1.
Sheng Wu Gong Cheng Xue Bao
; 16(1): 65-8, 2000 Jan.
Artigo
em Chinês
| MEDLINE
| ID: mdl-10883279
RESUMO
The biological and immunological characteristics of HIV-1 core protein p17-24 expressed by recombinant vaccinia viruses were studied. The results of indirect immunofluorescence assay (I-IFA), Western blot and dot ELISA showed that the two recombinants could express the p24 gag and p17-24 gag fusion proteins in infected cell lines respectively. The electromicroscope observation revealed that the expressed proteins could also assemble into virus-like particles. The recombinant vaccinia viruses can also stimulate mice for the formation of anti HIV-1 Gag p24 antibody. When infected with the recombinant viruses, the chromosome DNA ladder caused by the apoptosis of the BHK cell was observed.