Sacha inchi albumin delays skin-aging by alleviating inflammation, oxidative stress and regulating gut microbiota in d-galactose induced-aging mice.

BACKGROUND: Sacha inchi albumin exhibits considerable functional activity with notable anti-inflammatory and antioxidation properties, which could delay skin aging. However, its underlying mechanisms for delaying skin aging have not been elucidated. The aim of the present study was to investigate the anti-skin-aging effect of sacha inchi albumin (SIA) in d-galactose induced-aging mice. RESULTS: Sacha inchi albumin improved moisture content, collagen level, and the state of aged skin in rats. Sacha inchi albumin intervention markedly increased the skin antioxidant enzymatic activities including those of glutathione peroxidase, and catalase, but decreased the malondialdehyde content. It also regulated inflammation by reducing the level of tumor necrosis factor-α (TNF-α) and increasing the level of interleukin-6 (IL-6). Administration of SIA also increased the expression level of collagen I and III, increased the expression of tissue inhibitor of metalloprotease-1, and decreased the expression of metalloproteinases. Sacha inchi albumin can also activate the transforming growth factor-ß (TGF-ß)/Smad pathway. Meanwhile, 16S rRNA sequencing analysis revealed that SIA treatment altered the composition of microbiota, and increased the relative abundance of Lactobacillus, but decreased the relative abundance of Alloprevotella and Helicobacter, etc. Helicobacter was positively associated with malondialdehyde (MDA) content and was negatively related to IL-6. CONCLUSION: Sacha inchi albumin exhibits excellent anti-skin-aging effect, which provide a new insight for the development of functional sacha inchi albumin. © 2023 Society of Chemical Industry.

Characterization of antidiabetic effects of Dendrobium officinale derivatives in a mouse model of type 2 diabetes mellitus.

In this research, two sequential Dendrobium officinale water extracts (WDOE and WDOP1) were shown to significantly ameliorate type 2 diabetic mellitus (T2DM) in a mouse model. WDOP1 was identified as a glucomannan with a backbone of 1,4-linked Manp and 1,4-linked Glcp and an average molecular weight of 731 kDa. We also found that both WDOE and WDOP1 could significantly alleviate glucose intolerance, insulin resistance, oxidative stress injury, serum lipid metabolism disturbances, and histopathological damage in T2DM mice. In addition, we demonstrated that WDOE and WDOP1 reversed gut dysbiosis by reshaping the microbiota spectrum in T2DM mice. It should be emphasized that both Dendrobium officinale extracts afforded beneficial effects in T2DM mice comparable to metformin, despite differences in examined dosages. In conclusion, we demonstrated that Dendrobium officinale derivatives have potential as T2DM management nutraceuticals.

Effect of steam explosion pretreatment on polysaccharide isolated from Poria cocos: Structure and immunostimulatory activity.

This study aimed to examine the effects of steam explosion (SE) pretreatment on the structural characteristics and immunostimulatory activity of polysaccharide from Poria cocos. Results showed that the average molecular weights of native polysaccharide (PCP) and SE-pretreated polysaccharide (SEPCP) were 18.67 and 6.52 kDa, respectively. PCP and SEPCP shared the same profiles of monosaccharides (mannose, glucose, galactose, and fucose) in different composition ratios, that is, PCP in a molar percentage of 13.5:33:40.3:13.2 and SEPCP in a molar percentage of 2.1:90.3:5.8:1.8. The surface structure of PCP showed smooth and densely spherical particles, whereas SEPCP had a rough surface and porous honeycomb structure. The main linkage types of PCP comprised 1,6-α-d-Galp, 1,2,6-α-d-Glcp, and T-α-d-Manp, whereas SEPCP primarily contained 1,3-ß-d-Glcp backbone and T-ß-d-Glcp branches. Compared with PCP, we further revealed that SEPCP had a better immune enhancement on the phagocytic ability, NO production, and the secretion levels of TNF-α and IL-6 in RAW 264.7 cells. Collectively, our observations supported that SE pretreatment could help to change the structure and improve the immunostimulatory activity of polysaccharide from P. cocos. PRACTICAL APPLICATIONS: SE technology is extensively used to extract bioactive components with improved yields owing to this technology's benefits of low energy consumption and high efficiency. SE pretreatment was found to contribute to the destruction of cell-wall structure, which could help to enhance the extraction yields of P. cocos polysaccharide (PCP). Meanwhile, SE pretreatment also could change the structural features and improve the immunostimulatory activity of PCP. This study revealed that more bioactive PCP with strengthened immunoregulatory effect was obtained pretreated by SE. This study was able to provide the effective information on the application of steam explosion technology to promote the further development and utilization of PCP in the pharmaceutical and functional food fields.