RESUMO
Marine seaweeds contain a valuable source of functional bioactive polysaccharide and it plays main role for effective anticancer activity. The structural feature of SPs was studied through FT-IR and 1H NMR spectra analysis. The isolated SPs from A. spicifera contain 63.3% of total sugar, 21.9% of total sulfate and 12.6% of total uranic acid was found. The active F2 fraction molecular weight of SP was found to be 420â¯kDa. The sugar was composed of galactose (73.5%), xylose (9.2%), mannose (1.9%) and arabinose (10.9%). Further the SP showed DPPH free radical scavenging activity of 55.55% at 150⯵g/mL and reducing power activity of 91.3% at 125⯵g/mL. In the present study, the purified sulfated polysaccharide (fraction F2) were extracted, purified and characterized for red seaweed and evaluated for their potential anticancer activity of in A549 cell lines under in vitro condition. These polysaccharide fractions exhibited potential apoptotic effects on A549 cell lines.
Assuntos
Antioxidantes/química , Sequestradores de Radicais Livres/química , Polissacarídeos/química , Rodófitas/química , Antioxidantes/isolamento & purificação , Compostos de Bifenilo/química , Compostos de Bifenilo/isolamento & purificação , Carboidratos/química , Carboidratos/isolamento & purificação , Sequestradores de Radicais Livres/isolamento & purificação , Galactose/química , Galactose/isolamento & purificação , Peso Molecular , Polissacarídeos/isolamento & purificação , Espectroscopia de Infravermelho com Transformada de Fourier , Sulfatos/química , Xilose/químicaRESUMO
The experimental results revealed that the 2.27-fold lipid yield was enhanced in nitrogen-depleted condition (226 mg/L) when compared to nitrogen rich condition (99.33 mg/L). Since specific growth rate (SGR) is the single most criteria to decide the biomass yield of microalgae, SGR was analyzed. SGR of 0.33 day(-1) was observed with 0.47 doubling per day under nitrogen rich condition. However, low growth rate of 0.14 day(-1) with a doubling of 0.20 per day was recorded under nitrogen depleted condition. In accordance with SGR, the dry biomass yield was ranged from 0.055 ± 0.005 to 1.394 ± 0.010 g/L in the presence of nitrogen, indicating the essentiality of major nutrient in the growth medium. The enhanced lipid accumulation under nitrogen starved condition by Scenedesmus quadricauda was perhaps achieved by adopting adverse environmental condition and possible increment in cell size in terms of length/breadth ratio (1.11).