Using biofilm as a novel approach to assess stormwater treatment efficacy.

Contaminants associated with stormwater are among the leading causes of water quality impairment in urban streams. Multiple device treatment systems are commonly installed with the aim of reducing contaminant loads within stormwater discharge. However, the in situ performance of such systems remains poorly understood. We investigated the efficacy of an advanced stormwater treatment system by monitoring biofilm associated metals and biofilm bacterial community composition at multiple locations through the treatment system (which included rain gardens, grassy swales, a stormwater filter and a wetland) and in the receiving stream above and below the stormwater discharge. Changes in bacterial community composition were assessed by Automated Ribosomal Intergenic Spacer Analysis (ARISA) and concentrations of biofilm associated metals monitored by Inductively Coupled Plasma-Mass Spectrometry (ICP-MS). Significant differences in bacterial community composition were detected throughout the stormwater network. Bacterial communities gradually changed towards a community more similar to that within the receiving stream and the discharge of treated stormwater had little effect on the composition of bacterial communities in the receiving stream, suggesting the effective conditioning of water quality by the treatment system. Concentrations of some biofilm-associated metals declined following sequential treatment, for example copper (73% reduction), zinc (48% reduction) and lead (46% reduction). In contrast, levels of arsenic, cadmium, chromium and nickel were not reduced by the treatment system. We demonstrate that biofilm bacterial community composition is a sensitive indicator of environmental changes within freshwater ecosystems and an efficient indicator to monitor water quality in enclosed stormwater networks where traditional biological indicators are not available.

Metal concentrations in stream biofilm and sediments and their potential to explain biofilm microbial community structure.

Concentrations of metals associated with sediments have traditionally been analysed to assess the extent of heavy metal contamination in freshwater environments. Stream biofilms present an alternative medium for this assessment which may be more relevant to the risk incurred by stream ecosystems as they are intensively grazed by aquatic organisms at a higher trophic level. Therefore, we investigated zinc, copper and lead concentrations in biofilms and sediments of 23 stream sites variously impacted by urbanisation. Simultaneously, biofilm bacterial and ciliate protozoan community structure was analysed by Automated Ribosomal Intergenic Spacer Analysis and Terminal Restriction Fragment Length Polymorphism, respectively. Statistical analysis revealed that biofilm associated metals explained a greater proportion of the variations observed in bacterial and ciliate communities than did sediment associated-metals. This study suggests that the analysis of metal concentrations in biofilms provide a good assessment of detrimental effects of metal contaminants on aquatic biota.

Three common metal contaminants of urban runoff (Zn, Cu & Pb) accumulate in freshwater biofilm and modify embedded bacterial communities.

We investigated the absorption rates of zinc, copper and lead in freshwater biofilm and assessed whether biofilm bacterial populations are affected by exposure to environmentally relevant concentrations of these metals in flow chamber microcosms. Metals were rapidly accumulated by the biofilm and then retained for at least 14 days after transfer to uncontaminated water. Changes in bacterial populations were assessed by Automated Ribosomal Intergenic Spacer Analysis (ARISA) and 16S rRNA gene clone libraries. Significant differences in bacterial community structure occurred within only three days of exposure to metals and remained detectable at least 14 days after transfer to uncontaminated water. The rapid uptake of stormwater-associated metals and their retention in the biofilm highlight the potential role of biofilms in the transfer of metals to organisms at higher trophic levels. The sensitivity of stream biofilm bacterial populations to metal exposure supports their use as an indicator of stream ecological health.