suggesting fewer agronomic influences. The results of the spatial analysis were consistent with the multiple regression model and the Pearson correlation matrix.

Modeling the Amount of Waste Generated by Households in the Greater Accra Region Using Artificial Neural Networks.

Waste can be defined as solids or liquids unwanted by members of the society and meant to be disposed. In developing countries such as Ghana, the management of waste is the responsibility of the metropolitan authorities. These authorities do not seem to have effective management of the waste situation, and therefore, it is not unusual to see waste clog the drains and litter the streets of the capital city, Accra. The impact of waste on the environment, along with its associated health-related problems, cannot be overemphasized. The Joint Monitoring Programme report in 2015 ranked Ghana as the seventh dirtiest country in the world. The lack of effective waste management planning is evident in the large amount of waste dumped in open areas and gutters that remains uncollected. In planning for solid waste management, reliable data concerning waste generation, influencing factors on waste generation, and a reliable forecast of waste quantities are required. This study used two algorithms, namely, Levenberg-Marquardt and the Bayesian regularization, to estimate the parameters of an artificial neural network model fitted to predict the average monthly waste generated and critically assess the factors that influence solid waste generation in some selected districts of the Greater Accra region. The study found Bayesian regularization algorithm to be suitable with the minimum mean square error of 104.78559 on training data and 217.12465 on test data and higher correlation coefficients (0.99801 on training data, 0.99570 on test data, and 0.99767 on the overall data) between the target variables (average monthly waste generated) and the predicted outputs. House size, districts, employment category, dominant religion, and house type with respective importance of 0.56, 0.172, 0.061, 0.027, and 0.026 were found to be the top five important input variables required for forecasting household waste. It is recommended that efforts of the government and its stakeholders to reduce the amount of waste generated by households be directed at providing bins, increasing the frequency of waste collection (especially in highly populated areas), and managing the economic activities in the top five selected districts (Ledzekuku Krowor, Tema West, Asheidu Keteke, Ashaiman, and Ayawaso West), amongst others.

Joint large deviation result for empirical measures of the coloured random geometric graphs.

We prove joint large deviation principle for the empirical pair measure and empirical locality measure of the near intermediate coloured random geometric graph models on n points picked uniformly in a d-dimensional torus of a unit circumference. From this result we obtain large deviation principles for the number of edges per vertex, the degree distribution and the proportion of isolated vertices for the near intermediate random geometric graph models.

Modeling inflation rates and exchange rates in Ghana: application of multivariate GARCH models.

This paper was aimed at investigating the volatility and conditional relationship among inflation rates, exchange rates and interest rates as well as to construct a model using multivariate GARCH DCC and BEKK models using Ghana data from January 1990 to December 2013. The study revealed that the cumulative depreciation of the cedi to the US dollar from 1990 to 2013 is 7,010.2% and the yearly weighted depreciation of the cedi to the US dollar for the period is 20.4%. There was evidence that, the fact that inflation rate was stable, does not mean that exchange rates and interest rates are expected to be stable. Rather, when the cedi performs well on the forex, inflation rates and interest rates react positively and become stable in the long run. The BEKK model is robust to modelling and forecasting volatility of inflation rates, exchange rates and interest rates. The DCC model is robust to model the conditional and unconditional correlation among inflation rates, exchange rates and interest rates. The BEKK model, which forecasted high exchange rate volatility for the year 2014, is very robust for modelling the exchange rates in Ghana. The mean equation of the DCC model is also robust to forecast inflation rates in Ghana.